公开(公告)号：WO2005050209A1

公开(公告)日：2005-06-02

申请号：PCT/SE2004/001697

申请日：2004-11-19

Applicant: BIOSENSOR APPLICATIONS SWEDEN (PUBL) ,  MÅNSSON, Per ,  BERGGREN, Daniel ,  LUNDBERG, Carl ,  HELLGREN, Ann-Charlotte ,  HÖJVALL, Lena

Inventor： MÅNSSON, Per ,  BERGGREN, Daniel ,  LUNDBERG, Carl ,  HELLGREN, Ann-Charlotte ,  HÖJVALL, Lena

IPC: G01N33/543

CPC classification number: G01N29/022 ,  G01N29/222 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0426

Abstract: A mixture of isolated or synthetic affinity molecules in a liquid carrier is disclosed. The mixture comprises at least two different affinity molecules, each with affinity for a predetermined analyte, for use in a single or multi flow cell piezoelectric crystal micro balance apparatus. Each isolated or synthetic affinity molecule forms molecule forms together with the predetermined analyte an interaction pair selected from the group consisting of anion-cation, antibody-antigen, receptor-ligand, enzyme-substrate, oligonucleotide-oligonucleotide with complementary sequence, oligonucleotide-protein, oligonucleotide-cell, and peptide nucleic acid (PNA) oligomer - polynucleotide, wherein the polynucleotide may be selected from the group consisting of RNA, DNA and PNA polymers complementary to the PNA oligomer. Use of the mixture for introduction into the liquid flow of a single or multi flow cell piezoelectric crystal micro balance apparatus is also described, as well as a kit containing the mixture.

Abstract translation: 公开了液体载体中分离的或合成的亲和分子的混合物。 该混合物包含至少两种不同的亲和力分子，各自对于预定的分析物具有亲和力，用于单流或多流量单元压电晶体微量平衡装置。 每个分离或合成的亲和分子与预定分析物一起形成分子形式，所述分子形式选自阴离子阳离子，抗体 - 抗原，受体 - 配体，酶 - 底物，具有互补序列的寡核苷酸 - 寡核苷酸，寡核苷酸 - 蛋白质， 寡核苷酸细胞和肽核酸（PNA）寡聚体 - 多核苷酸，其中多核苷酸可以选自与PNA低聚物互补的RNA，DNA和PNA聚合物。 还描述了将混合物引入单流动池或多流通池压电晶体微量平衡装置的液体流中的用途，以及含有该混合物的试剂盒。