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公开(公告)号：WO2013007574A1
公开(公告)日：2013-01-17
申请号：PCT/EP2012/063034
申请日：2012-07-04
Applicant: LONZA COLOGNE GMBH , SCHENK, Judith , VAN DEN BOS, Christian , ROSENBAUM, Claudia , BRILL, Silke , SAFTA, Jade
Inventor： SCHENK, Judith , VAN DEN BOS, Christian , ROSENBAUM, Claudia , BRILL, Silke , SAFTA, Jade
IPC: C12M3/02
Abstract: The invention relates to a method and device for cultivating attachment-dependent cells in the presence of microcarriers in a flexible cultivation container. In particular, the invention concerns a cultivation method using a flexible, scalable, disposable, substantially gas-permeable and non-liquid-permeable container for expansion of adherent cells on microcarriers. According to the invention, cultivation of the cells is accomplished under orbital shaking at 8 rpm or more than 8 rpm.
Abstract translation: 本发明涉及一种用于在柔性培养容器中的微载体存在下培养附着依赖细胞的方法和装置。 特别地，本发明涉及一种使用柔性的，可缩放的，一次性的，基本上透气的和非液体可渗透的容器用于在微载体上扩张贴壁细胞的培养方法。 根据本发明，在8rpm或大于8rpm的轨道振荡下实现细胞的培养。
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公开(公告)号：WO2013007574A9
公开(公告)日：2013-03-07
申请号：PCT/EP2012063034
申请日：2012-07-04
Applicant: LONZA COLOGNE GMBH , SCHENK JUDITH , VAN DEN BOS CHRISTIAN , ROSENBAUM CLAUDIA , BRILL SILKE , SAFTA JADE
Inventor： SCHENK JUDITH , VAN DEN BOS CHRISTIAN , ROSENBAUM CLAUDIA , BRILL SILKE , SAFTA JADE
IPC: C12M3/02
Abstract: The invention relates to a method and device for cultivating attachment-dependent cells in the presence of microcarriers in a flexible cultivation container. In particular, the invention concerns a cultivation method using a flexible, scalable, disposable, substantially gas-permeable and non-liquid-permeable container for expansion of adherent cells on microcarriers. According to the invention, cultivation of the cells is accomplished under orbital shaking at 8 rpm or more than 8 rpm.
Abstract translation: 本发明涉及在柔性栽培容器中在微载体存在下培养附着依赖细胞的方法和装置。 具体而言，本发明涉及一种使用柔性的，可伸缩的，一次性的，基本上透气且不可渗透液体的容器用于在微载体上膨胀贴壁细胞的培养方法。 根据本发明，细胞的培养在8rpm或8rpm以上的轨道振动下完成。
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