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    METHOD FOR CONTROLLING BINDING OF CELLS TO A SUBSTRATE
    1.
    发明申请
    METHOD FOR CONTROLLING BINDING OF CELLS TO A SUBSTRATE 审中-公开
    用于控制细胞与基底结合的方法

    公开(公告)号：WO2012062819A3

    公开(公告)日：2012-07-12

    申请号：PCT/EP2011069773

    申请日：2011-11-09

    Applicant: LONZA COLOGNE GMBH ,  SCHENK JUDITH ,  VAN DEN BOS CHRISTIAN ,  ROSENBAUM CLAUDIA ,  NIE YING

    Inventor： SCHENK JUDITH ,  VAN DEN BOS CHRISTIAN ,  ROSENBAUM CLAUDIA ,  NIE YING

    IPC: C12N5/0775 ,  C12N5/0735

    CPC classification number: C12N5/0675 ,  C12N5/0606 ,  C12N5/0663 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2531/00 ,  C12N2533/30 ,  C12N2533/90

    Abstract: The invention relates to a method for promoting the adhesion of cells to a substrate to which these cells usually have no or only low affinity, wherein the adhesion of the cells to the substrate is promoted by supplying the cells with the non-muscle myosin II inhibitor Blebbistatin so as to enable the cells to attach to surfaces to which they otherwise would not have sufficient affinity. Surprisingly, supplying the cells with the inhibitor enhances the capability of these cells to attach to surfaces to which they usually have no or only low affinity, for example, PTFE (Teflon®). The invention further concerns uses of the non- muscle myosin II inhibitor Blebbistatin and devices having at least one surface which is coated with cells that have no or only low affinity to said surface.

    Abstract translation: 本发明涉及促进细胞对这些细胞通常没有或仅具有低亲和力的底物的粘附的方法，其中通过向非细胞肌球蛋白II抑制剂供体细胞来促进细胞对底物的粘附 Blebbistatin，以便使细胞附着于否则不具有足够亲和力的表面。 令人惊奇的是，向细胞提供抑制剂增强了这些细胞附着于通常不具有或仅具有低亲和力的表面（例如PTFE）的能力。 本发明还涉及非肌肉肌球蛋白II抑制剂Blebbistatin和具有至少一个表面的装置的使用，所述表面涂覆有对所述表面没有或仅具有低亲和力的细胞。

    SCALE UP OF CELL CULTURES
    5.
    发明申请
    SCALE UP OF CELL CULTURES 审中-公开
    细胞培养的规模

    公开(公告)号：WO2013007574A9

    公开(公告)日：2013-03-07

    申请号：PCT/EP2012063034

    申请日：2012-07-04

    Applicant: LONZA COLOGNE GMBH ,  SCHENK JUDITH ,  VAN DEN BOS CHRISTIAN ,  ROSENBAUM CLAUDIA ,  BRILL SILKE ,  SAFTA JADE

    Inventor： SCHENK JUDITH ,  VAN DEN BOS CHRISTIAN ,  ROSENBAUM CLAUDIA ,  BRILL SILKE ,  SAFTA JADE

    IPC: C12M3/02

    CPC classification number: C12M25/16 ,  C12M23/26 ,  C12M27/16 ,  C12M41/12 ,  C12M41/34 ,  C12N2531/00

    Abstract: The invention relates to a method and device for cultivating attachment-dependent cells in the presence of microcarriers in a flexible cultivation container. In particular, the invention concerns a cultivation method using a flexible, scalable, disposable, substantially gas-permeable and non-liquid-permeable container for expansion of adherent cells on microcarriers. According to the invention, cultivation of the cells is accomplished under orbital shaking at 8 rpm or more than 8 rpm.

    Abstract translation: 本发明涉及在柔性栽培容器中在微载体存在下培养附着依赖细胞的方法和装置。 具体而言，本发明涉及一种使用柔性的，可伸缩的，一次性的，基本上透气且不可渗透液体的容器用于在微载体上膨胀贴壁细胞的培养方法。 根据本发明，细胞的培养在8rpm或8rpm以上的轨道振动下完成。

    METHOD FOR CONTROLLING BINDING OF CELLS TO A SUBSTRATE
    9.
    发明申请
    METHOD FOR CONTROLLING BINDING OF CELLS TO A SUBSTRATE 审中-公开
    控制细胞与基质结合的方法

    公开(公告)号：WO2012062819A2

    公开(公告)日：2012-05-18

    申请号：PCT/EP2011/069773

    申请日：2011-11-09

    Applicant: LONZA COLOGNE GMBH ,  SCHENK, Judith ,  VAN DEN BOS, Christian ,  ROSENBAUM, Claudia ,  NIE, Ying

    Inventor： SCHENK, Judith ,  VAN DEN BOS, Christian ,  ROSENBAUM, Claudia ,  NIE, Ying

    IPC: C12N5/0775 ,  C12N5/0735

    CPC classification number: C12N5/0675 ,  C12N5/0606 ,  C12N5/0663 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2531/00 ,  C12N2533/30 ,  C12N2533/90

    Abstract: The invention relates to a method for promoting the adhesion of cells to a substrate to which these cells usually have no or only low affinity, wherein the adhesion of the cells to the substrate is promoted by supplying the cells with the non-muscle myosin II inhibitor Blebbistatin so as to enable the cells to attach to surfaces to which they otherwise would not have sufficient affinity. Surprisingly, supplying the cells with the inhibitor enhances the capability of these cells to attach to surfaces to which they usually have no or only low affinity, for example, PTFE (Teflon ® ). The invention further concerns uses of the non- muscle myosin II inhibitor Blebbistatin and devices having at least one surface which is coated with cells that have no or only low affinity to said surface.

    Abstract translation: 本发明涉及一种促进细胞与这些细胞通常不具有或仅具有低亲和力的基质粘附的方法，其中细胞与基质的粘附通过供给细胞 与非肌肉肌球蛋白II抑制剂布雷他汀联合使用，以使细胞能够附着到它们不具有足够亲和力的表面上。 令人惊讶的是，向细胞中加入抑制剂可增强这些细胞附着在通常不具有或仅具有低亲和力的表面上的能力，例如PTFE（Teflon®）。 本发明进一步涉及非肌肉肌球蛋白II抑制剂布雷他汀的用途以及具有至少一个表面的装置，所述表面涂覆有对所述表面没有或仅具有低亲和力的细胞。

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