公开(公告)号：WO2010048398A3

公开(公告)日：2010-06-24

申请号：PCT/US2009061675

申请日：2009-10-22

Applicant: UNIV CORNELL ,  JAHN MARGARET ,  CAVATORTA JASON

Inventor： JAHN MARGARET ,  CAVATORTA JASON

IPC: C07H21/00 ,  C12N15/00

CPC classification number: C07K14/415 ,  C12N15/8283

Abstract: A nucleic acid molecule encoding a mutant form of wild-type translation initiation factor elF4E is disclosed A mutation comprising one or more of I70N, I70E, L82R, DI12N, L48F, S68K, A77D or MI09I in SEQ ID NO 1 imparts virus resistance to potato Nucleic acid constructs, expression vectors, cells, plants, and plant seeds containing the nucleic acid sequence are also disclosed, as are methods of imparting virus resistance to plants

Abstract translation: 公开了编码野生型翻译起始因子elF4E的突变形式的核酸分子，其包含SEQ ID NO 1中的I70N，I70E，L82R，DI12N，L48F，S68K，A77D或MI09I中的一个或多个的突变赋予对马铃薯的病毒抗性 还公开了含有核酸序列的核酸构建体，表达载体，细胞，植物和植物种子，以及赋予植物病毒抗性的方法

公开(公告)号：WO2010048398A2

公开(公告)日：2010-04-29

申请号：PCT/US2009/061675

申请日：2009-10-22

Applicant: CORNELL UNIVERSITY ,  JAHN, Margaret ,  CAVATORTA, Jason

Inventor： JAHN, Margaret ,  CAVATORTA, Jason

IPC: A01H5/00

CPC classification number: C07K14/415 ,  C12N15/8283

Abstract: The present invention relates to a nucleic acid molecule encoding a mutant form of wild-type translation initiation factor eIF4E, which has an amino acid sequence of SEQ ID NO: 1, wherein the mutant form imparts virus resistance to potato and has a mutation with respect to the wild-type amino acid sequence of SEQ ID NO: 1 selected from the group consisting of: (1) any one or more of I70N, I70E, L82R, and Dl 12N and (2) any one or more of L48F, S68K, A77D, and M109I. Nucleic acid constructs, expression vectors, cells, plants, and plant seeds containing the nucleic acid molecule of the present invention, are also disclosed, as are methods of imparting virus resistance to plants.

Abstract translation: 本发明涉及编码具有SEQ ID NO：1的氨基酸序列的野生型翻译起始因子eIF4E的突变形式的核酸分子，其中突变形式赋予对马铃薯的病毒抗性，并且具有相对于 至选自以下的SEQ ID NO：1的野生型氨基酸序列：（1）I70N，I70E，L82R和D112N中的任何一种或多种，​​和（2）L48F，S68K中的任何一种或多种 ，A77D和M109I。 还公开了含有本发明的核酸分子的核酸构建体，表达载体，细胞，植物和植物种子，以及赋予植物病毒抗性的方法。