公开(公告)号：WO2005077077A2

公开(公告)日：2005-08-25

申请号：PCT/US2005004256

申请日：2005-02-09

Applicant: CELLULAR TECHNOLOGY LTD ,  LEHMANN PAUL V

Inventor： LEHMANN PAUL V

IPC: G08B25/01 ,  H04M11/04

CPC classification number: H04M11/04 ,  G08B13/19621 ,  G08B21/0269 ,  G08B21/028 ,  G08B21/0294 ,  G08B25/016

Abstract: Embodiments include systems and methods for summoning help and locating a person, comprising transmitting information over a wireless network in response to a user action; wherein the information includes the geographic location of the user.

Abstract translation: 实施例包括用于召唤人的帮助和定位的系​​统和方法，包括响应于用户动作通过无线网络发送信息; 其中所述信息包括所述用户的地理位置。

公开(公告)号：WO2006014925A3

公开(公告)日：2006-03-30

申请号：PCT/US2005026435

申请日：2005-07-25

Applicant: CELLULAR TECHNOLOGY LTD ,  LEHMANN PAUL V ,  KARULIN ALEXEY Y ,  VELASCO JR VIRGILIO B ,  KLEEN THOMAS OLIVER

Inventor： LEHMANN PAUL V ,  KARULIN ALEXEY Y ,  VELASCO JR VIRGILIO B ,  KLEEN THOMAS OLIVER

IPC: H04N1/04

CPC classification number: H04N1/02815 ,  G01N21/253 ,  G01N21/255 ,  G01N21/6456 ,  H04N1/00795 ,  H04N1/00827 ,  H04N1/02845 ,  H04N1/1013

Abstract: Embodiments of this invention include flatbed scanners that have a surface and have a plurality of light sources. Alternatively, a single light source can be moved in two dimensions relative to an object placed on the surface of the scanner. Other embodiments include a scan head that is moveable in two dimensions relative to the flat bed of the scanner. Further embodiments include one or more polarizing filters to reduce glare and increase the quality of a captured image. Theses scanners can be used to capture images of enzyme linked immunosorbent assay (ELISA) substrates, plates having cultures thereon or other objects having irregular lower surfaces. Additional embodiments include methods for capturing images from ELISA substrates, bacterial culture plates, viral plaque assay plates and the like.

Abstract translation: 本发明的实施例包括具有表面并具有多个光源的平板扫描仪。 或者，单个光源可以相对于放置在扫描仪表面上的物体在二维上移动。 其他实施例包括可相对于扫描仪的平台在二维上移动的扫描头。 进一步的实施例包括一个或多个偏振滤光片以减少眩光并增加捕获图像的质量。 这些扫描仪可用于捕获酶联免疫吸附测定（ELISA）底物，其上具有培养物的平板或其他具有不规则下表面的物体的图像。 另外的实施方案包括用于从ELISA底物，细菌培养板，病毒空斑测定板等捕获图像的方法。

公开(公告)号：WO2005012505A3

公开(公告)日：2005-06-16

申请号：PCT/US2004023582

申请日：2004-07-23

Applicant: CELLULAR TECHNOLOGY LTD ,  LEHMANN PAUL V ,  KARULIN ALEXEY Y ,  KLEEN THOMAS OLIVER

Inventor： LEHMANN PAUL V ,  KARULIN ALEXEY Y ,  KLEEN THOMAS OLIVER

IPC: G01N33/50 ,  G01N33/533 ,  G01N33/58 ,  G01N3/58

CPC classification number: G01N33/505 ,  B82Y15/00 ,  G01N33/587 ,  G01N33/588 ,  G01N2333/5409 ,  G01N2333/57 ,  Y10T436/25375

Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.

Abstract translation: 本发明的实施方式涉及使用具有产品特异性检测试剂并具有特征性光谱特征的检测颗粒来检测细胞产物的装置和方法。 具体而言，提供了用于测量包括细胞因子的分泌细胞产物的装置和方法。 检测底物包括在其上具有产物特异性捕获试剂或包含疏水性膜的微孔，具有更强的检测异质细胞混合物中各个细胞产物的能力。 通过使用多种检测颗粒，可以在单个孔中检测多种细胞产物。 此外，利用检测颗粒的固有光谱特性，不需要酶促反应来显现分泌的产物，从而增加灵敏度，重现性和易用性。

公开(公告)号：WO2005012505A2

公开(公告)日：2005-02-10

申请号：PCT/US2004/023582

申请日：2004-07-23

Applicant: CELLULAR TECHNOLOGY LTD. ,  LEHMANN, Paul, V. ,  KARULIN, Alexey, Y. ,  KLEEN, Thomas, Oliver

Inventor： LEHMANN, Paul, V. ,  KARULIN, Alexey, Y. ,  KLEEN, Thomas, Oliver

IPC: C12N5/00

CPC classification number: G01N33/505 ,  B82Y15/00 ,  G01N33/587 ,  G01N33/588 ,  G01N2333/5409 ,  G01N2333/57 ,  Y10T436/25375

Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.

Abstract translation: 本发明的实施方案涉及使用具有产品特异性检测试剂并具有特征光谱特征的检测粒子检测细胞产物的装置和方法。 特别地，提供了用于测量包括细胞因子在内的分泌的细胞产物的装置和方法。 描述了检测底物，包括其上具有产物特异性捕获试剂或包含疏水膜的微孔，其具有检测来自异种细胞混合物中的各个细胞的产物的更大能力。 通过使用多个检测粒子，可以在单个孔中检测多个细胞产物。 此外，使用检测颗粒的固有光谱性质，不需要酶反应来可视化分泌产物，从而增加灵敏度，再现性和易用性。

公开(公告)号：WO2012154215A1

公开(公告)日：2012-11-15

申请号：PCT/US2012/000232

申请日：2012-05-03

Applicant: CELLULAR TECHNOLOGY, LTD. ,  KUERTEN, Stefanie ,  LEHMANN, Paul, V.

Inventor： KUERTEN, Stefanie ,  LEHMANN, Paul, V.

IPC: A61K38/00

CPC classification number: G01N33/56966 ,  G01N33/6893 ,  G01N2333/54 ,  G01N2333/55 ,  G01N2333/57 ,  G01N2800/285 ,  G01N2800/50 ,  G01N2800/52 ,  G01N2800/54

Abstract: Embodiments of this invention include methods for detecting in vitro the presence in peripheral blood mononuclear cells (PBMCs), and in serum or plasma, of antibodies reactive to and of lymphocytes that are responsive to CNS antigens associated with Multiple Sclerosi (MS). These CNS antigens include, but are not limited to whole brain lysate and the myelin antigens myelin basic protein (MBP), myeli oligodendrocyte glycoprotein (MOG), MOG peptides (MOGps), proteolipid protein (PLP), and PLP peptides (PLPps). Stimulating PBMCs from patients with MS by CNS antigens cause B-lymphocytes to produce antibodies specific for CNS antigen, and causes T-lymphocytes to produce T-lymphocyte-specific cytokines, including interferon gamma (IFN-y), interteukin-2 (IL-2), or interleukin-17 (IL-17). In contrast, stimulating PBMCs from subjects without MS do not produce such responses.

Abstract translation: 本发明的实施方案包括在体外检测对与多重硬化（MS）相关的CNS抗原有反应性的抗体和淋巴细胞的抗体的外周血单核细胞（PBMC）和血清或血浆中的存在的方法。 这些CNS抗原包括但不限于全脑裂解物和髓鞘抗原髓鞘碱性蛋白（MBP），myeli少突胶质细胞糖蛋白（MOG），MOG肽（MOGPS），蛋白脂蛋白（PLP）和PLP肽（PLPps）。 通过CNS抗原刺激MS患者的PBMC，导致B淋巴细胞产生对CNS抗原特异性的抗体，并引起T淋巴细胞产生T淋巴细胞特异性细胞因子，包括干扰素γ（IFN-γ），白细胞介素-2（IL- 2）或白细胞介素-17（IL-17）。 相比之下，刺激来自没有MS的受试者的PBMC不产生这样的反应。

公开(公告)号：WO2006014925A2

公开(公告)日：2006-02-09

申请号：PCT/US2005/026435

申请日：2005-07-25

Applicant: CELLULAR TECHNOLOGY LTD. ,  LEHMANN, Paul, V. ,  KARULIN, Alexey, Y. ,  VELASCO, JR., Virgilio, B. ,  KLEEN, Thomas, Oliver

Inventor： LEHMANN, Paul, V. ,  KARULIN, Alexey, Y. ,  VELASCO, JR., Virgilio, B. ,  KLEEN, Thomas, Oliver

CPC classification number: H04N1/02815 ,  G01N21/253 ,  G01N21/255 ,  G01N21/6456 ,  H04N1/00795 ,  H04N1/00827 ,  H04N1/02845 ,  H04N1/1013

Abstract: Embodiments of this invention include flatbed scanners that have a surface and have a plurality of light sources. Alternatively, a single light source can be moved in two dimensions relative to an object placed on the surface of the scanner. Other embodiments include a scan head that is moveable in two dimensions relative to the flat bed of the scanner. Further embodiments include one or more polarizing filters to reduce glare and increase the quality of a captured image. Theses scanners can be used to capture images of enzyme linked immunosorbent assay (ELISA) substrates, plates having cultures thereon or other objects having irregular lower surfaces. Additional embodiments include methods for capturing images from ELISA substrates, bacterial culture plates, viral plaque assay plates and the like.

Abstract translation: 本发明的实施例包括具有表面并具有多个光源的平板扫描器。 或者，单个光源可以相对于放置在扫描仪表面上的物体在两个维度上移动。 其他实施例包括可相对于扫描仪的平板在两个维度上移动的扫描头。 另外的实施例包括一个或多个偏振滤光器以减少眩光并增加捕获图像的质量。 这些扫描仪可用于捕获酶联免疫吸附测定（ELISA）底物，其上具有培养物的培养皿或具有不规则下表面的其它物体的图像。 另外的实施方案包括从ELISA底物，细菌培养板，病毒斑块测定板等捕获图像的方法。