公开(公告)号：WO2005034206A3

公开(公告)日：2006-06-01

申请号：PCT/US2004005193

申请日：2004-02-20

Applicant: CALIFORNIA INST OF TECHN ,  WADE LAWRENCE A ,  SHAPIRO IAN R ,  COLLIER CHARLES PATRICK ,  ESPLANDIU MARIA J ,  BITTNER JR VERN GARRETT

Inventor： WADE LAWRENCE A ,  SHAPIRO IAN R ,  COLLIER CHARLES PATRICK ,  ESPLANDIU MARIA J ,  BITTNER JR VERN GARRETT

IPC: B05D3/00 ,  B82B3/00 ,  C12M1/00 ,  G01B5/00 ,  G01B7/00 ,  G01Q60/00 ,  G01Q70/12 ,  H01L20060101

CPC classification number: G01Q70/12

Abstract: Embodiments in accordance with the present invention relate to techniques for the growth and attachment of single wall carbon nanotubes (SWNT), facilitating their use as robust and well-characterized tools for AFM imaging and other applications. In accordance with one embodiment, SWNTs attached to an AFM tip can function as a structural scaffold for nanoscale device fabrication on a scanning probe. Such a probe can trigger, with nanometer precision, specific biochemical reactions or conformational changes in biological systems. The consequences of such triggering can be observed in real time by single-molecule fluorescence, electrical, and/or AFM sensing. Specific embodiments in accordance with the present invention utilize sensing and manipulation of individual molecules with carbon nanotubes, coupled with single-molecule fluorescence imaging, to allow observation of spectroscopic signals in response to mechanically induced molecular changes. Biological macromolecules such as proteins or DNA can be attached to nanotubes to create highly specific single-molecule probes for investigations of intermolecular dynamics, for assembling hybrid biological and nanoscale materials, or for developing molecular electronics. In one example, electrical wiring of single redox enzymes to carbon nanotube scanning probes allows observation and electrochemical control over single enzymatic reactions by monitoring fluorescence from a redox-active cofactor or the formation of fluorescent products. Enzymes "nanowired" to the tips of carbon nanotubes in accordance with embodiments of the present invention, may enable extremely sensitive probing of biological stimulus-response with high spatial resolution, including product-induced signal transduction.

Abstract translation: 根据本发明的实施方案涉及用于生长和附着单壁碳纳米管（SWNT）的技术，便于其用作用于AFM成像和其它应用的鲁棒且良好表征的工具。 根据一个实施例，附接到AFM尖端的SWNT可以用作扫描探针上的纳米级器件制造的结构支架。 这样的探针可以以纳米精度触发生物系统的特定生化反应或构象变化。 通过单分子荧光，电学和/或AFM感测可以实时观察到这种触发的后果。 根据本发明的具体实施方案利用与单分子荧光成像耦合的碳纳米管的单个分子的感测和操作，以便响应机械诱导的分子变化观察光谱信号。 可以将生物大分子如蛋白质或DNA连接到纳米管上，以产生用于分子间动力学研究的高度特异性单分子探针，用于组装混合生物和纳米级材料，或用于开发分子电子学。 在一个实例中，单个氧化还原酶与碳纳米管扫描探针的电气配线允许通过监测来自氧化还原活性辅助因子的荧光或荧光产物的形成来观察和电化学控制单个酶反应。 根据本发明的实施方案，“纳米线”针对碳纳米管的尖端的酶可以实现具有高空间分辨率（包括产物诱导的信号转导）的生物刺激反应的极度敏感的探测。

公开(公告)号：WO2007046819A3

公开(公告)日：2008-01-31

申请号：PCT/US2005041954

申请日：2005-11-15

Applicant: CALIFORNIA INSTITUE OF TECHNOL ,  WADE LAWARENCE A ,  COLLIER CHARLES PATRICK

Inventor： WADE LAWARENCE A ,  COLLIER CHARLES PATRICK

IPC: B05D1/18

CPC classification number: G01Q60/42 ,  G01Q80/00

Abstract: A device comprising an array of active regions for use in reacting one or more species in at least two of the active regions in a sequential process. The device has a transparent substrate member, which has a surface region and a silane material overlying the surface region; and a first active region formed overlying a first portion of the silane material. In an embodiment, the first active region has a first dimension of less than 1 micron in size and has one or more first molecules capable of binding to the first portion of the silane material. In an embodiment, the device has a second active region formed overlying a second portion of the silane material. The second active region has a second dimension of less than 1 micron in size and has one or more second molecules capable of binding to the second portion of the active region.

Abstract translation: 一种包括用于在顺序过程中使至少两个活性区域中的一种或多种物质反应的活性区域阵列的装置。 该装置具有透明基板部件，其具有覆盖表面区域的表面区域和硅烷材料; 以及形成为覆盖所述硅烷材料的第一部分的第一有源区。 在一个实施方案中，第一有源区具有尺寸小于1微米的第一尺寸，并具有一个或多个能够结合硅烷材料的第一部分的第一分子。 在一个实施例中，该装置具有形成在第二部分硅烷材料上的第二有源区。 第二有源区具有尺寸小于1微米的第二维度，并且具有一个或多个能够结合活性区的第二部分的第二分子。

公开(公告)号：WO2007046819A2

公开(公告)日：2007-04-26

申请号：PCT/US2005/041954

申请日：2005-11-15

Applicant: CALIFORNIA INSTITUE OF TECHNOLOGY ,  WADE, Lawarence, A. ,  COLLIER, Charles, Patrick

Inventor： WADE, Lawarence, A. ,  COLLIER, Charles, Patrick

IPC: C12Q1/68 ,  C12M1/34 ,  C12M3/00

CPC classification number: G01Q60/42 ,  G01Q80/00

Abstract: A device comprising an array of active regions for use in reacting one or more species in at least two of the active regions in a sequential process, e.g., sequential reactions. The device has a transparent substrate member, which has a surface region. The device has a silane material overlying the surface region. The device has a first active region formed overlying a first portion of the silane material. In a specific embodiment, the first active region has a first dimension of less than 1 micron in size and has one or more first molecules capable of binding to the first portion of the silane material. In a specific embodiment, the device has a second active region formed overlying a second portion of the silane material. The second active region has a second dimension of less than 1 micron in size and has one or more second molecules capable of binding to the second portion of the active region. In a specific embodiment, the device has a spatial distance separating the first active region and the second active region. In a preferred embodiment, the spatial distance is characterized by a dimension of 1 micron and less. The device has a fluid material in contact with the first active region, the second active region, and the spatial distance according to a specific embodiment. The device also has a reactant species within the fluid material. The reactant species is capable of spatially moving from the first active region to the second active region over the spatial distance of 1 micron and less within a time of less than 10 microseconds.

Abstract translation: 一种包含活性区域阵列的装置，其用于在顺序过程（例如顺序反应）中使至少两个活性区域中的一种或多种物质反应。 该装置具有透明基板部件，该基板部件具有表面区域。 该装置具有覆盖表面区域的硅烷材料。 该装置具有覆盖硅烷材料的第一部分的第一有源区。 在具体实施方案中，第一有源区具有尺寸小于1微米的第一尺寸，并具有一个或多个能够结合硅烷材料的第一部分的第一分子。 在具体实施方案中，该装置具有覆盖硅烷材料的第二部分的第二有源区。 第二有源区具有尺寸小于1微米的第二维度，并且具有一个或多个能够结合活性区的第二部分的第二分子。 在具体实施例中，该装置具有分隔第一有源区和第二有源区的空间距离。 在优选实施例中，空间距离的特征在于1微米和更小的尺寸。 根据具体实施例，该装置具有与第一有源区域，第二有源区域和空间距离接触的流体材料。 该装置还在流体材料内具有反应物种类。 反应物种能够在小于10微秒的时间内在空间距离为1微米以下的空间距离上从第一有源区域移动到第二有源区域。

公开(公告)号：WO2005076832A3

公开(公告)日：2007-09-20

申请号：PCT/US2005002668

申请日：2005-02-01

Applicant: CALIFORNIA INST OF TECHN ,  COLLIER CHARLES PATRICK ,  MA ZIYANG ,  QUAKE STEPHEN R ,  SHAPIRO IAN R ,  WADE LAWRENCE A

Inventor： COLLIER CHARLES PATRICK ,  MA ZIYANG ,  QUAKE STEPHEN R ,  SHAPIRO IAN R ,  WADE LAWRENCE A

IPC: G21K7/00 ,  C01B31/02 ,  G01Q10/00 ,  G01Q60/24 ,  G01Q60/38 ,  G01Q70/10 ,  G01Q70/12 ,  G01R3/00

CPC classification number: C01B32/162 ,  C01B2202/02 ,  C01B2202/34 ,  C01B2202/36 ,  G01Q60/38 ,  G01Q70/12 ,  G01R3/00

Abstract: A method for fabricating assembled structures. The method includes providing a tip structure, which has a first end, a second end, and a length defined between the first end and the second end. The second end is a free end. The method includes attaching a nano-sized structure along a portion of the length of the tip structure to extend a total length of the tip structure to include the length of the tip structure and a first length associated with the nano-sized structure. The method includes shortening the nano-sized structure from the first length to a second length. The method also includes pushing the nano-sized structure in a direction parallel to the second length to reduce the second length to a third length of the nano-sized structure along the direction parallel to the second length to cause the nano-sized structure to move along a portion of the length of the tip structure.

Abstract translation: 一种组装结构的制造方法。 该方法包括提供尖端结构，其具有第一端，第二端和限定在第一端和第二端之间的长度。 第二端是自由端。 该方法包括沿着尖端结构的长度的一部分附接纳米尺寸结构以延伸尖端结构的总长度以包括尖端结构的长度和与纳米尺寸结构相关联的第一长度。 该方法包括将纳米尺寸结构从第一长度缩短到第二长度。 该方法还包括沿平行于第二长度的方向推动纳米尺寸结构，以沿平行于第二长度的方向将纳米尺寸结构的第二长度减小到第三长度，以使纳米尺寸结构移动 沿着尖端结构的长度的一部分。

公开(公告)号：WO2005076832A2

公开(公告)日：2005-08-25

申请号：PCT/US2005/002668

申请日：2005-02-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  COLLIER, Charles, Patrick ,  MA, Ziyang ,  QUAKE, Stephen, R. ,  SHAPIRO, Ian, R. ,  WADE, Lawrence, A.

Inventor： COLLIER, Charles, Patrick ,  MA, Ziyang ,  QUAKE, Stephen, R. ,  SHAPIRO, Ian, R. ,  WADE, Lawrence, A.

IPC: C01B31/02 ,  G01Q10/00 ,  G01Q60/24 ,  G01Q60/38 ,  G01Q70/10 ,  G01Q70/12 ,  G01R3/00 ,  G21K7/00

CPC classification number: C01B32/162 ,  C01B2202/02 ,  C01B2202/34 ,  C01B2202/36 ,  G01Q60/38 ,  G01Q70/12 ,  G01R3/00

Abstract: A method for fabricating assembled structures. The method includes providing a tip structure, which has a first end, a second end, and a length defined between the first end and the second end. The second end is a free end. The method includes attaching a nano-sized structure along a portion of the length of the tip structure to extend a total length of the tip structure to include the length of the tip structure and a first length associated with the nano-sized structure. The method includes shortening the nano-sized structure from the first length to a second length. The method also includes pushing the nano-sized structure in a direction parallel to the second length to reduce the second length to a third length of the nano-sized structure along the direction parallel to the second length to cause the nano-sized structure to move along a portion of the length of the tip structure.

Abstract translation: 一种组装结构的制造方法。 该方法包括提供尖端结构，其具有第一端，第二端和限定在第一端和第二端之间的长度。 第二端是自由端。 该方法包括沿着尖端结构的长度的一部分附接纳米尺寸结构以延伸尖端结构的总长度以包括尖端结构的长度和与纳米尺寸结构相关联的第一长度。 该方法包括将纳米尺寸结构从第一长度缩短到第二长度。 该方法还包括沿平行于第二长度的方向推动纳米尺寸结构，以沿平行于第二长度的方向将纳米尺寸结构的第二长度减小到第三长度，以使纳米尺寸结构移动 沿着尖端结构的长度的一部分。

公开(公告)号：WO2005034206A2

公开(公告)日：2005-04-14

申请号：PCT/US2004/005193

申请日：2004-02-20

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  WADE, Lawrence, A. ,  SHAPIRO, Ian, R. ,  COLLIER, Charles, Patrick ,  ESPLANDIU, Maria, J. ,  BITTNER, JR., Vern, Garrett

Inventor： WADE, Lawrence, A. ,  SHAPIRO, Ian, R. ,  COLLIER, Charles, Patrick ,  ESPLANDIU, Maria, J. ,  BITTNER, JR., Vern, Garrett

IPC: H01L

CPC classification number: G01Q70/12

Abstract: Embodiments in accordance with the present invention relate to techniques for the growth and attachment of single wall carbon nanotubes (SWNT), facilitating their use as robust and well-characterized tools for AFM imaging and other applications. In accordance with one embodiment, SWNTs attached to an AFM tip can function as a structural scaffold for nanoscale device fabrication on a scanning probe. Such a probe can trigger, with nanometer precision, specific biochemical reactions or conformational changes in biological systems. The consequences of such triggering can be observed in real time by single-molecule fluorescence, electrical, and/or AFM sensing. Specific embodiments in accordance with the present invention utilize sensing and manipulation of individual molecules with carbon nanotubes, coupled with single-molecule fluorescence imaging, to allow observation of spectroscopic signals in response to mechanically induced molecular changes. Biological macromolecules such as proteins or DNA can be attached to nanotubes to create highly specific single-molecule probes for investigations of intermolecular dynamics, for assembling hybrid biological and nanoscale materials, or for developing molecular electronics. In one example, electrical wiring of single redox enzymes to carbon nanotube scanning probes allows observation and electrochemical control over single enzymatic reactions by monitoring fluorescence from a redox-active cofactor or the formation of fluorescent products. Enzymes "nanowired" to the tips of carbon nanotubes in accordance with embodiments of the present invention, may enable extremely sensitive probing of biological stimulus-response with high spatial resolution, including product-induced signal transduction.

Abstract translation: 根据本发明的实施方案涉及用于生长和附着单壁碳纳米管（SWNT）的技术，便于将其用作用于AFM成像和其它应用的鲁棒且良好表征的工具。 根据一个实施例，附接到AFM尖端的SWNT可以用作扫描探针上的纳米级器件制造的结构支架。 这样的探针可以以纳米精度触发生物系统中的特定生化反应或构象变化。 这种触发的后果可以通过单分子荧光，电学和/或AFM感测实时观察到。 根据本发明的具体实施方案利用与单分子荧光成像耦合的碳纳米管的单个分子的感测和操纵，以便响应于机械诱导的分子变化观察光谱信号。 生物大分子如蛋白质或DNA可以连接到纳米管上，以产生用于分子间动力学研究的高度特异性单分子探针，用于组装混合生物和纳米级材料，或用于开发分子电子学。 在一个实例中，单个氧化还原酶与碳纳米管扫描探针的电气配线允许通过监测来自氧化还原活性辅助因子的荧光或荧光产物的形成来观察和电化学控制单个酶反应。 根据本发明的实施方案，“纳米线”纳米线对碳纳米管的尖端可以实现具有高空间分辨率（包括产物诱导的信号转导）的生物刺激反应的极度敏感的探测。