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公开(公告)号：WO2010129577A3
公开(公告)日：2010-11-11
申请号：PCT/US2010/033584
申请日：2010-05-04
Applicant: INTERGRATED NANO-TECHNOLOGIES, INC. , CONNOLLY, Dennis, M. , DEBOER, Charles , TANNOUS, Vera
Inventor： CONNOLLY, Dennis, M. , DEBOER, Charles , TANNOUS, Vera
Abstract: A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range.
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公开(公告)号：WO2005065290A3
公开(公告)日：2005-11-03
申请号：PCT/US2004043530
申请日：2004-12-22
Applicant: INTEGRATED NANO TECH LLC , CONNOLLY DENNIS M , DEBOER CHARLES
Inventor： CONNOLLY DENNIS M , DEBOER CHARLES
Abstract: The present invention relates to a method for metallizing a nucleic acid molecule by providing a metal sol, mordanting metal particles from the metal sol on the nucleic acid molecule, and contacting the mordanted nucleic acid molecule with a metal deposition solution to deposit metal on the nucleic acid molecule. A method for detecting a target nucleic acid molecule in a sample employing metallized nucleic acid molecules is also disclosed.
Abstract translation: 本发明涉及通过提供金属溶胶，金属溶胶在核酸分子上媒染金属颗粒并使该发光核酸分子与金属沉积溶液接触以将金属沉积在核酸上的金属化方法， 酸分子。 还公开了使用金属化核酸分子检测样品中的靶核酸分子的方法。
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公开(公告)号：WO2004096986A3
公开(公告)日：2005-03-24
申请号：PCT/US2004012161
申请日：2004-04-20
Applicant: INTEGRATED NANO TECH LLC , CONNOLLY DENNIS M , DEBOER CHARLES D
Inventor： CONNOLLY DENNIS M , DEBOER CHARLES D
IPC: C12N20060101 , C12Q1/68
CPC classification number: C12Q1/6825 , C12Q2565/519 , C12Q2565/607 , C12Q2565/501
Abstract: The present invention is directed to a method of quantitatively detecting target nucleic acid molecules in a sample. In carrying out this method, one or more different groups of two or more electrically separated electrical conductors are provided with capture probes attached to the conductors such that a gap exists between the capture probes on the electrically separated conductors. The capture probes are contacted with a sample, potentially containing the target nucleic acid molecules, under conditions effective to permit any of the target nucleic acid molecule present in the sample to hybridize to the capture probes and thereby connect the capture probes. The presence of the target nucleic acid molecules is detected by determining whether electricity is conducted between the electrically separated conductors. The electrically separated conductors are then interrogated to quantify the concentration of target nucleic acid molecules present in the sample.
Abstract translation: 本发明涉及定量检测样品中靶核酸分子的方法。 在实施该方法时，具有两个或更多个电分离的电导体的一个或多个不同的组，其中附接到导体的捕获探针使得在电分离的导体上的捕获探针之间存在间隙。 捕获探针在有效使得样品中存在的任何目标核酸分子与捕获探针杂交从而连接捕获探针的条件下，与可能含有靶核酸分子的样品接触。 通过确定在电分离的导体之间是否传导电流来检测目标核酸分子的存在。 然后询问电分离的导体以定量样品中存在的靶核酸分子的浓度。
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公开(公告)号：WO2005019413A2
公开(公告)日：2005-03-03
申请号：PCT/US2004/018374
申请日：2004-06-09
Inventor： CONNOLLY, Dennis, M. , CHAFIN, David, R.
IPC: C12N
CPC classification number: C12Q1/6825 , C12Q2527/125
Abstract: The present invention relates to a device for the detection of a target molecule in an environment. The device includes a detection system having a detection cartridge containing a detection chip where capture probes are attached to two or more electrically separated conductors such that a gap exists between the capture probes on the electrically separated conductors. A sample can be analyzed for the presence of the target molecule by determining whether any target molecules present in the sample bind to the capture probes and electrically connect the conductors. The device includes a cartridge manipulator having a plurality of detection cartridges where an unused individual detection cartridge can be inserted into a support unit for detection of the target molecule in the sample. The present invention also relates to a method for the detection of a biological agent using the device disclosed.
Abstract translation: 本发明涉及用于在环境中检测靶分子的装置。 该装置包括具有检测盒（303）的检测系统，检测盒（303）包含检测芯片，其中捕获探针附接到两个或更多个电分离的导体，使得在电分离的导体上的捕获探针之间存在间隙。 可以通过确定样品中存在的任何靶分子是否与捕获探针结合并电连接导体，来分析样品是否存在目标分子。 该装置包括具有多个检测盒（303）的盒式操纵器（302），其中未使用的单独检测盒可以插入到用于检测样品中的目标分子的支撑单元（300）中。 本发明还涉及使用所公开的装置检测生物试剂的方法。
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5.发明申请
公开(公告)号：WO2004042344A3
公开(公告)日：2004-12-16
申请号：PCT/US0236650
申请日：2002-11-14
Applicant: INTEGRATED NANO TECH LLC , PHAM HONG-AN , CONNOLLY DENNIS M
Inventor： PHAM HONG-AN , CONNOLLY DENNIS M
IPC: C07H21/02 , C07H21/04 , C12Q1/68 , C25D3/04 , C25D3/48 , C25D5/00 , C40B40/06 , G01N20060101 , G01N33/543 , G03C5/00
CPC classification number: G01N33/5438 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , B01J2219/0074 , C40B40/06
Abstract: The present invention relates to a method of manufacturing a detection device which involves providing a substrate having a layer of gold and a first layer of photosensitive material. Next, the substrate is subjected to a first level photolithography treatment to produce a gold electrical conductor containing conductive fingers with spaces between them. Finally, biological probes are attached to the conductive fingers under conditions effective to form a gap between the biological probes on the spaced apart conductive fingers. As a result, a target molecule, if present in a sample, can bind to a pair of the biological probes on the spaced apart conductive fingers to bridge the gap between the biological probes, allowing detection of the target molecule.
Abstract translation: 本发明涉及一种制造检测装置的方法，该方法包括提供具有金层和第一层光敏材料的基片。 接下来，对衬底进行第一级光刻处理以产生含有导电指的金电导体，其间具有空间。 最后，在有效地在间隔开的导电指状物上的生物探针之间形成间隙的条件下，将生物探针连接到导电指状物上。 结果，目标分子（如果存在于样品中）可以与间隔开的导电指状物上的一对生物探针结合以桥接生物探针之间的间隙，从而允许检测目标分子。
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Patent Agency Ranking6.发明申请
公开(公告)号：WO2003057901A3
公开(公告)日：2003-07-17
申请号：PCT/US2003/000268
申请日：2003-01-06
Applicant: INTEGRATED NANO-TECHNOLOGIES, LLC , CONNOLLY, Dennis, M. , DEBOER, Charles, D. , POTTER, Michael, D.
Inventor： CONNOLLY, Dennis, M. , DEBOER, Charles, D. , POTTER, Michael, D.
IPC: C12Q1/68
Abstract: The present invention relates to methods for detecting a target nucleic acid molecule in a sample. The methods involve providing a device having surfaces not in contact with each other, at least one of which is moveable. A pair of oligonucleotide probes complementary to the target nucleic acid molecule are attached to the surfaces of the device. The device is then contacted with a sample, under selective hybridization conditions, to permit target molecules, if any, present in the sample, to hybridize to the probes. Finally, it is determined whether there is a decrease in the relative movement between the surfaces, thereby indicating the presence of the target molecule in the sample. Alternatively, single stranded nucleic acid molecules are attached to the surfaces to determine whether the two surfaces are closer to one another than prior to the contacting. Devices used to carry out the above methods are also disclosed.
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公开(公告)号：WO2010129577A2
公开(公告)日：2010-11-11
申请号：PCT/US2010033584
申请日：2010-05-04
Applicant: INTERGRATED NANO TECHNOLOGIES , CONNOLLY DENNIS M , DEBOER CHARLES , TANNOUS VERA
Inventor： CONNOLLY DENNIS M , DEBOER CHARLES , TANNOUS VERA
CPC classification number: C12N15/1003
Abstract: A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range.
Abstract translation: 一种通过在尺寸稳定剂存在下利用机械力来制备样品以分离样品以获得可用尺寸范围的核酸分子的方法。
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公开(公告)号：WO2010115192A3
公开(公告)日：2010-10-07
申请号：PCT/US2010/029961
申请日：2010-04-05
Applicant: INTEGRATED NANO-TECHNOLOGIES, INC. , KILCOIN, Christopher , APTEKAREV, Konstantin , MURANTE, Richard, S. , CONNOLLY, Dennis, M.
Inventor： KILCOIN, Christopher , APTEKAREV, Konstantin , MURANTE, Richard, S. , CONNOLLY, Dennis, M.
Abstract: A fluid delivery system having a rotating reservoir insert and a cartridge body. The rotating reservoir insert having a plurality of reservoirs in communication with ports on the external surface of the insert. The ports are positioned such that upon rotation the port is in-line with a fluid extracting device, such as a syringe, capable of extracting fluid from the reservoir.
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公开(公告)号：WO2005019413A3
公开(公告)日：2005-04-07
申请号：PCT/US2004018374
申请日：2004-06-09
Applicant: INTEGRATED NANO TECH LLC , CONNOLLY DENNIS M , CHAFIN DAVID R
Inventor： CONNOLLY DENNIS M , CHAFIN DAVID R
IPC: C12M1/34 , C12N20060101 , C12Q1/68
CPC classification number: C12Q1/6825 , C12Q2527/125
Abstract: The present invention relates to a device for the detection of a target molecule in an environment. The device includes a detection system having a detection cartridge (303) containing a detection chip where capture probes are attached to two or more electrically separated conductors such that a gap exists between the capture probes on the electrically separated conductors. A sample can be analyzed for the presence of the target molecule by determining whether any target molecules present in the sample bind to the capture probes and electrically connect the conductors. The device includes a cartridge manipulator (302) having a plurality of detection cartridges (303) where an unused individual detection cartridge can be inserted into a support unit (300) for detection of the target molecule in the sample. The present invention also relates to a method for the detection of a biological agent using the device disclosed.
Abstract translation: 本发明涉及用于检测环境中的靶分子的装置。 该装置包括检测系统，该检测系统具有包含检测芯片的检测盒（303），其中捕获探针附接到两个或更多个电分离的导体，使得在电分离的导体上的捕获探针之间存在间隙。 通过确定样品中存在的任何靶分子是否与捕获探针结合并电连接导体，可以分析样品中目标分子的存在。 该装置包括具有多个检测盒（303）的盒操纵器（302），其中未使用的个体检测盒可以插入到用于检测样本中的目标分子的支撑单元（300）中。 本发明还涉及使用所公开的装置检测生物因子的方法。
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公开(公告)号：WO2004096986A2
公开(公告)日：2004-11-11
申请号：PCT/US2004/012161
申请日：2004-04-20
Inventor： CONNOLLY, Dennis, M. , DEBOER, Charles, D.
IPC: C12N
CPC classification number: C12Q1/6825 , C12Q2565/519 , C12Q2565/607 , C12Q2565/501
Abstract: The present invention is directed to a method of quantitatively detecting target nucleic acid molecules in a sample. In carrying out this method, one or more different groups of two or more electrically separated electrical conductors are provided with capture probes attached to the conductors such that a gap exists between the capture probes on the electrically separated conductors. The capture probes are contacted with a sample, potentially containing the target nucleic acid molecules, under conditions effective to permit any of the target nucleic acid molecule present in the sample to hybridize to the capture probes and thereby connect the capture probes. The presence of the target nucleic acid molecules is detected by determining whether electricity is conducted between the electrically separated conductors. The electrically separated conductors are then interrogated to quantify the concentration of target nucleic acid molecules present in the sample.
Abstract translation: 本发明涉及定量检测样品中靶核酸分子的方法。 在实施该方法时，具有两个或更多个电分离的电导体的一个或多个不同的组，其中附接到导体的捕获探针使得在电分离的导体上的捕获探针之间存在间隙。 在有效使得样品中存在的任何目标核酸分子与捕获探针杂交从而连接捕获探针的条件下，将捕获探针与可能含有靶核酸分子的样品接触。 通过确定在电分离的导体之间是否导通电流来检测目标核酸分子的存在。 然后询问电分离的导体以定量样品中存在的靶核酸分子的浓度。
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