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1.发明申请
公开(公告)号：WO2023088991A1
公开(公告)日：2023-05-25
申请号：PCT/EP2022/082182
申请日：2022-11-17
申请人： IST INNUSCREEN GMBH
发明人： HILLEBRAND, Timo , GRASER, Elmara , JACOBI, Wiebke
IPC分类号： C12N15/10
摘要： Die Erfindung betrifft ein Verfahren zur Erzeugung einer mindestens eine Zielsubstanz enthaltenden Probe aus einem ersten Flüssigkeitsvolumen (1) einer die mindestens eine Zielsubstanz enthaltenden Probeflüssigkeit durch Aufkonzentrierung der Zielsubstanz in dem ersten Flüssigkeitsvolumen (1), umfassend: - Zugeben eines Superabsorbers (2) zu dem ersten Flüssigkeitsvolumen (1) oder Zugeben des Flüssigkeitsvolumens (1) zu dem Superabsorber (2), - Inkubieren des aus dem Superabsorber (2) und dem ersten Flüssigkeitsvolumen (1) gebildeten ersten Gemisches, und - Entnehmen einer Probe des nach dem Inkubieren vorliegenden flüssigen Anteils (3) des ersten Gemisches. Die Erfindung betrifft auch ein Verfahren zur Erzeugung einer mindestens eine Zielsubstanz enthaltenden Probe aus einem ersten Flüssigkeitsvolumen (1) einer die mindestens eine Zielsubstanz enthaltenden Probeflüssigkeit, umfassend: - Zugeben eines Superabsorbers (2) zu dem ersten Flüssigkeitsvolumen (1) oder Zugeben des ersten Flüssigkeitsvolumens (1) zu dem Superabsorber (2), - Inkubieren des aus dem Superabsorber (2) und dem ersten Flüssigkeitsvolumen (1) gebildeten ersten Gemisches, - danach Zugeben eines zweiten Flüssigkeitsvolumens einer wässrigen Lösung zum verbliebenen Superabsorber, - Inkubieren des aus dem Superabsorber und dem zweiten Flüssigkeitsvolumen gebildeten zweiten Gemisches, - Entnehmen einer Probe des nach dem Inkubieren vorliegenden flüssigen Anteils des zweiten Gemisches.
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公开(公告)号：WO2023085955A1
公开(公告)日：2023-05-19
申请号：PCT/NZ2022/050140
申请日：2022-11-09
IPC分类号： C12N15/10 , C12N15/70 , C12N9/00 , C12Q1/25 , C12Q1/6806
摘要： The present disclosure encompasses RNA ligase enzymes, particularly thermostable RNA ligase enzymes, and including variant enzymes, along with the preparation and use thereof. Specifically encompassed are methods for preparing the RNA ligase enzymes, methods for ligation using these enzymes, as well as polynucleotides used for producing these enzymes, libraries comprising these polynucleotides, and microbial strains comprising these polynucleotides.
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公开(公告)号：WO2023084369A1
公开(公告)日：2023-05-19
申请号：PCT/IB2022/060640
申请日：2022-11-04
发明人： WATTS, Annabelle , BONILLA, Tonya D. , RASMUSSEN, Jerald K. , FISHMAN, Joshua M. , TRAEGER, Lindsay L. , COLAK ATAN, Semra
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6813
摘要： A method of processing polynucleic acids is described comprising: providing a solid support comprising ligands with an amide group; exposing the solid support to polynucleic acid molecules in a buffer having a pH less than 5.5 to bind at least a portion of the polynucleic acid molecules to the ligands; c) exposing the solid support (e.g. particles) with bound polynucleic acid molecules to a buffer having a pH greater than 6 to release a portion of the bound polynucleic acid molecules from the ligands of the particles and to retain a portion of the polynucleic acid molecules bound to the solid support (e.g. particles); and utilizing the solid support with retained bound polynucleic acid molecules or suspension thereof.
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公开(公告)号：WO2023081796A1
公开(公告)日：2023-05-11
申请号：PCT/US2022/079270
申请日：2022-11-04
IPC分类号： C12Q1/6886 , C12N15/10 , C12Q1/6806
摘要： The present disclosure relates to detecting one or more types of oropharyngeal cancer in a biological sample from a subject. In particular, the present disclosure provides compositions and methods for detecting the presence or absence of one or more types of oropharyngeal cancer (e.g., HPV+ oropharyngeal squamous cell cancer) in a biological sample from a subject having or suspected of having an oropharyngeal cancer.
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公开(公告)号：WO2023081756A1
公开(公告)日：2023-05-11
申请号：PCT/US2022/079220
申请日：2022-11-03
申请人： THE J. DAVID GLADSTONE INSTITUTES, A TESTAMENTARY TRUST ESTABLISHED UNDER THE WILL OF J. DAVID GLADSTONE , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , LOPEZ, Santiago C. , SHIPMAN, Seth
发明人： LOPEZ, Santiago C. , SHIPMAN, Seth
IPC分类号： C12N15/113 , C12N15/10 , C12N9/12 , C12N9/22 , A61K31/7088
摘要： Described herein are compositions and methods that provide precise editing of cells, including mammalian cells and human cells. The compositions and methods utilize retrons as repair donors, retron reverse transcriptases to make those retron repair donors, retron-encoded guide RNAs, and CRISPR nucleases.
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公开(公告)号：WO2023075388A1
公开(公告)日：2023-05-04
申请号：PCT/KR2022/016412
申请日：2022-10-26
申请人： (주)한국첨단시험연구원
IPC分类号： C12N15/10 , C12Q1/6844
摘要： 본 발명은 미생물 DNA 순수분리를 저해하는 물질들을 효과적으로 제거하는 침전제 조성물 및 이것이 적용된 고농도, 고순도 DNA 추출방법에 관한 것으로서, 보다 상세하게는 시료 중의 세포를 용해하여 이로부터 DNA를 추출하는 과정에서 DNA 순수분리를 저해하는 불순물들을 침전시켜 제거하기 위한 용도로 사용되는 것으로서, 1.3~2.1 %(w/v) Phosphotungstic acid hydrate, 1.2~1.8 %(w/v) Zinc acetate dihydrate, 13.0~19.0 %(v/v) Acetic acid 및 나머지가 물인 것을 특징으로 하는 불순물 침전제 조성물, 상기 침전제 조성물이 적용된 DNA 추출방법 및 이렇게 추출된 DNA의 증폭방법에 관한 것이다.
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公开(公告)号：WO2023070003A1
公开(公告)日：2023-04-27
申请号：PCT/US2022/078388
申请日：2022-10-19
IPC分类号： A61K31/713 , A61P1/16 , A61P11/00 , C12N9/22 , C12N15/10 , C12N15/113 , C12N15/90
摘要： Disclosed are engineered meganucleases that bind and cleave a recognition sequence within a serine peptidase inhibitor, Clade A, Member 1 (SERPINA1) gene, which encodes alpha-1 antitrypsin (AAT). Further disclosed are donor polynucleotides that encode functional AAT proteins. The present disclosure also encompasses methods of using such engineered meganucleases and donor polynucleotides to make genetically-modified cells and use of such compositions for treatment of AAT deficiency.
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公开(公告)号：WO2023069972A1
公开(公告)日：2023-04-27
申请号：PCT/US2022/078337
申请日：2022-10-19
摘要： Genome editing tools for use in systems designed to deliver large genetic elements are disclosed herein. A genome editing system is described, which includes i) an R2 element enzyme or other non-LTR site specific retrotransposon element and ii) a payload RNA, wherein the payload RNA comprises an insertion region and optionally one or more of a 5' homology region, a 3' homology region, and a protein binding element, wherein the insertion region comprises a template for a small or large nucleic acid insertion into the genome, and wherein the R2 element enzyme or other non-LTR site specific retrotransposon element comprises a targeting domain, a reverse transcriptase domain, and a nickase domain. Also disclosed are cells edited using such a genome editing system, methods for editing a genome, and compositions comprising cells edited with this genomic editing system.
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公开(公告)号：WO2023067577A1
公开(公告)日：2023-04-27
申请号：PCT/IB2022/060159
申请日：2022-10-21
发明人： HIRANO, Naoto , MURATA, Kenji , LY, Dalam , SAIJO, Hiroshi , MATSUNAGA, Yukiko
IPC分类号： C07K14/74 , A61K31/7088 , A61K38/17 , A61K38/46 , A61P35/00 , A61P37/02 , C12N15/10 , C12N15/12
摘要： The present disclosure is directed to methods of modifying an HLA-binding pocket in an HLA molecule in a subject. Some aspects are directed to HLA molecules comprising a modified HLA-binding pocket, where the HLA molecule has increased affinity for a peptide, e.g., an antigen. Other aspects are directed to compositions comprising the same and methods of using the same.
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公开(公告)号：WO2023066735A1
公开(公告)日：2023-04-27
申请号：PCT/EP2022/078295
申请日：2022-10-11
摘要： Use of one or more inhibitor(s) of senescence for increasing the survival and/or engraftment of haematopoietic cells, haematopoietic stem cells, haematopoietic progenitor cells and/or T cells in gene therapy.
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