公开(公告)号：WO2011021194A3

公开(公告)日：2011-06-03

申请号：PCT/IL2010000669

申请日：2010-08-17

Applicant: TECHNION RES & DEV FOUNDATION ,  DAR-OAKNIN AYELET ,  ITSKOVITZ-ELDOR JOSEPH

Inventor： DAR-OAKNIN AYELET ,  ITSKOVITZ-ELDOR JOSEPH

IPC: C12N5/0797

CPC classification number: C12N5/0692 ,  A61K35/44 ,  A61K35/545 ,  C12N5/0603 ,  C12N2506/02 ,  C12N2506/45

Abstract: Provided are methods of isolating pericyte progenitor cells from pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells, by isolating CD105+, CD73+ and/or CD105+/CD73+ cells from embryoid bodies and optionally by enriching the cells with CD31- cells. Also provided are methods of isolating endothelial cells and co-derivation of pericyte and endothelial cells progenitor cells from embryoid bodies, and methods of differentiating same for various therapeutic applications. In addition, the invention provides an isolated pericyte progenitor cell having an expression marker signature of CD105+/CD73+CD31-/alpha SMA-/CD133-/Flk-1-.

Abstract translation: 提供了通过从胚状体分离CD105 +，CD73 +和/或CD105 + / CD73 +细胞并任选地通过用CD31-细胞富集细胞从多能干细胞例如胚胎干细胞和诱导的多能干细胞分离周细胞祖细胞的方法。 还提供了分离内皮细胞和从胚状体共同衍生周细胞和内皮细胞祖细胞的方法，以及用于各种治疗应用的差异化方法。 此外，本发明提供了具有CD105 + / CD73 + CD31- /αSMA- / CD133- / Flk-1-的表达标记特征的分离的周细胞祖细胞。

公开(公告)号：WO2011021194A2

公开(公告)日：2011-02-24

申请号：PCT/IL2010/000669

申请日：2010-08-17

Applicant: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LTD. ,  DAR-OAKNIN, Ayelet ,  ITSKOVITZ-ELDOR, Joseph

Inventor： DAR-OAKNIN, Ayelet ,  ITSKOVITZ-ELDOR, Joseph

IPC: C12N5/0797

CPC classification number: C12N5/0692 ,  A61K35/44 ,  A61K35/545 ,  C12N5/0603 ,  C12N2506/02 ,  C12N2506/45

Abstract: Provided are methods of isolating pericyte progenitor cells from pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells, by isolating CD105+, CD73+ and/or CD105+/CD73+ cells from embryoid bodies and optionally by enriching the cells with CD31- cells. Also provided are methods of isolating endothelial cells and co-derivation of pericyte and endothelial cells progenitor cells from embryoid bodies, and methods of differentiating same for various therapeutic applications. In addition, the invention provides an isolated pericyte progenitor cell having an expression marker signature of CD105+/CD73+CD31-/alpha SMA-/CD133-/Flk-1-.

Abstract translation: 提供了通过从胚状体分离CD105 +，CD73 +和/或CD105 + / CD73 +细胞并且任选通过富集从多能干细胞例如胚胎干细胞和诱导性多能干细胞分离周细胞祖细胞的方法 具有CD31-细胞的细胞。 还提供了分离内皮细胞和从胚状体共体衍生周细胞和内皮细胞祖细胞的方法，以及将其用于各种治疗应用的方法。 此外，本发明提供了具有CD105 + / CD73 + CD31- /αSMA-/ CD133- / Flk-1 - 表达标记特征的分离的周细胞祖细胞。