公开(公告)号：WO2010054000A1

公开(公告)日：2010-05-14

申请号：PCT/US2009/063292

申请日：2009-11-04

Applicant: JOSEPH, Richard ,  DIMEO, James ,  JAROSZ, Mirna ,  THOMPSON, John, F. ,  BOWERS, Jayson ,  CHOUINARD, Scott ,  KAPRANOV, Philipp ,  EFCAVITCH, William, J. ,  HART, Christopher ,  OZSOLAK, Faith

Inventor： JOSEPH, Richard ,  DIMEO, James ,  JAROSZ, Mirna ,  THOMPSON, John, F. ,  BOWERS, Jayson ,  CHOUINARD, Scott ,  KAPRANOV, Philipp ,  EFCAVITCH, William, J. ,  HART, Christopher ,  OZSOLAK, Faith

IPC: C12M1/00

CPC classification number: B01L3/502715 ,  B01L3/5025 ,  B01L2200/028 ,  B01L2300/0877 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0445 ,  B01L2400/0487 ,  C12Q1/6869 ,  Y10T436/143333

Abstract: A method, flow cell and/or device for increasing the recovery of a limiting analyte in a sample, e.g., for single molecule analysis is disclosed. Methods for preparing a nucleic acid sample from a single cell and capturing nucleic acids on a surface configured for use in or with single molecule analysis are also provided.

Abstract translation: 公开了用于增加样品中限制性分析物的回收的方法，流动池和/或装置，例如用于单分子分析。 还提供了从单个细胞制备核酸样品并捕获构建用于单分子分析或用于单分子分析的表面上的核酸的方法。

公开(公告)号：WO2007100637A2

公开(公告)日：2007-09-07

申请号：PCT/US2007/004679

申请日：2007-02-21

Applicant: HELICOS BIOSCIENCES CORPORATION ,  EFCAVITCH, William, J. ,  CAUSEY, Marie, Sutherlin

Inventor： EFCAVITCH, William, J. ,  CAUSEY, Marie, Sutherlin

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2565/519 ,  C12Q2533/101 ,  C12Q2527/125

Abstract: The invention relates to methods for detecting a mutation in a nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease.

Abstract translation: 本发明涉及用于检测核酸中的突变的方法。 本发明的方法可用于检测和鉴定指示疾病或疾病倾向的突变。

公开(公告)号：WO2010051434A1

公开(公告)日：2010-05-06

申请号：PCT/US2009/062732

申请日：2009-10-30

Applicant: HELICOS BIOSCIENCES CORPORATION ,  EFCAVITCH, William, J. ,  THOMPSON, John, F. ,  BUZBY, Philip, R. ,  BOWERS, Jayson, L.

Inventor： EFCAVITCH, William, J. ,  THOMPSON, John, F. ,  BUZBY, Philip, R. ,  BOWERS, Jayson, L.

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N9/1241 ,  C12Q1/6869

Abstract: The invention generally relates to polymerases for efficient and controlled sequencing-by-synthesis reactions. In certain embodiments, the invention provides a polymerase enzyme including at least one mutation that enhances ability of the polymerase as compared to a wild-type polymerase to incorporate a nucleotide into a nascent strand of DNA or cDNA including at least one modified nucleotide.

Abstract translation: 本发明一般涉及用于有效和控制的合成反应的聚合酶。 在某些实施方案中，本发明提供了聚合酶，其包括与野生型聚合酶相比增强聚合酶能力的至少一个突变，以将核苷酸掺入包含至少一个修饰的核苷酸的DNA或cDNA的新生链中。

公开(公告)号：WO2007100637A3

公开(公告)日：2007-11-15

申请号：PCT/US2007004679

申请日：2007-02-21

Applicant: HELICOS BIOSCIENCES CORP ,  EFCAVITCH WILLIAM J ,  CAUSEY MARIE SUTHERLIN

Inventor： EFCAVITCH WILLIAM J ,  CAUSEY MARIE SUTHERLIN

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2565/519 ,  C12Q2533/101 ,  C12Q2527/125

Abstract: The invention relates to methods for detecting a mutation in a nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease.

Abstract translation: 本发明涉及检测核酸突变的方法。 本发明的方法可用于检测和鉴定指示疾病或疾病倾向的突变。