利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

1992 条记录 (耗时 0.03 秒)

    DISSOLUTION TEST FOR SENSITIVE DRUGS
    1.
    发明申请
    DISSOLUTION TEST FOR SENSITIVE DRUGS 审中-公开

    公开(公告)号：WO2019082169A1

    公开(公告)日：2019-05-02

    申请号：PCT/IB2018/058415

    申请日：2018-10-27

    申请人： GOURABI, Hamid ,  MOBEDI, Hamid ,  MIRZAEI, Siyavash ,  KHEZLI, Sakineh

    发明人： GOURABI, Hamid ,  MOBEDI, Hamid ,  MIRZAEI, Siyavash ,  KHEZLI, Sakineh

    IPC分类号： G01N33/15

    CPC分类号： G01N33/15 ,  B01L7/02 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2400/0457 ,  B01L2400/0487

    摘要： A method and apparatus for drug dissolution testing may include an overflow vessel with an overflow outlet port at a predetermined height from a bottom of the overflow vessel, a first water bath submerging the overflow vessel and configured to keep a temperature of the overflow vessel at a first predetermined temperature, a pressurized vessel containing a dissolution medium, a second water bath submerging the pressurized vessel and configured to keep the dissolution medium at a second predetermined temperature, a dissolution medium path with an output end connected in fluid communication with the flow cell and an input end attached in fluid communication to the pressurized vessel, where the dissolution medium path may transfer the dissolution medium from the pressurized vessel into the flow cell, a collection vessel connected to the overflow outlet port of the flow cell, and a third water bath submerging the collection vessel and configured to keep the collection vessel at a third predetermined temperature.

    SYSTEM AND METHOD FOR DETERMINING MECHANICAL PROPERTIES OF BIOLOGICAL CELLS
    3.
    发明申请
    SYSTEM AND METHOD FOR DETERMINING MECHANICAL PROPERTIES OF BIOLOGICAL CELLS 审中-公开

    公开(公告)号：WO2018207087A1

    公开(公告)日：2018-11-15

    申请号：PCT/IB2018/053181

    申请日：2018-05-08

    申请人： INDIAN INSTITUTE OF SCIENCE

    发明人： KOTESA, Rahul Singh ,  SEN, Prosenjit

    IPC分类号： G01N33/48

    CPC分类号： G01N33/5026 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0663 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2400/0487 ,  C12M23/16 ,  G01N15/147 ,  G01N33/48 ,  G01N2015/0073 ,  G01N2015/1006

    摘要： The present disclosure relates to a new and improved microfluidic system which can precisely measure mechanical properties of a biological system such as single cell. The microfluidic system disclosed herein can enable measurement of cell mechanics at high throughputs, and can solve the channel clogging problem inherent in conventional bio-microfluidic devices. Further, the disclosed microfluidic system can eliminate cell-substrate interactions and provide more accurate estimate of mechanical properties of biological system. In an aspect, present disclosure provides a microfluidic system for determining mechanical properties of biological cells through single cell compression, wherein the system can include (a) a first microfluidic channel including an inlet and an outlet, the inlet being configured to receive a fluidic medium containing cells into the first microfluidic channel, and the outlet being configured to dispense the fluidic medium from the first microfluidic channel; and (b) a second microfluidic channel including a first air inlet and a second opposite air inlet; wherein the first and second microfluidic channels intersect perpendicularly to each other to form an intersection region; wherein each of the first and second air inlets receives pressurized air that flows towards the intersection region, and wherein the air flow creates, in the intersection region, an air/liquid interface which squeezes the cells as they pass through the air/liquid interface.

    MULTI-SHEATH FLOW AND ON-CHIP TERMINATING ELECTRODE FOR MICROFLUIDIC DIRECT-BLOTTING
    5.
    发明申请
    MULTI-SHEATH FLOW AND ON-CHIP TERMINATING ELECTRODE FOR MICROFLUIDIC DIRECT-BLOTTING 审中-公开
    微流控直接印刷用多层流动和片式端接电极

    公开(公告)号：WO2018031479A1

    公开(公告)日：2018-02-15

    申请号：PCT/US2017/045774

    申请日：2017-08-07

    申请人： LI-COR, INC.

    发明人： FURTAW, Michael D. ,  LAMB, Donald T. ,  MIDDENDORF, Lyle R.

    IPC分类号： G01N27/447 ,  G01N30/72 ,  B01L3/00

    CPC分类号： B01L3/502753 ,  B01L3/0268 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2300/06 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N1/405 ,  G01N27/44739 ,  G01N27/44756 ,  G01N27/44791

    摘要： Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.

    摘要翻译： 提供了使用通过出口通道连接到一个或多个鞘流通道的微流体分离柱来分离和分配材料的设备和方法。 分离的物质通过分离柱的流动至少部分地由分离柱内的第一电极与至少一个鞘流动通道内的终端电极之间的电压驱动。 分离柱，出口通道，鞘流通道和电极都在一个单片芯片内。 片上端接电极的存在允许将分离的材料夹带在鞘液中并喷射到可以不导电的表面上。 多鞘流的存在允许鞘液流体具有彼此不同的组成，同时减少鞘液流体进入分离柱的发生。

    SINGLE CELL WHOLE GENOME AMPLIFICATION VIA MICROPILLAR ARRAYS UNDER FLOW CONDITIONS
    6.
    发明申请
    SINGLE CELL WHOLE GENOME AMPLIFICATION VIA MICROPILLAR ARRAYS UNDER FLOW CONDITIONS 审中-公开
    通过微流体阵列在流动条件下进行的单细胞整体基因组扩增

    公开(公告)号：WO2017205304A1

    公开(公告)日：2017-11-30

    申请号：PCT/US2017/033885

    申请日：2017-05-22

    申请人： CORNELL UNIVERSITY

    发明人： CRAIGHEAD, Harold G. ,  TIAN, Harvey C.

    IPC分类号： C12M1/00 ,  C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/10 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/086 ,  C12Q1/6844 ,  C12Q2523/303 ,  C12Q2531/119 ,  C12Q2565/629

    摘要： The present invention relates to, inter alia, a microfluidic device for performing single cell genomic DNA isolation and amplification under flow. The microfluidic device comprises a solid substrate having one or more microfluidic channel system formed therein. Each microfluidic channel system of the microfluidic device comprises: (a) an intake region comprising a single microchannel; (b) a plurality of cell segregation microchannels; (c) a cell capture site located downstream of each cell segregation microchannel; and (d) a DNA capture array positioned downstream of the cell capture site and comprising a plurality of micropillars. Also disclosed is a whole genome amplification system that includes the microfluidic device of the present disclosure, as well as a method for conducting single cell DNA analysis via on-chip whole genome amplification while under flow, and a method for multiple displacement amplification (MDA) reactions of one or more nucleic acid sequence isolated single cells.

    摘要翻译： 本发明尤其涉及用于在流动下进行单细胞基因组DNA分离和扩增的微流体装置。 微流体装置包括具有在其中形成的一个或多个微流体通道系统的固体基底。 微流体装置的每个微流体通道系统包括：（a）包括单个微通道的进入区域; （b）多个细胞分离微通道; （c）位于每个细胞分离微通道下游的细胞捕获位点; 和（d）位于细胞捕获位点下游并包含多个微柱的DNA捕获阵列。 还公开了包括本公开的微流体装置的全基因组扩增系统，以及用于在流动下通过片上全基因组扩增进行单细胞DNA分析的方法，以及用于多位移扩增（MDA）的方法， 一个或多个核酸序列分离的单个细胞的反应。

    HIGH-THROUGHPUT PARTICLE CAPTURE AND ANALYSIS
    7.
    发明申请
    HIGH-THROUGHPUT PARTICLE CAPTURE AND ANALYSIS 审中-公开
    高通量粒子捕获和分析

    公开(公告)号：WO2017185098A1

    公开(公告)日：2017-10-26

    申请号：PCT/US2017/029202

    申请日：2017-04-24

    申请人： PURDUE RESEARCH FOUNDATION

    发明人： SAVRAN, Cagri Abdullah ,  GUR, Onur ,  CHANG, Chun-Li ,  HUANG, Wanfeng ,  JAIN, Rohil

    IPC分类号： B03C1/005 ,  B03C1/01 ,  B03C1/00

    CPC分类号： B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2300/0627 ,  B01L2300/0803 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26

    摘要： Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.

    摘要翻译： 描述了用于捕获与一个或多个磁珠结合的磁性靶标实体的微流体系统和方法。 该系统包括井阵列装置，其包括具有表面的基底，该表面具有在表面上以一个或多个阵列布置的多个井。 第一组孔邻近表面上的第一位置布置。 第二个和后续的阵列（如果存在）在第二个和随后的位置上依次排列在表面上。 当将液体样品添加到基底上并使其流动时，液体样品将首先流过第一阵列，然后按顺序流过第二和后续阵列。 第一个阵列中的孔每个都有一个大小，只允许一个目标实体进入孔中，而第一个阵列中的每个孔具有大致相同的大小。

    점탄성유체와 점성유체의 병행층류를 이용한 미소 입자 분리 및 세정 방법
    8.
    发明申请
    점탄성유체와 점성유체의 병행층류를 이용한 미소 입자 분리 및 세정 방법 审中-公开
    粘弹性流体与粘性流体并行层流的粒子分离与清洗方法

    公开(公告)号：WO2017126755A1

    公开(公告)日：2017-07-27

    申请号：PCT/KR2016/006991

    申请日：2016-06-30

    申请人： 한국과학기술원

    发明人： 성형진 ,  하병항

    IPC分类号： G01N35/08 ,  B01L3/00 ,  G01N35/00

    CPC分类号： B01D21/006 ,  B01L3/00 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2400/0487 ,  C07C31/225 ,  C08F26/10 ,  G01N15/0255 ,  G01N15/1056 ,  G01N33/491 ,  G01N35/08 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/1081 ,  G01N2015/1087

    摘要： 본 발명은 점탄성유체와 점성유체의 병행층류를 이용한 미소 입자 분리 및 세정 방법에 관한 것으로서, 점탄성유체 내에 포함된 원하는 목표 입자의 유효직경에 맞추어 유체칩 내에 형성된 유체 채널 내에 상기 점탄성유체와 함께 점성유체를 기설정된 유량비로 흘려 보내 상기 유체 채널 내에 형성된 이들의 병행층류 내에서 목표 입자에 대한 집속 위치의 변화를 통해 별도의 장치와 인력을 사용하지 않고도 좀더 쉽게 점탄성유체 내에 포함된 미소 입자들 중에서 원하는 목표 입자만을 점성유체쪽으로 분리해내거나 세정을 위해 점탄성유체 내에 포함된 목표 입자를 점성유체쪽으로 이동시킬 수 있도록 함으로써, 연구와 임상에서 쓰이는 천연 생체액들(Native Biofluids)은 대부분 점탄성유체이기 때문에 실험을 위해 세포/입자를 담을 용액을 바꾸거나 별도의 희석이 필요 없이 그대로 목표 입자의 분리 및 세정이 가능하고, 만일 점탄성유체인 천연 생체액들(Native Biofluids)의 완화시간(Relaxation time)이 부족하다면 이를 높여 줄 수 있는 인공 폴리머를 추가로 섞어주기만 하면 되기 때문에 시간당 처리량이 타 방법에 비해 매우 높고, 넓은 범위의 유량에서 고효율의 작업 능률을 보이기 때문에 정교한 펌핑 장치가 필요 없이 단순히 주사기를 밀어 펌핑하는 수작업 만으로도 고효율의 분리 및 세정 작업이 가능하도록 하는 효과를 갖는다.

    摘要翻译： 本发明涉及使用粘弹性流体和粘性流体的平行层流的微粒分离和清洁方法，其中在流体芯片中形成的流体通道 在其中，无需使用设备和人力的从目标粒子在形成于所述流体通道的粘性流体热交换器的层流的聚焦位置的变化平行分离更容易粘弹性流体的内部流过在与粘弹性流体以预定的流量 通过用于清洁naegeona仅向所期望的目标颗粒分离成从包含微粒的粘性流体，以朝向所述粘性流体移动包含在粘弹性流体中的目标粒子，在研究和临床（天然生物流体）中使用的天然生物流体被 由于它主要是粘弹性流体，因此建议使用含有细胞/ 盒装或弛豫时间（松弛时间）在单独的稀释是可能的分离和相同的目标粒子的洗涤而不需要，并且如果（母语生物流体）自然生物流体的粘弹性流体添加人造聚合物，其可以，如果不足增加它 如因为你只是混合允许需要复杂的泵送装置，而不简单地推动只是手动泵送效率注射器操作的分离和清洁，因为显示的在极高的流中的高效率的运行效率，和多种其他的方法中，可以通过比较 。

    CONSTRICTION-EXPANSION BLOOD PLASMA SEPARATION
    9.
    发明申请
    CONSTRICTION-EXPANSION BLOOD PLASMA SEPARATION 审中-公开
    限制 - 扩张血浆分离

    公开(公告)号：WO2017120317A1

    公开(公告)日：2017-07-13

    申请号：PCT/US2017/012308

    申请日：2017-01-05

    申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY

    发明人： SHATOVA, Tatyana, A. ,  JENSEN, Klavs, F. ,  ENGLE, Marissa, R.

    IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  G01N15/10 ,  G01N15/14 ,  G01N37/00

    CPC分类号： G01N33/491 ,  A61M1/3496 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2400/0478 ,  B01L2400/0487

    摘要： A portable, microfluidic blood plasma separation device is presented featuring a constriction-expansion design, which can produce up to about 100% purity for undiluted blood at least about 9% yield. This level of purity represents an improvement of at least one order of magnitude with increased yield compared to that achieved previously using passive separation. The system features high flow rates, 5-30 μΙ/min plasma collection, with minimal clogging and biofouling. The simple, portable blood plasma separation design can be hand-driven and can easily be incorporated with microfluidic or laboratory scale diagnostic assays. The separation system can be used in conjunction with portable analyte detection tests at concentrations well below clinical relevancy for undiluted whole blood.

    摘要翻译： 提出了一种便携式微流体血浆分离装置，其具有收缩 - 扩张设计，对于未稀释的血液可以产生高达约100％的纯度，至少约9％的收率。 与以前使用被动分离法相比，纯度水平至少提高了一个数量级，并且产量增加。 该系统具有高流速，5-30μl/ min血浆收集量，最小的堵塞和生物污染。 简单便携的血浆分离设计可以手动驱动，并且可以轻松与微流体或实验室规模的诊断分析结合使用。 分离系统可与便携式分析物检测试剂结合使用，浓度远低于未稀释全血的临床相关性。