公开(公告)号：WO2008120016A1

公开(公告)日：2008-10-09

申请号：PCT/GB2008/050228

申请日：2008-03-31

Applicant: UNIVERSITY OF SOUTHAMPTON ,  BROWN, Tom ,  KUMAR, Ravindra ,  EL-SAGHEER, Afaf H.

Inventor： BROWN, Tom ,  KUMAR, Ravindra ,  EL-SAGHEER, Afaf H.

IPC: C07H21/00 ,  B01J19/00

CPC classification number: C07H21/00

Abstract: Use of azide/alkyne coupling (click ligation) for oligonucleotide circularisation is proposed for both therapeutic and nanotechnology applications. Such non-templated single-strand circularisation may be the first step in building a more complex catenane by further templated-directed click ligation of olignucleotide sequences, e.g. a double- stranded cyclic oligonucleotide or pseudo-hexagon. Circularisation using click ligation may be used to improve stability of therapeutic oligonucleotides to enzyme degradation in vivo.

Abstract translation: 提出了用于寡核苷酸环化的叠氮化物/炔炔偶联（点击连接）用于治疗和纳米技术应用。 这种非模板化的单链环化可能是通过进一步模板化定向的双核苷酸序列的结合来构建更复杂的正链中的第一步。 双链环寡核苷酸或伪六边形。 使用点击连接的循环可以用于提高治疗性寡核苷酸在体内酶降解的稳定性。