公开(公告)号：WO2021124282A1

公开(公告)日：2021-06-24

申请号：PCT/IB2020/062232

申请日：2020-12-18

Applicant: FEI DEUTSCHLAND GMBH

Inventor： VOEGELE, Xaver ,  ACHTERMANN, Andreas ,  BOTTARO, Thierry ,  DAUM, Rainer

IPC: G02B21/33 ,  G02B27/00 ,  G02B21/00 ,  G02B21/0088 ,  G02B27/0006

Abstract: An imaging system configured for automatic application and/or removal of immersion media can include (i) a sample stage, (ii) an imaging assembly disposed on a first side of the sample stage and having an immersion objective configured to selectively align with an optical axis of the imaging system, and (iii) an applicator positioned to selectively interact with a lens surface of the immersion objective to deposit or remove immersion media.

公开(公告)号：WO2021104855A1

公开(公告)日：2021-06-03

申请号：PCT/EP2020/081657

申请日：2020-11-10

Applicant: THERMO FISHER SCIENTIFIC (BREMEN) GMBH ,  LIFE TECHNOLOGIES CORPORATION ,  FEI DEUTSCHLAND GMBH

Inventor： MAKAROV, Alexander ,  WARD, Michael ,  DAUM, Rainer

IPC: G01N21/64 ,  G01N21/71 ,  G01N35/00 ,  C12Q1/686 ,  C12Q1/6872 ,  H01J49/04 ,  H01J49/16

Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device (110) includes an optical assembly (112), a sample stage (114), and a receiver (116). The optical assembly (112) is disposed in an inverted position below the sample stage (114) and the receiver (116) is positioned above the sample stage (112). The optical assembly enables imaging of a sample disposed on the sample stage (114). The optical assembly (112) also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume (20) toward the receiver (116).

公开(公告)号：WO2022269442A1

公开(公告)日：2022-12-29

申请号：PCT/IB2022/055671

申请日：2022-06-18

Applicant: FEI DEUTSCHLAND GMBH

Inventor： DAUM, Rainer ,  VOEGELE, Xaver ,  PAHL, Gerrit

IPC: G02B21/02 ,  G02B7/02 ,  G02B7/08 ,  G02B21/245 ,  G02B21/248 ,  G02B27/005 ,  G02B7/023

Abstract: A system for selectively adjusting an objective lens correction collar includes a gear ring sized and shaped to fit about a correction collar of an objective lens. The system also includes an adjustment mechanism that has a motor operably connected to a complementary gear configured to selectively engage and cause movement of the gear ring, thereby adjusting the correction collar.

公开(公告)号：WO2022084861A1

公开(公告)日：2022-04-28

申请号：PCT/IB2021/059632

申请日：2021-10-19

Applicant: FEI DEUTSCHLAND GMBH

Inventor： DAUM, Rainer ,  PEDERSEN, Meike

IPC: G02B21/34 ,  B01L3/00 ,  C12M3/06 ,  G02B27/58

Abstract: A perfusion system for reducing or eliminating sample drift during microscopy imaging includes a sample chamber that has an inlet opening associated therewith. The perfusion system also includes a reservoir, which is in fluid communication with the inlet opening and positioned above the inlet opening in a direction opposite the force of gravity. The perfusion system includes an outlet opening associated with the sample chamber. Furthermore, the perfusion system includes a wick. A first portion of the wick forms a fluid-tight connection with the outlet opening. A second portion of the wick is disposed within a waste tank. A capillary tension of the wick contributes to a laminar flow of fluid across an optical detection area of the sample chamber.