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    ISOLATION OF PHOSPHOPROTEINS, GLYCOPROTEINS AND FRAGMENTS THEREOF
    1.
    发明申请
    ISOLATION OF PHOSPHOPROTEINS, GLYCOPROTEINS AND FRAGMENTS THEREOF 审中-公开
    磷酸二氢钙，糖蛋白及其片段的分离

    公开(公告)号：WO2012048331A3

    公开(公告)日：2012-06-21

    申请号：PCT/US2011055652

    申请日：2011-10-10

    Applicant: PHYNEXUS INC ,  POLATO FABIO ,  RAINER MATHIAS ,  GJERDE DOUGLAS T ,  BONN GUENTHER

    Inventor： POLATO FABIO ,  RAINER MATHIAS ,  GJERDE DOUGLAS T ,  BONN GUENTHER

    IPC: C12N9/00

    CPC classification number: C07K1/32

    Abstract: The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.

    Abstract translation: 本发明提供了选择性分离磷酸化和糖基化蛋白质及其片段的方法和装置。 金属阳离子用于沉淀含有磷酸基团和/或糖基团的蛋白质或蛋白质片段。 样品制备方法可用于几种类型的生物样品，包括HeLa细胞，食物和人脑脊液。 蛋白质被分离，回收并准备好通过质谱法或其他分析方法进行分析，允许检测限降低到飞甲的水平。 该方法和装置是蛋白质分析和诊断领域的宝贵工具。

    PURIFICATION OF NUCLEIC ACIDS
    2.
    发明申请
    PURIFICATION OF NUCLEIC ACIDS 审中-公开
    纯化核酸

    公开(公告)号：WO2012044991A1

    公开(公告)日：2012-04-05

    申请号：PCT/US2011/054322

    申请日：2011-09-30

    Applicant: PHYNEXUS, INC. ,  SUH, Chris ,  HOANG, Lee ,  GJERDE, Douglas, T.

    Inventor： SUH, Chris ,  HOANG, Lee ,  GJERDE, Douglas, T.

    IPC: B01L3/02

    CPC classification number: C12N15/1017

    Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

    Abstract translation: 本发明解决了在生长培养基存在下从细胞中分离核酸的问题。 本发明对于分离染色体外复制子如质粒特别有用。 细胞在其生长培养基的存在下裂解，并使用移液管尖端柱分离核酸。 液体处理机器人可用于同时分离多个样品中的核酸而不需要人为干预。

    BIOMOLECULE OPEN CHANNEL SOLID PHASE EXTRACTION SYSTEMS AND METHODS
    4.
    发明申请
    BIOMOLECULE OPEN CHANNEL SOLID PHASE EXTRACTION SYSTEMS AND METHODS 审中-公开

    公开(公告)号：WO2003104814A3

    公开(公告)日：2003-12-18

    申请号：PCT/US2003/014503

    申请日：2003-05-08

    Applicant: PHYNEXUS, INC. ,  GJERDE, Douglas, T. ,  HANNA, Christopher, P.

    Inventor： GJERDE, Douglas, T. ,  HANNA, Christopher, P.

    IPC: G01N30/00

    Abstract: An open capillary channel device for open tubular solid phase extraction of molecules capable of providing a tube enrichment factor of at least 1. The device comprises a channel (2, 12, 18, 42, 68, 98, 128, 200) having one end connected to a pump (44, 70,100, 218) for pumping liquid and gas, and the other end can be connected to an interface (26) for a protein chip sample applicator (32) or a mass spectrometer. The inner surface (4) of the channel, an extraction surface, can be bonded to an affinity binding agent such as a chelated metal, a protein, a sugar or nucleic acid. The method uses this device to bind analyte molecules from a sample solution to the affinity extraction surface and desorb analyte from the extraction surface with a desorbent liquid, with an extraction factor greater than 1.

    DEVICES AND METHODS FOR PURIFICATION, DETECTION AND USE OF BIOLOGICAL CELLS
    5.
    发明申请
    DEVICES AND METHODS FOR PURIFICATION, DETECTION AND USE OF BIOLOGICAL CELLS 审中-公开
    用于净化，检测和使用生物细胞的装置和方法

    公开(公告)号：WO2015154048A1

    公开(公告)日：2015-10-08

    申请号：PCT/US2015/024374

    申请日：2015-04-03

    Applicant: GJERDE, Douglas T.

    Inventor： GJERDE, Douglas T.

    IPC: B01D15/08

    CPC classification number: C07K1/16 ,  B01D15/166 ,  B01D15/3804 ,  B01D15/422 ,  B01D15/428 ,  C07K1/22

    Abstract: This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.

    Abstract translation: 本发明涉及用于纯化，检测和使用生物细胞的装置和方法。 可以使用柱（包括移液管尖端柱）从复杂的生物样品中纯化包括活的肿瘤，茎，免疫和精子细胞的多种细胞类型。 本发明的方法可以帮助研究，诊断和治疗癌症。 可以在柱上检测纯化的活细胞或从柱上洗脱并检测。 柱上的细胞可用作液相色谱的固定相。 可以除去，回收和分析细胞。

    DEVICES AND METHODS FOR PURIFICATION OF BIOLOGICAL CELLS
    6.
    发明申请
    DEVICES AND METHODS FOR PURIFICATION OF BIOLOGICAL CELLS 审中-公开
    用于净化生物细胞的装置和方法

    公开(公告)号：WO2014127314A1

    公开(公告)日：2014-08-21

    申请号：PCT/US2014/016637

    申请日：2014-02-15

    Applicant: PHYNEXUS, INC. ,  GJERDE, Douglas, T.

    Inventor： GJERDE, Douglas, T.

    IPC: C12N5/071 ,  C12N1/00 ,  C12M1/00

    CPC classification number: C12M47/04 ,  C12M47/02 ,  C12N1/02

    Abstract: A disclosure relating to devices and methods for purifying biological cells. For example, viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods disclosed can aid research, diagnosis and treatment of cancer.

    Abstract translation: 关于用于纯化生物细胞的装置和方法的公开内容。 例如，可以使用柱（包括移液管尖端柱）从复杂的生物样品中纯化可行的肿瘤，茎，免疫和精子细胞。 公开的方法可以帮助研究，诊断和治疗癌症。

    METHOD AND DEVICE FOR SAMPLE PREPARATION
    8.
    发明申请
    METHOD AND DEVICE FOR SAMPLE PREPARATION 审中-公开
    用于样品制备的方法和装置

    公开(公告)号：WO2005070141A2

    公开(公告)日：2005-08-04

    申请号：PCT/US2005000811

    申请日：2005-01-07

    Applicant: PHYNEXUS INC ,  GJERDE DOUGLAS T ,  HANNA CHRISTOPHER P

    Inventor： GJERDE DOUGLAS T ,  HANNA CHRISTOPHER P

    IPC: G01N30/00 ,  G01N30/60

    CPC classification number: G01N30/603 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J2220/54 ,  B01J2220/58 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  G01N30/02 ,  G01N2030/009 ,  G01N2035/1053 ,  B01D15/3804

    Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the invention provides columns having low bed volumes. In some embodiments, the invention provides columns characterized by low backpressure. In some embodiments, the invention provides methods characterized by the elution of analyte in a small volume of liquid.

    Abstract translation: 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，本发明提供具有低床体积的柱。 在一些实施方案中，本发明提供了以低背压为特征的色谱柱。 在一些实施方案中，本发明提供了以分析物在小体积液体中洗脱为特征的方法。

    OPEN CHANNEL SOLID PHASE EXTRACTION SYSTEMS AND METHODS
    9.
    发明申请
    OPEN CHANNEL SOLID PHASE EXTRACTION SYSTEMS AND METHODS 审中-公开
    开放通道固相萃取系统及方法

    公开(公告)号：WO2005015153A2

    公开(公告)日：2005-02-17

    申请号：PCT/US2004/014458

    申请日：2004-05-06

    Applicant: PHYNEXUS, INC. ,  GJERDE, Douglas, T. ,  HANNA, Christopher, P.

    Inventor： GJERDE, Douglas, T. ,  HANNA, Christopher, P.

    IPC: G01N

    CPC classification number: B82Y30/00 ,  G01N1/34 ,  G01N1/40 ,  G01N1/405 ,  G01N2030/009 ,  G01N2030/062

    Abstract: The invention provides, inter alia, methods, devices and reagents for the preparation and/or purification of analytes, including native and non-denatured biomolecules and multi-protein complexes, using solid-phase extraction channels. Some of the methods include steps where a solution is passed through the extraction channel at least twice. In some embodiments of the invention an analyte is eluted from the extraction channel in a small amount desorption solvent. Some of the methods include a step where liquid is blown out of the extraction channel prior to introducing another solution into the channel, e.g., a desorption solution.

    Abstract translation: 本发明尤其提供了使用固相提取通道来制备和/或纯化分析物的方法，装置和试剂，包括天然和非变性的生物分子和多蛋白复合物。 一些方法包括将溶液通过提取通道至少两次的步骤。 在本发明的一些实施方案中，分析物在少量解吸溶剂中从萃取通道中洗脱。 一些方法包括在将另一种溶液引入通道（例如解吸溶液）之前将液体吹出萃取通道的步骤。

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