IMPROVED METHODS FOR PRODUCING ISOBUTENE FROM 3-METHYLCROTONIC ACID

    公开(公告)号:WO2018206262A9

    公开(公告)日:2018-11-15

    申请号:PCT/EP2018/060051

    申请日:2018-04-19

    IPC分类号: C12P5/02 C12N1/21

    摘要: Described are methods for the production of isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl phosphate (DMAP) into a flavin-derived cofactor, wherein said method further comprises providing said DMAP enzymatically. Moreover, described are methods for the production of isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl pyrophosphate (DMAPP), wherein said method further comprises providing said DMAPP enzymatically.

    ENZYMATIC PRODUCTION OF ACRYLYL-COA OR ETHYLENE FROM GLYCEROL
    4.
    发明申请
    ENZYMATIC PRODUCTION OF ACRYLYL-COA OR ETHYLENE FROM GLYCEROL 审中-公开
    丙二醇或乙酸乙烯酯的生产

    公开(公告)号:WO2016097289A1

    公开(公告)日:2016-06-23

    申请号:PCT/EP2015/080482

    申请日:2015-12-18

    摘要: Described is a method for the production of acrylyl-CoA from glycerol comprising the following steps: (a) the enzymatic conversion of glycerol into 3-hydroxypropionaldehyde; (b) the enzymatic conversion of said 3-hydroxypropionaldehyde into 3-hydroxypropionyl-CoA; and (c) the enzymatic conversion of said 3-hydroxypropionyl-CoA into acrylyl-CoA. Further enzymatic conversion of acrylyl-CoA into other products are disclosed. Further described is a method for the production of ethylene comprising the enzymatic conversion of propionic acid into ethylene. It is described that the enzymatic conversion of propionic acid into ethylene can be achieved by making use of a cytochrome P450 fatty acid decarboxylase or a non-heme iron oxygenase. Further, it is described that said propionic acid can be obtained by the enzymatic conversion of acrylyl-CoA into said propionic acid.

    摘要翻译: 描述了由甘油制备丙烯酰辅酶A的方法,包括以下步骤:(a)将甘油酶促转化为3-羟基丙醛; (b)将所述3-羟基丙醛酶促转化成3-羟基丙酰辅酶A; 和(c)所述3-羟基丙酰辅酶A的酶促转化为丙烯酰辅酶A。 公开了将丙烯酰辅酶A进一步酶促转化成其它产物。 进一步描述了一种生产乙烯的方法,其包括将丙酸酶促转化成乙烯。 描述了丙酸进入乙烯的酶促转化可以通过使用细胞色素P450脂肪酸脱羧酶或非血红素铁加氧酶来实现。 此外,描述了所述丙酸可以通过将丙烯酰辅酶A酶促转化成所述丙酸而获得。

    PROCESS FOR THE ENZYMATIC PRODUCTION OF 1-BUTENE FROM 2-PENTENOYL-COA
    10.
    发明申请
    PROCESS FOR THE ENZYMATIC PRODUCTION OF 1-BUTENE FROM 2-PENTENOYL-COA 审中-公开
    2-PENTENOYL-COA生产1-丁烯的方法

    公开(公告)号:WO2016097293A1

    公开(公告)日:2016-06-23

    申请号:PCT/EP2015/080491

    申请日:2015-12-18

    摘要: Described is a method for the production of 2-pentenoic acid comprising the enzymatic conversion of 2-pentenoyl-CoA into 2-pentenoic acid comprising two enzymatic steps comprising (i) first enzymatically converting 2-pentenoyl-CoA into 2-pentenoyl phosphate; and (ii) then enzymatically converting the thus obtained 2-pentenoyl phosphate into said 2-pentenoic acid; or a single enzymatic reaction in which 2-pentenoyl-CoA is directly converted into 2-pentenoic acid by making use of a CoA transferase (EC 2.8.3.-), preferably a butyryl-CoA:acetate-CoA transferase (EC 2.8.3.8).

    摘要翻译: 描述了一种生产2-戊烯酸的方法,其包括将2-戊烯酰辅酶A酶促转化成2-戊烯酸,其包括两个酶步骤,包括(i)首先将2-戊烯酰辅酶A酶促转化为2-戊烯酰磷酸酯; 和(ii)然后将由此获得的2-戊烯酰磷酸酯酶促转化成所述2-戊烯酸; 或通过使用CoA转移酶(EC 2.8.3.-),优选丁酰辅酶A:乙酸酯-CoA转移酶(EC 2.8)直接将2-戊烯酰辅酶A直接转化为2-戊烯酸的单一酶促反应。 3.8)。