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公开(公告)号：WO2013037836A1
公开(公告)日：2013-03-21
申请号：PCT/EP2012/067856
申请日：2012-09-12
Inventor： GOBAA, Samy , LUTOLF, Matthias
CPC classification number: G01N33/5041 , B01J19/0046 , B01J2219/00385 , B01J2219/00509 , B01J2219/00596 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , C12N5/0068 , C12N2535/10 , C12Q1/02 , G01N33/5026 , G01N33/5032 , G03F7/20 , G03F7/32 , G03F7/36
Abstract: A method for preparing a topographically structured hydrogel microarray is described comprising the steps of a) providing one or more types of biomolecule(s) on top of micropillars of an array of micropillars, preferably by means of robotical spotting, b) providing a partially crosslinked hydrogel on a substrate, preferably attached to a substantially rigid and/or planar sub- strate, c) simultaneously soft-embossing a hydrogel microwell array and transferring the biomolecule(s) from the micropillars to the microwells by pressing the micropillars of the array of step a) onto the partially crosslinked layer of hydrogel of step b) until substantial completion of crosslinking and d) demolding the array of micropillars of step a) from the hydrogel microwell array of step c). The method according to the invention has the advantages of resulting in higher biochemical patterning precision, allowing for modulation of biochemical parameters by interfacing microarray manufacture with robotic technology and rendering the microarrays obtained compatible with existing read-out systems such as microscopes. Further, the elasticity of the hydrogel can be varied by tuning its shear modulus.
Abstract translation: 描述了一种用于制备地形结构的水凝胶微阵列的方法，其包括以下步骤：a）优选通过机器人点样，在微柱阵列的微柱体的顶部提供一种或多种类型的生物分子，b）提供部分交联的 基底上的水凝胶，优选地连接到基本上刚性和/或平面的基底上，c）同时软化压花水凝胶微孔阵列，并将生物分子从微透镜转移到微孔中，方法是通过将微阵列的微阵列 步骤a）到步骤b）的部分交联的水凝胶层上，直到基本完成交联，和d）将步骤a）的微柱阵列从步骤c）的水凝胶微孔阵列脱模。 根据本发明的方法具有产生更高的生物化学图案化精度的优点，允许通过将微阵列制造与机器人技术相连接并使获得的微阵列与现有的读出系统如显微镜相兼容来调制生物化学参数。 此外，水凝胶的弹性可以通过调节其剪切模量来改变。
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