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    OLIGOMERIC COMPOUNDS THAT FACILITATE RISC LOADING
    4.
    发明申请
    OLIGOMERIC COMPOUNDS THAT FACILITATE RISC LOADING 审中-公开
    易于加载的低分子化合物

    公开(公告)号：WO2005121370A3

    公开(公告)日：2006-05-26

    申请号：PCT/US2005019217

    申请日：2005-06-02

    申请人： ISIS PHARMACEUTICALS INC ,  SWAYZE ERIC E ,  BHAT BALKRISHEN ,  GRIFFEY RICHARD H ,  PRAKASH THAZHA P ,  ALLERSON CHARLES

    发明人： SWAYZE ERIC E ,  BHAT BALKRISHEN ,  GRIFFEY RICHARD H ,  PRAKASH THAZHA P ,  ALLERSON CHARLES

    IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/11 ,  C12Q1/68

    CPC分类号： C12N15/113 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/51 ,  C12N2310/3521

    摘要： The present invention provides double stranded compositions wherein one of the strands is useful in, for example, influencing the preferential loading the opposite strand into the RISC (or cleavage) complex. In particular, the present invention provides oligomeric compounds that comprise chemical modifications in at least one of the strands to drive loading of the opposite strand into the RISC (or cleavage) complex. Such modifications can be used to increase potency of duplex constructs that have been modified to enhance stability. Examples of chemical modifications that drive loading of the second strand include, but are not limited to, MOE (2'-O(CH 2 ) 2 OCH 3 ), 2'-O-methyl, -ethyl, -propyl, and -N-methylacetamide. Such modifications can be distributed throughout the strand, or placed at the 5' and/or 3' ends to make a gapmer motif on the sense strand. The activity of the 4'-thio gapmer RNA antisense strand can be improved by incorporating alternating MOE or MOE gapmer motif into the sense strand.

    摘要翻译： 本发明提供双链组合物，其中一条链可用于例如影响相对链优先加载到RISC（或切割）复合物中。 特别地，本发明提供了在至少一个链中包含化学修饰以驱动相反链加载到RISC（或切割）复合物中的低聚化合物。 可以使用这些修饰来增加经修饰以增强稳定性的双链构建体的效力。 驱动第二链的负载的化学修饰的实例包括但不限于MOE（2'-O（CH 2）2 OCH 3） ，2'-O-甲基， - 乙基， - 丙基和-N-甲基乙酰胺。 这样的修饰可以分布在整个链上，或者放置在5'和/或3'末端以在有义链上形成gapmer基序。 通过将交替的MOE或MOE gapmer基序并入有义链中，可以改善4'-硫代间隔RNA反义链的活性。

    OLIGOMERIC COMPOUNDS THAT FACILITATE RISC LOADING
    7.
    发明申请
    OLIGOMERIC COMPOUNDS THAT FACILITATE RISC LOADING 审中-公开
    易于加载的低分子化合物

    公开(公告)号：WO2005121370A2

    公开(公告)日：2005-12-22

    申请号：PCT/US2005/019217

    申请日：2005-06-02

    申请人： ISIS PHARMACEUTICALS, INC. ,  SWAYZE, Eric, E. ,  BHAT, Balkrishen ,  GRIFFEY, Richard, H. ,  PRAKASH, Thazha, P. ,  ALLERSON, Charles

    发明人： SWAYZE, Eric, E. ,  BHAT, Balkrishen ,  GRIFFEY, Richard, H. ,  PRAKASH, Thazha, P. ,  ALLERSON, Charles

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/113 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/51 ,  C12N2310/3521

    摘要： The present invention provides double stranded compositions wherein one of the strands is useful in, for example, influencing the preferential loading the opposite strand into the RISC (or cleavage) complex. In particular, the present invention provides oligomeric compounds that comprise chemical modifications in at least one of the strands to drive loading of the opposite strand into the RISC (or cleavage) complex. Such modifications can be used to increase potency of duplex constructs that have been modified to enhance stability. Examples of chemical modifications that drive loading of the second strand include, but are not limited to, MOE (2’-O(CH 2 ) 2 OCH 3 ), 2’-O-methyl, -ethyl, -propyl, and -N-methylacetamide. Such modifications can be distributed throughout the strand, or placed at the 5’ and/or 3’ ends to make a gapmer motif on the sense strand. The activity of the 4’-thio gapmer RNA antisense strand can be improved by incorporating alternating MOE or MOE gapmer motif into the sense strand.

    摘要翻译： 本发明提供双链组合物，其中一条链可用于例如影响相对链优先加载到RISC（或切割）复合物中。 特别地，本发明提供了在至少一个链中包含化学修饰以驱动相反链加载到RISC（或切割）复合物中的低聚化合物。 可以使用这些修饰来增加经修饰以增强稳定性的双链构建体的效力。 驱动第二链负载的化学修饰的实例包括但不限于MOE（2'-O（CH 2）2 OCH 3），2'-O-甲基， - 乙基， - 丙基和-N-甲基乙酰胺。 这样的修饰可以分布在整个链上，或者放置在5'和/或3'末端以在有义链上形成gapmer基序。 通过将交替的MOE或MOE gapmer基序并入有义链中，可以改善4'-硫代间隔RNA反义链的活性。

    METHODS OF RAPID DETECTION AND IDENTIFICATION OF BIOAGENTS USING microRNA
    10.
    发明申请
    METHODS OF RAPID DETECTION AND IDENTIFICATION OF BIOAGENTS USING microRNA 审中-公开
    使用微小RNA快速检测和识别生物量的方法

    公开(公告)号：WO2005033271A2

    公开(公告)日：2005-04-14

    申请号：PCT/US2004/028869

    申请日：2004-09-07

    申请人： ISIS PHARMACEUTICALS, INC. ,  GRIFFEY, Richard, H. ,  ECKER, David, J.

    发明人： GRIFFEY, Richard, H. ,  ECKER, David, J.

    IPC分类号： C12N

    CPC分类号： C12Q1/6888 ,  C12Q1/6816 ,  C12Q2600/156 ,  C12Q2531/113 ,  C12Q2525/15 ,  C12Q2565/627

    摘要： Methods for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of microRNA containing nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of microRNA containing nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a "base composition signature" (BCS) or molecular mass which is then matched against a database of base composition signatures or molecular masses, by which the species of the bioagent is identified.

    摘要翻译： 通过使用与含有衍生自生物制剂的含有核酸的微RNA的保守序列区杂交的引物与包含核酸扩增的微小RNA的组合来检测和识别未知生物标签，包括细菌，病毒等的方法， 唯一识别生物标签的序列区域。 结果是“基本组成特征”（BCS）或分子量，然后与基础组成特征或分子量的数据库进行匹配，通过该数据库识别出生物代谢物种。