公开(公告)号：WO2010075529A2

公开(公告)日：2010-07-01

申请号：PCT/US2009/069443

申请日：2009-12-23

Applicant: MASCOMA CORPORATION ,  HAU, Heidi ,  RICE, Charles ,  HERRING, Chris ,  MCBRIDE, John ,  SHAW, IV, Arthur J. ,  WISWALL, Erin

Inventor： HAU, Heidi ,  RICE, Charles ,  HERRING, Chris ,  MCBRIDE, John ,  SHAW, IV, Arthur J. ,  WISWALL, Erin

IPC: C12P7/06

CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention is directed to thermophilic gram-positive anaerobic host cells. The thermophilic gram-positive anaerobic host cells of the invention, for example Themoanaerobacterium sachharolyticum (" T. sacch "), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO 2 . In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T. sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Abstract translation: 本发明涉及嗜热革兰氏阳性厌氧宿主细胞。 本发明的嗜热革兰氏阳性厌氧宿主细胞，例如莎草精酵母（“茶糖酵母”）表达异源生物降解酶，例如纤维素酶，并且能够从纤维素产生有用的发酵产物。 有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。 为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。 此外，宿主细胞，例如，可以遗传改变宿主细胞，以进一步改善乙醇生产，例如通过破坏乙醇以外的有机产物的生产。

公开(公告)号：WO2010075529A9

公开(公告)日：2011-01-13

申请号：PCT/US2009069443

申请日：2009-12-23

Applicant: MASCOMA CORP ,  HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

Inventor： HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

IPC: C12N15/74 ,  C12P21/00

CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: Thermophilic gram-positive anaerobic host cells, for example Themoanaerobacterium sachharolyticum ("T sacch"), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Abstract translation: 嗜热革兰氏阳性厌氧宿主细胞，例如沙拉梭菌（“茶糖酵母”）表达异源生物降解酶，如纤维素酶，并能够从纤维素中产生有用的发酵产物。 有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。 为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。 此外，宿主细胞，例如T细胞宿主细胞，可进行遗传改变以进一步改善乙醇生产，例如通过破坏除乙醇以外的有机产物的生产。

公开(公告)号：WO2009035595A1

公开(公告)日：2009-03-19

申请号：PCT/US2008/010545

申请日：2008-09-10

Applicant: MASCOMA CORPORATION ,  CAIAZZA, Nicky ,  WARNER, Anne ,  HERRING, Chris

Inventor： CAIAZZA, Nicky ,  WARNER, Anne ,  HERRING, Chris

IPC: C07H21/00 ,  C12N15/74

CPC classification number: C12N15/70 ,  C12N15/74 ,  C12N15/81

Abstract: The present invention is directed to a replicative, thermostable plasmid. In particular, the present invention is directed to a replicative, thermostable plasmid comprising a sequence derived from the pB6A plasmid and at least one functional unit comprising a sequence that is not found in plasmid pB6A.

Abstract translation: 本发明涉及一种复制的热稳定性质粒。 特别地，本发明涉及包含源自pB6A质粒的序列和包含在质粒pB6A中未发现的序列的至少一个功能单元的复制热稳定质粒。

公开(公告)号：WO2010075529A3

公开(公告)日：2010-08-19

申请号：PCT/US2009069443

申请日：2009-12-23

Applicant: MASCOMA CORP ,  HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

Inventor： HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

IPC: C12N15/74 ,  C12P21/00

CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: Thermophilic gram-positive anaerobic host cells, for example Themoanaerobactenum sachharolyticum ("T sacch"), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2 In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide In addition, the host cell, for example, a T sacch. host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol

Abstract translation: 嗜热革兰氏阳性厌氧宿主细胞，例如莎草精酵母（“T sacch”）表达异源生物降解酶，例如纤维素酶，并且能够从纤维素生产有用的发酵产物有用的发酵产物包括例如乙醇，乙酸 酸，乳酸或CO 2为了提供最大的表达和活性水平，生物降解酶可以从密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与 信号肽另外，宿主细胞，例如T细胞。 宿主细胞，可进行遗传改造以进一步改善乙醇生产，例如通过破坏乙醇以外的有机产物的生产