公开(公告)号：WO2010075529A9

公开(公告)日：2011-01-13

申请号：PCT/US2009069443

申请日：2009-12-23

Applicant: MASCOMA CORP ,  HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

Inventor： HAU HEIDI ,  RICE CHARLES ,  HERRING CHRIS ,  MCBRIDE JOHN ,  SHAW IV ARTHUR J ,  WISWALL ERIN

IPC: C12N15/74 ,  C12P21/00

CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: Thermophilic gram-positive anaerobic host cells, for example Themoanaerobacterium sachharolyticum ("T sacch"), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Abstract translation: 嗜热革兰氏阳性厌氧宿主细胞，例如沙拉梭菌（“茶糖酵母”）表达异源生物降解酶，如纤维素酶，并能够从纤维素中产生有用的发酵产物。 有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。 为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。 此外，宿主细胞，例如T细胞宿主细胞，可进行遗传改变以进一步改善乙醇生产，例如通过破坏除乙醇以外的有机产物的生产。

公开(公告)号：WO2007130646A3

公开(公告)日：2008-11-20

申请号：PCT/US2007010958

申请日：2007-05-04

Applicant: UNIV ROCKEFELLER ,  RICE CHARLES ,  EVANS MATTHEW J ,  VON HAHN THOMAS

Inventor： RICE CHARLES ,  EVANS MATTHEW J ,  VON HAHN THOMAS

IPC: C07K16/00

CPC classification number: C07K16/28 ,  C07K16/2896 ,  C07K2317/76

Abstract: The invention relates to the discovery that the Claudin-1 protein functions as a co-receptor for entry of HCV into cells. Methods of inhibiting, preventing or mitigating HCV infections by inhibiting HCV interactions with Claudin-1 are provided. Methods of identifying agents or compounds that interfere with HCV interactions with Claudin-1 are also provided. Finally, useful kits, cell culture compositions, agents, and compounds related to the inhibition of HCV interactions with Claudin-1 are also disclosed.

Abstract translation: 本发明涉及发现紧密连接蛋白-1蛋白质作为HCV进入细胞的共同受体。 提供了通过抑制与Claudin-1的HCV相互作用来抑制，预防或减轻HCV感染的方法。 还提供了鉴定干扰HCV与Claudin-1相互作用的试剂或化合物的方法。 最后，还公开了与抑制与Claudin-1的HCV相互作用有关的有用的试剂盒，细胞培养组合物，试剂和化合物。

公开(公告)号：WO0189364A9

公开(公告)日：2003-07-10

申请号：PCT/US0116822

申请日：2001-05-23

Applicant: UNIV WASHINGTON ,  RICE CHARLES M III ,  BLIGHT KERIL J

Inventor： RICE CHARLES M III ,  BLIGHT KERIL J

IPC: C12N15/09 ,  A61K31/7088 ,  A61K39/00 ,  A61K39/29 ,  A61K48/00 ,  A61P1/16 ,  A61P31/14 ,  C07K14/18 ,  C12N5/10 ,  C12N5/22 ,  C12N15/85 ,  C12Q1/02 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

CPC classification number: C07H21/00 ,  A61K39/00 ,  A61K2039/53 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12N2770/24251 ,  C12N2840/203 ,  C12N2840/206 ,  C12Q1/707

Abstract: HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the provision of cells and host cells comprising the expression vectors. Methods for identifying a cell line that is permissive for infection with HCV are also provided, as are vaccines comprising the above polynucleotides in a pharmaceutically acceptable carrier. Additionally, methods for inducing immunoprotection to HCV in a primate are described, as are methods for testing a compound for inhibiting HCV replication.

Abstract translation: 描述了HCV变体。 变体包括包含非天然存在的HCV序列和HCV变体的多核苷酸，其具有转染效率和能够存活大于具有野生型多蛋白编码区的HCV的亚临床的能力。 还描述了包含上述多核苷酸和HCV变体的表达载体，以及提供包含表达载体的细胞和宿主细胞。 还提供了鉴定用于感染HCV的细胞系的方法，以及在药学上可接受的载体中包含上述多核苷酸的疫苗。 此外，还描述了在灵长类动物中诱导HCV的免疫保护的方法，以及用于测试抑制HCV复制的化合物的方法。

公开(公告)号：WO0189364A3

公开(公告)日：2003-01-23

申请号：PCT/US0116822

申请日：2001-05-23

Applicant: UNIV WASHINGTON ,  RICE CHARLES M III ,  BLIGHT KERIL J

Inventor： RICE CHARLES M III ,  BLIGHT KERIL J

IPC: C12N15/09 ,  A61K31/7088 ,  A61K39/00 ,  A61K39/29 ,  A61K48/00 ,  A61P1/16 ,  A61P31/14 ,  C07K14/18 ,  C12N5/10 ,  C12N5/22 ,  C12N15/85 ,  C12Q1/02 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

CPC classification number: C07H21/00 ,  A61K39/00 ,  A61K2039/53 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12N2770/24251 ,  C12N2840/203 ,  C12N2840/206 ,  C12Q1/707

Abstract: HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the provision of cells and host cells comprising the expression vectors. Methods for identifying a cell line that is permissive for infection with HCV are also provided, as are vaccines comprising the above polynucleotides in a pharmaceutically acceptable carrier. Additionally, methods for inducing immunoprotection to HCV in a primate are described, as are methods for testing a compound for inhibiting HCV replication.

Abstract translation: 描述了HCV变体。 所述变体包括含有非天然存在的HCV序列和HCV变体的多核苷酸，所述多核苷酸具有转染效率以及具有比野生型多蛋白编码区更高的HCV通过子通道的能力。 还描述了包含上述多核苷酸和HCV变体的表达载体，以及提供包含表达载体的细胞和宿主细胞。 还提供了用于鉴定容许HCV感染的细胞系的方法，以及在药学上可接受的载体中包含上述多核苷酸的疫苗。 另外，描述了用于在灵长类动物中诱导HCV免疫保护的方法，以及用于测试用于抑制HCV复制的化合物的方法。

公开(公告)号：WO2011153516A3

公开(公告)日：2012-03-29

申请号：PCT/US2011039192

申请日：2011-06-03

Applicant: MASCOMA CORP ,  BREVNOVA ELENA ,  MCBRIDE JOHN E ,  WISWALL ERIN ,  WENGER KEVIN S ,  CAIAZZA NICKY ,  HAU HEIDI H ,  ARGYROS AARON ,  AGBOGBO FRANK ,  RICE CHARLES F ,  BARRETT TRISHA ,  BARDSLEY JOHN S ,  FOSTER ABIGAIL S ,  WARNER ANNE K ,  MELLON MARK ,  SKINNER RYAN ,  SHIKHARE INDRANEEL ,  DEN HAAN RIAAN ,  GANDHI CHHAYAL V ,  BELCHER ALAN ,  RAJGARHIA VINEET B ,  FROEHLICH ALLAN C ,  DELEAULT KRISTEN M ,  STONEHOUSE EMILY ,  TRIPATHI SHITAL A ,  GOSSELIN JENNIFER ,  CHIU YIN-YING ,  XU HAOWEN ,  UNIV STELLENBOSCH

Inventor： BREVNOVA ELENA ,  MCBRIDE JOHN E ,  WISWALL ERIN ,  WENGER KEVIN S ,  CAIAZZA NICKY ,  HAU HEIDI H ,  ARGYROS AARON ,  AGBOGBO FRANK ,  RICE CHARLES F ,  BARRETT TRISHA ,  BARDSLEY JOHN S ,  FOSTER ABIGAIL S ,  WARNER ANNE K ,  MELLON MARK ,  SKINNER RYAN ,  SHIKHARE INDRANEEL ,  DEN HAAN RIAAN ,  GANDHI CHHAYAL V ,  BELCHER ALAN ,  RAJGARHIA VINEET B ,  FROEHLICH ALLAN C ,  DELEAULT KRISTEN M ,  STONEHOUSE EMILY ,  TRIPATHI SHITAL A ,  GOSSELIN JENNIFER ,  CHIU YIN-YING ,  XU HAOWEN

IPC: C12N1/15 ,  C12N1/19

CPC classification number: C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

Abstract: The present invention is directed to a. yeast strain, or strains, secreting a full suite, or any subset of that, full suite, of enzymes to hydrolyze com starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicelhiiose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.

Abstract translation: 本发明涉及a。 酵母菌株或菌株，分泌全套或全套酶的任何子集，以水解com淀粉，玉米纤维，木质纤维素（包括水解纤维素，半纤维素和木质素和碳水化合物之间的连接的酶）和 利用戊糖（木糖和阿拉伯糖）。 本发明还涉及在酵母中对于各种酶活性良好表达的蛋白质组。 所得菌株或菌株可以同时水解淀粉和纤维素。 所产生的菌株或菌株也可以代谢工程化以产生较少的甘油和吸收乙酸盐。 所得的菌株或菌株也可用于从颗粒淀粉生产乙醇而不液化。 所得到的菌株或菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前蔗糖分解酶负载下在SSF期间提高乙醇的产率。 此外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共同共同培养并用于从生物质原料生产乙醇。

公开(公告)号：WO2010075529A2

公开(公告)日：2010-07-01

申请号：PCT/US2009/069443

申请日：2009-12-23

Applicant: MASCOMA CORPORATION ,  HAU, Heidi ,  RICE, Charles ,  HERRING, Chris ,  MCBRIDE, John ,  SHAW, IV, Arthur J. ,  WISWALL, Erin

Inventor： HAU, Heidi ,  RICE, Charles ,  HERRING, Chris ,  MCBRIDE, John ,  SHAW, IV, Arthur J. ,  WISWALL, Erin

IPC: C12P7/06

CPC classification number: C12N15/74 ,  C12N9/2437 ,  C12P7/065 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention is directed to thermophilic gram-positive anaerobic host cells. The thermophilic gram-positive anaerobic host cells of the invention, for example Themoanaerobacterium sachharolyticum (" T. sacch "), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO 2 . In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T. sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

Abstract translation: 本发明涉及嗜热革兰氏阳性厌氧宿主细胞。 本发明的嗜热革兰氏阳性厌氧宿主细胞，例如莎草精酵母（“茶糖酵母”）表达异源生物降解酶，例如纤维素酶，并且能够从纤维素产生有用的发酵产物。 有用的发酵产物包括例如乙醇，乙酸，乳酸或二氧化碳。 为了提供最大的表达和活性水平，生物降解酶可以由密码子优化的核苷酸序列表达，可以在高效启动子的控制下表达，和/或可以与信号肽融合。 此外，宿主细胞，例如，可以遗传改变宿主细胞，以进一步改善乙醇生产，例如通过破坏乙醇以外的有机产物的生产。

公开(公告)号：WO2009080609A2

公开(公告)日：2009-07-02

申请号：PCT/EP2008/067609

申请日：2008-12-16

Applicant: BASF SE ,  FREUND, Annette ,  HADEN, Egon ,  MINORE, Jerry ,  VASSALOTTI, Paul M. ,  GARFIELD, Thomas ,  VANDECOEVERING, Jim ,  RICE, Charles ,  FELLOWS, Gary

Inventor： FREUND, Annette ,  HADEN, Egon ,  MINORE, Jerry ,  VASSALOTTI, Paul M. ,  GARFIELD, Thomas ,  VANDECOEVERING, Jim ,  RICE, Charles ,  FELLOWS, Gary

IPC: A01N37/50 ,  A01N47/24 ,  A23K1/00

CPC classification number: A23K1/1612 ,  A01N37/50 ,  A01N47/24 ,  A23K20/111 ,  A23K20/195 ,  A23K30/15 ,  A23K50/10 ,  A23K50/20 ,  A23K50/30

Abstract: The present invention relates to a method of increasing the milk and/or meat quantity of silage-fed animals comprising the steps: a) treating plants and/or propagules and/or sites where the plants are growing or are to grow with at least one strobilurin compound b) producing silage from the plants treated according to step a) c) feeding the milk and/or meat producing animals with the silage produced according to step b) made from the plants treated according to step a). Furthermore, the present invention relates to silage for feeding animals, produced from plants treated with at least one strobilurin compound prior to producing said silage. In addition, the present invention relates to the use of at least one strobilurin compound to increase the milk quantity of silage-fed milk-producing animals. Moreover, the present invention relates to the use of at least one strobilurin compound to increase the meat quantity of silage-fed meat-producing animals.

Abstract translation: 本发明涉及一种增加青饲料动物的牛奶和/或肉量的方法，包括以下步骤：a）处理植物和/或繁殖体和/或植物正在生长或将与至少一种 嗜球果伞菌素化合物b）从根据步骤a）处理的植物产生青贮饲料，c）向根据步骤a）处理的植物制备的根据步骤b）生产的青贮饲料喂养牛奶和/或肉生产动物。 此外，本发明涉及在生产所述青贮饲料之前，用至少一种嗜球果伞素化合物处理的植物产生的用于饲养动物的青贮饲料。 此外，本发明涉及使用至少一种嗜球果伞素化合物来增加饲喂青贮饲料的产奶动物的奶量。 此外，本发明涉及使用至少一种嗜球果伞素化合物来增加饲喂青饲料的产肉动物的肉量。

公开(公告)号：WO2009026380A1

公开(公告)日：2009-02-26

申请号：PCT/US2008/073737

申请日：2008-08-20

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF OKLAHOMA ,  RICE, Charles, V.

Inventor： RICE, Charles, V.

IPC: B05D3/00

CPC classification number: C09D133/14

Abstract: The present invention is thus directed to coating compositions and methods of their use, and to the objects coated therewith, for their use as implants and medical devices In particular, the medical devices and implants of the present invention comprise biomateπals which are coated with functionalized polymers having biomolecules conjugated thereto, or with the functionalized polymers to which such biomolecules can be conjugated to form the biofunctionalized polymer The biomolecules attached to the functionalized polymers contemplated herein may be defined as natural or synthetic chemical compounds which possess an ability to covalently bond to the functional group on the functionalized polymer or a spacer thereon, preferably via a sulfhydryl group thereon The functionalized or biofunctionalized polymers of the present invention may comprise spacer molecules which are covalently linked via sulfhydryl binds to the functional groups thereof for linking to the biomolecules of the biofunctionalized polymers.

Abstract translation: 因此，本发明涉及其用途的涂料组合物及其使用方法，以及涂覆于其上的物体，用作植入物和医疗装置。特别地，本发明的医疗装置和植入物包含涂覆有官能化聚合物的生物体 具有与其缀合的生物分子，或与可与其结合的这些生物分子的官能化聚合物形成生物官能化聚合物。本文涉及的官能化聚合物上连接的生物分子可以定义为具有共价键合到功能性的能力的天然或合成化学化合物 官能化聚合物或其上的间隔基，优选通过其上的巯基基团。本发明的官能化或生物官能化聚合物可以包含通过巯基共价连接的间隔基分子，其与其功能基团结合，用于连接生物器官的生物分子 聚合物。

公开(公告)号：WO2006098911A1

公开(公告)日：2006-09-21

申请号：PCT/US2006/007680

申请日：2006-03-03

Applicant: STATIC CONTROL COMPONENTS ,  JONES, James, H. ,  RICE, Charles Stanley, Jr. ,  HUCK, Donald, R. ,  HOSKINSON, Randall, William

Inventor： JONES, James, H. ,  RICE, Charles Stanley, Jr. ,  HUCK, Donald, R. ,  HOSKINSON, Randall, William

IPC: G03G15/08

CPC classification number: G03G15/0894 ,  G03G15/0882 ,  G03G2215/0687

Abstract: A seal assembly for use in an imaging cartridge includes a removable rigid insertion element having first and second sides. The seal assembly also includes a removable seal having a first side adhered to the first side of the removable rigid insertion element by a low tack adhesive, a second side coated with an adhesive layer, and a seal tail folded over a first end of the rigid insertion element and extending over at least a portion of the second side of the removable rigid insertion element. A release liner covers the adhesive layer and has a release liner tail.

Abstract translation: 用于成像盒的密封组件包括具有第一和第二侧面的可移除的刚性插入元件。 密封组件还包括可移除密封件，其具有通过低粘性粘合剂粘附到可移除的刚性插入元件的第一侧的第一侧，涂覆有粘合剂层的第二侧和在刚性的第一端上折叠的密封尾部 插入元件并且在可移除的刚性插入元件的第二侧的至少一部分上延伸。 剥离衬垫覆盖粘合剂层并且具有释放衬垫尾部。

公开(公告)号：WO2006096459A2

公开(公告)日：2006-09-14

申请号：PCT/US2006/007454

申请日：2006-03-03

Applicant: THE ROCKEFELLER UNIVERSITY ,  RICE, Charles ,  LINDENBACH, Brett, D. ,  EVANS, Matthew, J. ,  JONES, Christopher

Inventor： RICE, Charles ,  LINDENBACH, Brett, D. ,  EVANS, Matthew, J. ,  JONES, Christopher

IPC: C12Q1/70 ,  C07H21/04 ,  C12N7/00 ,  C12N15/86 ,  C12N5/06

CPC classification number: C12N7/00 ,  A61K2039/525 ,  C07K2319/61 ,  C12N15/86 ,  C12N2770/24243 ,  C12N2770/24251 ,  C12N2840/203 ,  C12Q1/18 ,  G01N33/5767 ,  G01N2333/18 ,  G01N2500/00

Abstract: The present invention provides infectious recombinant Hepatitis C Viruses (HCV), and vectors, cells and animals comprising the same. The present invention provides methods of producing infectious recombinant HCV, and their use in identifying anti-HCV therapeutic agents, as well as sequences of HCV associated with HCV pathogenesis.

Abstract translation: 本发明提供感染性重组丙型肝炎病毒（HCV），以及包含其的载体，细胞和动物。 本发明提供生产感染性重组HCV的方法及其在鉴定抗HCV治疗剂中的用途以及与HCV发病机制相关的HCV序列。