公开(公告)号：WO2010081114A2

公开(公告)日：2010-07-15

申请号：PCT/US2010020680

申请日：2010-01-11

Applicant: 20 20 GENESYSTEMS INC ,  JAMES WILLIAM M ,  RAIT VLADIMIR

Inventor： JAMES WILLIAM M ,  RAIT VLADIMIR

IPC: C12Q1/68

CPC classification number: G01N33/54306 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q2565/515 ,  C12Q2563/179

Abstract: A method of analyzing tissue sections in a manner that provides information about the presence and expression levels of multiple biomarkers at each location within the tissue section. The method comprises the preparation of membranes having covalently bound oligonucleotides and the use of those membranes for evaluation of various markers in the sample. The membranes may be arranged in stacks, wherein each layer has a different oligonucleotide capture strand. Transfer oligonucleotides complementary to the capture strands are attached through a cleavable bond to antibodies that recognize and bind to specific biomarkers present in the tissue sample. The tissue sample is exposed to the antibody-transfer strand conjugate and then treated with a cleaving reagent. Upon cleavage, the transfer strand migrates through the stack and binds to the capture strand. The level of expression of the biomarker may be determined by measuring expression of a reporter on the transfer strand.

Abstract translation: 以提供关于组织部分内的每个位置处的多个生物标志物的存在和表达水平的信息的方式分析组织切片的方法。 该方法包括制备具有共价结合寡核苷酸的膜，以及使用这些膜来评估样品中的各种标记物。 膜可以以堆叠方式布置，其中每层具有不同的寡核苷酸捕获链。 与捕获链互补的转移寡核苷酸通过可切割键连接到识别并结合存在于组织样品中的特异性生物标志物的抗体。 将组织样品暴露于抗体转移链缀合物，然后用切割试剂处理。 在切割时，转移链迁移通过堆叠并结合捕获链。 生物标志物的表达水平可以通过测量转移链上的报道分子的表达来确定。

公开(公告)号：WO2010081114A3

公开(公告)日：2010-07-15

申请号：PCT/US2010/020680

申请日：2010-01-11

Applicant: 20/20 GENESYSTEMS, INC. ,  JAMES, William, M. ,  RAIT, Vladimir

Inventor： JAMES, William, M. ,  RAIT, Vladimir

IPC: C12Q1/68

Abstract: A method of analyzing tissue sections in a manner that provides information about the presence and expression levels of multiple biomarkers at each location within the tissue section. The method comprises the preparation of membranes having covalently bound oligonucleotides and the use of those membranes for evaluation of various markers in the sample. The membranes may be arranged in stacks, wherein each layer has a different oligonucleotide capture strand. Transfer oligonucleotides complementary to the capture strands are attached through a cleavable bond to antibodies that recognize and bind to specific biomarkers present in the tissue sample. The tissue sample is exposed to the antibody-transfer strand conjugate and then treated with a cleaving reagent. Upon cleavage, the transfer strand migrates through the stack and binds to the capture strand. The level of expression of the biomarker may be determined by measuring expression of a reporter on the transfer strand.