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    METHOD AND SYSTEM FOR NUCLEIC ACID SEQUENCING
    3.
    发明申请
    METHOD AND SYSTEM FOR NUCLEIC ACID SEQUENCING 审中-公开

    公开(公告)号：WO2018219581A1

    公开(公告)日：2018-12-06

    申请号：PCT/EP2018/061288

    申请日：2018-05-03

    Applicant: SIEMENS HEALTHCARE GMBH

    Inventor： HEUCKMANN, Johannes ,  MARIOTTI, Erika

    IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6869

    CPC classification number: C12Q1/6869 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/165 ,  C12Q2539/101 ,  C12Q2563/179

    Abstract: The present invention relates to methods and systems for nucleic acid sequencing. In particular, the present invention relates to methods and systems for reducing the number of false-positives in nucleic acid sequencing. The method comprises: aligning a plurality of genetic reads to a reference genetic sequence; grouping the genetic reads into a plurality of groups; creating a consensus sequence for each group of the plurality of groups by setting a representation of the most abundant nucleotide man_p or a tag N based on a ratio r; and identifying a variation as a true variation if a ratio r* between the number of consensus sequences comprising the tag N at a specific position p and the number of the consensus sequences comprising the variation at the specific position p is below a threshold t*.

    METHODS AND COMPOSITIONS RELATED TO BARCODE ASSISTED ANCESTRAL SPECIFIC EXPRESSION (BAASE)
    5.
    发明申请
    METHODS AND COMPOSITIONS RELATED TO BARCODE ASSISTED ANCESTRAL SPECIFIC EXPRESSION (BAASE) 审中-公开
    与条形码辅助的起始特异性表达（BAASE）相关的方法和组合物

    公开(公告)号：WO2018031864A1

    公开(公告)日：2018-02-15

    申请号：PCT/US2017/046454

    申请日：2017-08-11

    Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

    Inventor： BROCK, Amy ,  AL'KHAFAJI, Aziz

    IPC: C12N15/10 ,  C12N15/86 ,  C12N15/90 ,  C12N15/113 ,  C04B40/06

    CPC classification number: C12N15/1065 ,  C12N9/22 ,  C12N15/10 ,  C12N15/102 ,  C12N15/11 ,  C12N15/111 ,  C12N15/63 ,  C12N2310/20 ,  C12N2740/16043 ,  C12Q2563/179

    Abstract: Disclosed herein are methods and platforms related to modulating expression of a gene of interest within a select population of cells comprising: providing a population of cells; providing a vehicle, plasmid, vector or recombinant virus, or equivalent thereof, capable of stably expressing a guide nucleic acid comprising randomized barcodes, thereby producing a population of barcoded cells; allowing said barcoded cell to divide, thereby forming a barcoded progeny of cells; saving an aliquot of cells; identifying the barcode in a lineage of interest from the barcoded progeny of cells; reconstituting the aliquot of saved cells, and transforming the reconstituted aliquot of cells with a transcriptional element comprising a transcriptional effector, the barcode of the lineage of interest, and a gene of interest; utilizing the transcriptional effector to modify expression of the gene of interest within the lineage of interest.

    Abstract translation: 本文公开了与在选择的细胞群体内调节感兴趣基因的表达有关的方法和平台，其包括：提供细胞群体; 提供能够稳定表达包含随机化条形码的指导核酸的载体，质粒，载体或重组病毒或其等同物，从而产生条形码细胞群; 允许所述条形码化细胞分裂，从而形成条形码化的细胞后代; 节省等分的细胞; 从条形码化细胞后代鉴定感兴趣谱系中的条形码; 重构保存的细胞的等分试样，并用包含转录效应物，感兴趣谱系的条形码和感兴趣的基因的转录元件转化重构的细胞等分试样; 利用转录效应子修饰感兴趣的谱系内感兴趣的基因的表达。

    METHODS AND COMPOSITIONS TO IDENTIFY, QUANTIFY, AND CHARACTERIZE TARGET ANALYTES AND BINDING MOIETIES
    8.
    发明申请
    METHODS AND COMPOSITIONS TO IDENTIFY, QUANTIFY, AND CHARACTERIZE TARGET ANALYTES AND BINDING MOIETIES 审中-公开
    确定，量化和表征目标分析和结合年龄的方法和组合物

    公开(公告)号：WO2017127556A1

    公开(公告)日：2017-07-27

    申请号：PCT/US2017/014151

    申请日：2017-01-19

    Applicant: CDI LABORATORIES, INC.

    Inventor： ZHU, Heng ,  QIAN, Jiang ,  PINO, Ignacio

    IPC: C12Q1/68 ,  G01N33/50 ,  C40B30/04

    CPC classification number: C12Q1/68 ,  C12Q1/6837 ,  C12Q1/6874 ,  G01N33/54306 ,  G01N2458/10 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/514

    Abstract: Proximity coupling and sequencing methods to screen identify, validate and/or characterize interactions between analytes and binding moieties are disclosed. Also disclosed herein are proximity coupling methods and sequencing methods to determine or quantify levels of target analytes. The disclosed methods can be multiplexed in two dimensions, and can be used to determine the affinity and specificity of each of a plurality of binding moieties for each of a plurality of target analytes. Also disclosed herein are substrates, arrays, and reagents for use in the methods, and methods of their preparation.

    Abstract translation: 公开了用于筛选鉴定，验证和/或表征分析物和结合部分之间的相互作用的接近偶联和测序方法。 本文还公开了确定或定量目标分析物水平的接近偶联方法和测序方法。 所公开的方法可以以二维方式多路复用，并且可以用于确定多个靶分析物中的每一个的多个结合部分中的每一个的亲和力和特异性。 本文还公开了用于所述方法及其制备方法的底物，阵列和试剂。

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