公开(公告)号：WO1996041154A1

公开(公告)日：1996-12-19

申请号：PCT/US1996010007

申请日：1996-06-07

Applicant: NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY ,  WEETALL, Howard, Hayyam ,  HELMERSON, Kristian, Peter ,  KISHORE, Roni, Bakhru

Inventor： NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY

IPC: G01N21/39

CPC classification number: H05H3/04 ,  G01N33/54313 ,  G01N33/54373

Abstract: Tightly focused beams of laser light are used as "optical tweezers" to trap and manipulate polarizable objects such as microspheres of glass or latex with diameters on the order of 4.5 mu m. When analytes are allowed to adhere to the microspheres, small quantities of these analytes can be manipulated, thus allowing their detection and quantitation even when amounts and concentrations of the analytes are extremely small. Illustrative examples include measuring the strength needed to break antibody-antigen bonds and the detection of DNA sequences.

Abstract translation: 紧密聚焦的激光束被用作“光学镊子”以捕获和操纵可极化物体，例如直径为4.5μm的玻璃或胶乳微球。 当允许分析物粘附到微球时，可以操纵少量的这些分析物，从而即使当分析物的量和浓度非常小时也能够进行检测和定量。 示例性实例包括测量断裂抗体 - 抗原键所需的强度和DNA序列的检测。