公开(公告)号：WO0218585A8

公开(公告)日：2003-04-24

申请号：PCT/US0141888

申请日：2001-08-28

Applicant: WYETH CORP ,  KOVACS GERALD R ,  KOWALSKI JACEK ,  GANGOLLI SEEMA S ,  VASILAKIS NIKOS ,  ZAMB TIMOTHY

Inventor： KOVACS GERALD R ,  KOWALSKI JACEK ,  GANGOLLI SEEMA S ,  VASILAKIS NIKOS ,  ZAMB TIMOTHY

IPC: C12N15/09 ,  A61K35/76 ,  A61K38/17 ,  A61K39/12 ,  A61K48/00 ,  A61P25/28 ,  A61P31/00 ,  A61P35/00 ,  A61P37/08 ,  C07H21/04 ,  C07K14/08 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N9/12 ,  C12N15/86 ,  C12N15/863 ,  C12Q1/70 ,  A61K39/00

CPC classification number: C12N15/86 ,  A61K38/1709 ,  A61K48/00 ,  A61K2039/5256 ,  C07H21/04 ,  C12N2710/24143 ,  C12N2710/24144 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2830/002

Abstract: The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting recombinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions and pharmaceutical formulations are provided that comprise replicon particles of the invention. Methods for generating an immune response or producing a pharmaceutical effect are also provided.

Abstract translation: 本发明一般涉及重组多核苷酸，正链RNA病毒（psRNAV）重组表达载体和包装系统。 包装系统基于通过合并包含重组多核苷酸的重组痘病毒载体来表达辅助功能。 公开了使用这些包装系统获得psRNAV复制子颗粒的方法。 提供了包含本发明的复制子颗粒的免疫原性组合物和药物制剂。 还提供了产生免疫应答或产生药物作用的方法。

公开(公告)号：WO2002018585A3

公开(公告)日：2002-03-07

申请号：PCT/US2001/041888

申请日：2001-08-28

Applicant: AMERICAN CYANAMID COMPANY ,  KOVACS, Gerald, R. ,  VASILAKIS, Nikos ,  KOWALSKI, Jacek ,  ZAMB, Timothy

Inventor： KOVACS, Gerald, R. ,  VASILAKIS, Nikos ,  KOWALSKI, Jacek ,  ZAMB, Timothy

IPC: C12N15/86

Abstract: The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting recombinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions and pharmaceutical formulations are provided that comprise replicon particles of the invention. Methods for generating an immune response or producing a pharmaceutical effect are also provided.

公开(公告)号：WO0200883A3

公开(公告)日：2003-02-06

申请号：PCT/US0120157

申请日：2001-06-22

Applicant: AMERICAN CYANAMID CO ,  PARKS CHRISTOPHER L ,  SIDHU MOHINDERJIT S ,  WALPITA PRAMILA ,  KOVACS GERALD R ,  UDEM STEPHEN A

Inventor： PARKS CHRISTOPHER L ,  SIDHU MOHINDERJIT S ,  WALPITA PRAMILA ,  KOVACS GERALD R ,  UDEM STEPHEN A

IPC: C12N15/09 ,  A61K35/76 ,  A61K39/175 ,  A61P31/12 ,  A61P31/14 ,  C07K14/12 ,  C07K14/13 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N7/02 ,  C12N15/40 ,  C12N15/45 ,  C12N15/86

CPC classification number: C12N7/00 ,  A61K2039/5256 ,  C07K14/005 ,  C12N2760/18422 ,  C12N2760/18451

Abstract: This invention relates to a method for recombinantly producing via rescue canine distemper virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the canine distemper virus as an attenuated and/or infectious viruses. The recombinant viruses can be prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and rearrangements, in the genome can be obtained.

Abstract translation: 本发明涉及通过救援犬瘟热病毒，非分段，负义的单链RNA病毒和由其形成的免疫原性组合重组生产的方法。 另外的实施方案涉及生产犬瘟热病毒作为减毒和/或感染性病毒的方法。 可以从cDNA克隆制备重组病毒，因此，可以获得在基因组中具有确定的变化（包括核苷酸/多核苷酸缺失，插入，取代和重排）的病毒。

公开(公告)号：WO0218585A2

公开(公告)日：2002-03-07

申请号：PCT/US0141888

申请日：2001-08-28

Applicant: AMERICAN CYANAMID CO ,  KOVACS GERALD R ,  VASILAKIS NIKOS ,  KOWALSKI JACEK ,  ZAMB TIMOTHY ,  GANGOLLI SEEMA S

Inventor： KOVACS GERALD R ,  VASILAKIS NIKOS ,  KOWALSKI JACEK ,  ZAMB TIMOTHY ,  GANGOLLI SEEMA S

IPC: C12N15/09 ,  A61K35/76 ,  A61K38/17 ,  A61K39/12 ,  A61K48/00 ,  A61P25/28 ,  A61P31/00 ,  A61P35/00 ,  A61P37/08 ,  C07H21/04 ,  C07K14/08 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N9/12 ,  C12N15/86 ,  C12N15/863 ,  C12Q1/70 ,  C12N15/00

CPC classification number: C12N15/86 ,  A61K38/1709 ,  A61K48/00 ,  A61K2039/5256 ,  C07H21/04 ,  C12N2710/24143 ,  C12N2710/24144 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2830/002

Abstract: The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting recombinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions and pharmaceutical formulations are provided that comprise replicon particles of the invention. Methods for generating an immune response or producing a pharmaceutical effect are also provided.

Abstract translation: 本发明一般涉及重组多核苷酸，正链RNA病毒（psRNAV）重组表达载体和包装系统。 包装系统基于通过共同重组包含重组多核苷酸的重组痘病毒载体的辅助功能的表达。 公开了使用这些包装系统获得psRNAV复制子颗粒的方法。 提供了包含本发明的复制子颗粒的免疫原性组合物和药物制剂。 还提供了产生免疫应答或产生药效的方法。