公开(公告)号：WO2020028729A1

公开(公告)日：2020-02-06

申请号：PCT/US2019/044763

申请日：2019-08-01

Applicant: MAMMOTH BIOSCIENCES, INC. ,  CHEN, Janice Sha ,  TEHRANCHI, Ashley ,  LANE, Andrew Besancon ,  BROUGHTON, James Paul ,  HARRINGTON, Lucas Benjamin ,  TSALOGLOU, Maria-Nefeli ,  MIAO, Xin ,  FASCHING, Clare Louise ,  SINGH, Jasmeet ,  GALARZA, Pedro Patrick Draper

Inventor： CHEN, Janice Sha ,  TEHRANCHI, Ashley ,  LANE, Andrew Besancon ,  BROUGHTON, James Paul ,  HARRINGTON, Lucas Benjamin ,  TSALOGLOU, Maria-Nefeli ,  MIAO, Xin ,  FASCHING, Clare Louise ,  SINGH, Jasmeet ,  GALARZA, Pedro Patrick Draper

IPC: C12Q1/6897 ,  C12Q1/683 ,  C12Q1/6818

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.