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公开(公告)号：WO2023028444A1
公开(公告)日：2023-03-02
申请号：PCT/US2022/075228
申请日：2022-08-19
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： HARRINGTON, Lucas Benjamin , PAEZ-ESPINO, David , RAUCH, Benjamin Julius , TYMOSHENKO, Stepan
IPC: C12N9/22 , C12N15/113 , C12Q1/682 , C12Q1/6827
Abstract: Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.
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公开(公告)号：WO2022241059A2
公开(公告)日：2022-11-17
申请号：PCT/US2022/028865
申请日：2022-05-11
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： BROUGHTON, James Paul , CHEN, Janice Sha , CHING, Jesus , FASCHING, Clare Louise , HARRINGTON, Lucas Benjamin , KREINDLER, Lior , MCKAY, Bridget Ann Paine , PAEZ-ESPINO, David , RAUCH, Benjamin Julius , VEROSLOFF, Matthew , WRIGHT, William Douglass
IPC: C12N9/22 , C12N15/11 , C12N15/113 , C12Q1/6876 , C12N2310/20 , C12Q1/6816
Abstract: The present disclosure provides compositions of effector proteins. The compositions may comprise engineered guide nucleic acids. Also disclosed are the methods and systems for detecting and modifying target nucleic acids using the same. The compositions may provide nucleic acid modification and/or detection at relatively high temperatures.
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公开(公告)号：WO2022132833A1
公开(公告)日：2022-06-23
申请号：PCT/US2021/063405
申请日：2021-12-14
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： CHEN, Janice Sha , HENDRIKS, Carley Gelenter , FASCHING, Clare Louise , MIAO, Xin , ALPAY, Nazmiye Emel , BROUGHTON, James Paul , HAWKINS, Elizabeth M. , VEROSLOFF, Matthew S. , HUBBELL, Sophia
IPC: C12Q1/6813 , C12Q1/6876 , C12N15/113 , C12N9/22
Abstract: The present disclosure provides single-buffer systems that enable efficient and rapid amplification and programmable nuclease enzyme mediated reactions. The single-buffer systems and reagent compositions therein may be used for assaying for a nucleic acid sequence from a sample.
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4.发明申请
公开(公告)号：WO2020142754A3
公开(公告)日：2020-07-09
申请号：PCT/US2020/012276
申请日：2020-01-03
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： HARRINGTON, Lucas Benjamin , CHEN, Janice Sha , BROUGHTON, James Paul , GALARZA, Pedro Patrick Draper , HARTONO, Wiputra J. , WITTE, Isaac Paterson , SINGH, Jasmeet
IPC: C12N9/22 , C12Q1/6816 , C12Q1/6897
Abstract: Disclosed herein are compositions, kits, and methods related to improved Cas activity. Through compositions and kits disclosed herein and practice of methods disclosed herein, one attains improved Cas activity such as Cas12 activity relative to Cas proteins in the art such as LbCas12. Further described herein are methods to detect target nucleic acid using a programmable nuclease system. Often, the target nucleic acids are present in at low frequency in the sample. Provided herein are methods for enriching these target nucleic acids for detection. Also described herein are methods to insert a PAM sequence into a target sequence of interest for use in a detection comprising a programmable nuclease.
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公开(公告)号：WO2022241032A1
公开(公告)日：2022-11-17
申请号：PCT/US2022/028834
申请日：2022-05-11
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： WRIGHT, William Douglass , RAUCH, Benjamin Julius , DELOUGHERY, Aaron , BROUGHTON, James Paul
IPC: C12N15/86
Abstract: The present disclosure describes CRISPR/Cas proteins and guide RNA combinations that are capable of modifying nucleic acids at high temperatures. These compositions are especially useful for systems and methods of nucleic acid detection, including diagnostic devices.
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Patent Agency Ranking
公开(公告)号：WO2022140572A1
公开(公告)日：2022-06-30
申请号：PCT/US2021/064904
申请日：2021-12-22
Applicant: MAMMOTH BIOSCIENCES, INC.
IPC: A61P35/00 , C12N9/22 , C12N15/11 , C12N15/113
Abstract: Disclosed herein, in certain embodiments, are methods of inducing cell cycle arrest, apoptosis, cell death, or a combination thereof, in a cell or population of cells. Also disclosed herein are methods of treating a disease or condition in an individual in need thereof comprising inducing cell cycle arrest, apoptosis, cell death, or a combination thereof in a population of cells in the individual. The cell or population of cells may comprise a nucleic acid sequence associated with a disease or condition, including an autoimmune disease, cancer, or an infectious disease. The methods described herein generally comprise contacting cells with a CRISPR-associated protein and a guide nucleic acid.
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公开(公告)号：WO2022006536A1
公开(公告)日：2022-01-06
申请号：PCT/US2021/040338
申请日：2021-07-02
Inventor： CHIU, Charles , DENG, Xian, Ding , CHEN, Janice, Sha , BROUGHTON, James , VEROSLOFF, Matthew
IPC: C12Q1/6888 , C12N15/11 , C12Q1/6844 , C12Q1/70
Abstract: The disclosure provides for CRTSPR-Cas-based detection of SARS-CoV-2 using recombinase polymerase amplification, and uses thereof.
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公开(公告)号：WO2021243308A2
公开(公告)日：2021-12-02
申请号：PCT/US2021/035031
申请日：2021-05-28
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： CHING, Jesus , BROUGHTON, James Paul , TSALOGLOU, Maria-Nefeli , LEE, Phillip You Fai , PATNO, Timothy James , CHEN, Janice Sha , SHAPIRO, Sarah Jane , CORDOVA, James Raymond , EASTMAN, Brian J. , LOWMILLER, Donald Allan , FASCHING, Clare Louise , HENDRIKS, Carley Gelenter
IPC: A01H3/00 , B01L3/00 , C12N15/11 , C12N15/74 , C12Q1/6809 , B01L2300/0636 , B01L2300/0681 , B01L2300/0816 , B01L2300/0825 , B01L2300/0864 , B01L2400/0487 , B01L2400/0644 , B01L2400/065 , B01L2400/0655 , B01L3/5027 , B01L3/502738 , B01L7/52 , C12N2310/20 , C12Q1/6806 , C12Q1/701 , C12Q2600/156 , G01N27/00
Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.
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公开(公告)号：WO2021216772A1
公开(公告)日：2021-10-28
申请号：PCT/US2021/028481
申请日：2021-04-21
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： RAUCH, Benjamin , WRIGHT, William Douglass , HARTONO, Wiputra , HARRINGTON, Lucas Benjamin , RHINES, Clarissa Oriel , CHEN, Janice Sha , BROUGHTON, James Paul , DELOUGHERY, Aaron
IPC: C12N9/22 , C12N15/113
Abstract: The present disclosure provides compositions and methods of use for Type V CRISPR/Cas proteins. Type V CRISPR/Cas nucleases may be configured to bind nucleic acids in a sequence specific manner. Such a binding event may activate the Type V CRISPR/Cas nuclease for sequence non-specific trans-collateral cleavage of single-stranded nucleic acids. Provided herein are methods leveraging Type V CRISPR/Cas nuclease trans-collateral cleavage for identifying nucleic acid sequences.
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公开(公告)号：WO2023092136A1
公开(公告)日：2023-05-25
申请号：PCT/US2022/080278
申请日：2022-11-21
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： DELOUGHERY, Aaron , RAUCH, Benjamin , RHINES, Clarissa Oriel , YUNANDA, Fnu , TYMOSHENKO, Stepan
Abstract: Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.
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