公开(公告)号：WO2012058714A1

公开(公告)日：2012-05-10

申请号：PCT/AU2011/001400

申请日：2011-10-31

Applicant: TPI ENTERPRISES LTD. ,  COOMBS, Justin, Taylor ,  RITCHIE, Jarrod, David ,  TESTER, Mark, Alfred ,  LIGHTFOOT, Damien ,  JHA, Deepa

Inventor： COOMBS, Justin, Taylor ,  RITCHIE, Jarrod, David ,  TESTER, Mark, Alfred ,  LIGHTFOOT, Damien ,  JHA, Deepa

IPC: A01H5/08 ,  C07D221/28 ,  C12N15/53 ,  A01H5/10 ,  C07D489/02

CPC classification number: C12N9/0006 ,  C07D489/02 ,  C12N9/0004 ,  C12N9/0071 ,  C12N15/8243 ,  C12P17/18 ,  C12Y101/01247 ,  C12Y114/11031 ,  C12Y114/11032

Abstract: The present invention relates to genetically modified plants of the species Papaver bracteatum wherein the type or amount of one or more alkaloids produced by the plants has been modified. Specifically, the genetically modified plants have an increased expression of one or more of thebaine 6-O-demethylase, codeine O- demethylase and/or codeinone reductase relative to wild type P. bracteatum such that the genetically modified poppy plants produce an increased quantity of an alkaloid selected from codeine, oripavine and/or morphine relative to a wild type P. bracteatum. Also provided are progeny plants having the genetically modified poppy plants described above as a parent; mutant or derivative plants of the aforementioned plants; reproductive material derived from, straw produced from, straw concentrate produced from, latex derived from, or one or more isolated cells derived from, the aforementioned plants. Methods for producing an alkaloid from the aforementioned plants are also provided, together with nucleic acid and amino acid sequence variants of the 6-O-demethylase and codeine O-demethylase genes.

Abstract translation: 本发明涉及种植罂粟种的遗传修饰植物，其中由植物产生的一种或多种生物碱的类型或数量已被修饰。 具体而言，遗传修饰的植物相对于野生型P.actactactum具有增加的一种或多种蒂巴因6-O-脱甲基酶，可待因O-脱甲基酶和/或依尼可酮还原酶的表达，使得转基因罂粟植物产生增加量的 相对于野生型P.bracteatum，选自可待因，oripavine和/或吗啡的生物碱。 还提供了具有上述转基因罂粟植物作为亲本的后代植物; 上述植物的突变体或衍生植物; 衍生自生殖材料的生殖材料，由生产的秸秆浓缩物产生的秸秆，衍生自上述植物的胶原衍生自或一种或多种分离的细胞。 还提供了从上述植物生产生物碱的方法以及6-O-脱甲基酶和可待因O-去甲基化酶基因的核酸和氨基酸序列变体。

公开(公告)号：WO2013163668A1

公开(公告)日：2013-11-07

申请号：PCT/AU2012/000457

申请日：2012-04-30

Applicant: TPI ENTERPRISES LTD. ,  COOMBS, Justin, Taylor ,  RITCHIE, Jarrod, David ,  TESTER, Mark, Alfred ,  LIGHTFOOT, Damien ,  JHA, Deepa

Inventor： COOMBS, Justin, Taylor ,  RITCHIE, Jarrod, David ,  TESTER, Mark, Alfred ,  LIGHTFOOT, Damien ,  JHA, Deepa

IPC: C12N15/53 ,  A01H5/10 ,  C07D489/02 ,  A01H5/08 ,  C07D221/28

CPC classification number: C12N15/8243 ,  C07D489/02 ,  C12N9/0006 ,  C12N9/0071 ,  C12Y101/01247 ,  C12Y114/11031 ,  C12Y114/11032

Abstract: The present invention relates to genetically modified plants of the species Papaver bracteatum wherein the type or amount of one or more alkaloids produced by the plants has been modified. Specifically, the genetically modified plants have an increased expression of one or more of thebaine 6-O-demethylase, codeine O- demethylase and/or codeinone reductase relative to wild type P. bracteatum such that the genetically modified poppy plants produce an increased quantity of an alkaloid selected from codeine, oripavine and/or morphine relative to a wild type P. bracteatum. Also provided are progeny plants having the genetically modified poppy plants described above as a parent; mutant or derivative plants of the aforementioned plants; reproductive material derived from, straw produced from, straw concentrate produced from, latex derived from, or one or more isolated cells derived from, the aforementioned plants. Methods for producing an alkaloid from the aforementioned plants are also provided, together with nucleic acid and amino acid sequence variants of the 6-O-demethylase and codeine O-demethylase genes.

Abstract translation: 本发明涉及种植罂粟种的遗传修饰植物，其中由植物产生的一种或多种生物碱的类型或数量已被修饰。 具体而言，遗传修饰的植物相对于野生型P.actactactum具有增加的一种或多种蒂巴因6-O-脱甲基酶，可待因O-脱甲基酶和/或依尼可酮还原酶的表达，使得转基因罂粟植物产生增加量的 相对于野生型P.bracteatum，选自可待因，oripavine和/或吗啡的生物碱。 还提供了具有上述转基因罂粟植物作为亲本的后代植物; 上述植物的突变体或衍生植物; 衍生自生殖材料的生殖材料，由生产的秸秆浓缩物产生的秸秆，衍生自上述植物的胶原衍生自或一种或多种分离的细胞。 还提供了从上述植物生产生物碱的方法以及6-O-脱甲基酶和可待因O-去甲基化酶基因的核酸和氨基酸序列变体。