公开(公告)号：WO0105942A3

公开(公告)日：2001-05-25

申请号：PCT/IB0001076

申请日：2000-07-20

Applicant: EPITECH S A ,  HUNZIKER THOMAS ,  LIMAT ALAIN

Inventor： HUNZIKER THOMAS ,  LIMAT ALAIN

IPC: C12N11/02 ,  A61K35/36 ,  A61L27/00 ,  A61P17/02 ,  C12N5/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/06

CPC classification number: A61L27/60 ,  A61L27/3804 ,  A61L2430/18 ,  C12N5/0627 ,  C12N5/0628 ,  C12N5/063 ,  C12N2510/04 ,  C12N2533/30

Abstract: The present invention relates to the treatment of skin defects by organotypically-cultured autologous keratinocytes isolated from the outer root sheath of anagen or growing hair. Methods for primary, as well as subsequent organotypic cultures (i.e., epidermal equivalents) in fully-defined media supplemented by autologous human serum and substances isolated form blood components, with minimal allogeneic biological supplements, are disclosed herein. Techniques to prepare epidermal equivalents for transplantation by use of a biocompatible glue are also disclosed herein.

Abstract translation: 本发明涉及通过从生长发育或生长的毛发的外根鞘分离的器官培养的自体角化细胞来治疗皮肤缺陷。 在本文中公开了用自体人血清补充的完全限定培养基中的初级和随后的器官型培养物（即表皮等效物）和分离自血液成分的物质与最小同种异体生物补充剂的方法。 本文还公开了通过使用生物相容性胶水来制备用于移植的表皮等同物的技术。