公开(公告)号：WO2004083819A3

公开(公告)日：2005-08-11

申请号：PCT/US2004008332

申请日：2004-03-17

Applicant: TRACE GENETICS INC ,  ESHLEMAN JASON ,  MALHI RIPAN

Inventor： ESHLEMAN JASON ,  MALHI RIPAN

IPC: G01N20060101 ,  G01N33/48 ,  G01N37/00

CPC classification number: C12Q1/68 ,  G06F19/28 ,  C12Q2563/185 ,  C12Q2563/179

Abstract: A molecular identification marker for biological samples taken, for example, at a crime scene to confirm the chain of custody and identity of the biological samples at the time of analysis in a laboratory and method of use. The molecular identification marker also acts as an indicator of cross-contamination between marked samples or between reference and unknown biological samples. One embodiment of the molecular identification marker is a segment of DNA with at least one mutagenic primer site configured to produce a unique variant after mutagenic PCR, a segment configured to amplify for a selected forensic locus; and a segment configured for use with a probe to quantify the molecular identification marker.

Abstract translation: 用于生物样品的分子识别标记，例如在犯罪现场，以在实验室和使用方法分析时确认生物样品的保管链和身份。 分子识别标记还可用作标记样品之间或参考和未知生物样品之间交叉污染的指标。 分子识别标记的一个实施方案是具有至少一个诱变引物位点的DNA片段，其配置为在致突变PCR之后产生唯一变体，构建成扩增选定法证位点的片段; 以及被配置为与探针一起使用以对量化分子识别标记的片段。

公开(公告)号：WO2004083819A2

公开(公告)日：2004-09-30

申请号：PCT/US2004/008332

申请日：2004-03-17

Applicant: TRACE GENETICS, INC ,  ESHLEMAN, Jason ,  MALHI, Ripan

Inventor： ESHLEMAN, Jason ,  MALHI, Ripan

IPC: G01N

CPC classification number: C12Q1/68 ,  G06F19/28 ,  C12Q2563/185 ,  C12Q2563/179

Abstract: A molecular identification marker for biological samples taken, for example, at a crime scene to confirm the chain of custody and identity of the biological samples at the time of analysis in a laboratory and method of use. The molecular identification marker also acts as an indicator of cross-contamination between marked samples or between reference and unknown biological samples. One embodiment of the molecular identification marker is a segment of DNA with at least one mutagenic primer site configured to produce a unique variant after mutagenic PCR, a segment configured to amplify for a selected forensic locus; and a segment configured for use with a probe to quantify the molecular identification marker.

Abstract translation: 用于生物样品的分子识别标记，例如在犯罪现场拍摄，以在实验室和使用方法分析时确认生物样品的保管链和身份。 分子识别标记还可用作标记样品之间或参考和未知生物样品之间交叉污染的指标。 分子识别标记的一个实施方案是具有至少一个诱变引物位点的DNA片段，其配置为在致突变PCR之后产生唯一变体，构建成扩增选定法证位点的片段; 以及被配置为与探针一起使用以对量化分子识别标记的片段。