公开(公告)号：WO2014188269A1

公开(公告)日：2014-11-27

申请号：PCT/IB2014/001008

申请日：2014-05-23

Applicant: MARKLUND, Stefan, L. ,  ANDERSEN, Peter, M. ,  BRÄNNSTRÖM, Thomas ,  OLIVEBERG, Mikael

Inventor： MARKLUND, Stefan, L. ,  ANDERSEN, Peter, M. ,  BRÄNNSTRÖM, Thomas ,  OLIVEBERG, Mikael

IPC: G01N33/68 ,  A61K38/44 ,  A61K39/395 ,  C07K16/40 ,  C12N9/02

CPC classification number: C07K16/40 ,  A61K38/446 ,  C07K2317/20 ,  C07K2317/23 ,  C07K2317/30 ,  C12N9/0089 ,  G01N33/6896 ,  G01N2333/90283 ,  G01N2800/10

Abstract: The present invention provides a method for typing an aggregate (including a fibril) of a polypeptide in a test sample, such as human superoxide dismutase (hSODI), wherein the polypeptide is capable of forming at least two structurally distinct types of aggregates, said method comprising (i) determining the reactivity of the aggregate in the test sample with one or more different antibody preparations in a panel of antibody preparations, wherein each antibody preparation in the panel has binding specificity to a peptide sequence derived from the amino acid sequence of the polypeptide, such that different antibody preparations in the panel have binding specificity to different peptides, and wherein at least one of the antibody preparations of the panel, for which reactivity to the aggregate is determined in step (i), displays differential reactivity to at least two structurally distinct types of the aggregate; and (ii) attributing a type to the aggregate in the test sample, based on the determined level(s) of reactivity with the one or more antibody preparations in the panel as determined in step (i).

Abstract translation: 本发明提供了一种在测试样品例如人超氧化物歧化酶（hSODI）中分型多肽的聚集体（包括原纤维）的方法，其中所述多肽能够形成至少两种结构不同类型的聚集体，所述方法 其包括（i）测定样品中聚集体与一​​组抗体制剂中的一种或多种不同抗体制剂的反应性，其中该组中的每种抗体制剂对衍生自该抗体制剂的氨基酸序列的肽序列具有结合特异性 多肽，使得该组中的不同抗体制剂对不同的肽具有结合特异性，并且其中在步骤（i）中测定与聚集体的反应性的面板抗体制剂中的至少一种抗体制剂至少显示出至少 两种结构不同类型的骨料; 以及（ii）基于在步骤（i）中确定的与所述面板中的一种或多种抗体制剂的反应性的确定水平来确定测试样品中的总体类型。

公开(公告)号：WO1996014060A1

公开(公告)日：1996-05-17

申请号：PCT/IB1995000979

申请日：1995-11-03

Applicant: MARKLUND, Stefan, L. ,  STRÅLIN, Pontus

IPC: A61K31/00

CPC classification number: A61K31/00

Abstract: The present invention relates to the use of a substance for the manufacture of a composition for stimulating the release of EC-SOD from cells or stimulating the synthesis of EC-SOD in cells. In particular, the invention relates to the use of a substance for the manufacture of a composition for prophylaxis or treatment of a disease or disorder connected with the presence or formation of superoxide radicals and other toxic intermediates derived from the superoxide radical. Further, the invention relates to a method for determining the effect of a substance with respect to stimulating the release of EC-SOD from cells or stimulating the synthesis of EC-SOD in cells and to substances which have been selected by said method. Within the scope of the invention is a method of preventing, diminishing, controlling or inhibiting a disease or disorder connected with the presence or formation of superoxide radicals and other toxic intermediates derived from the superoxide radical in a patient who has been established to have a high risk of developing a such disease or disorder, or who has developed a such disease or disorder, the method comprising administering an effective amount of a substance which is capable of stimulating the release of EC-SOD from cells or stimulating the synthesis of EC-SOD in cells.

Abstract translation: 本发明涉及用于制造用于刺激细胞中EC-SOD释放或刺激细胞中EC-SOD合成的组合物的物质的用途。 特别地，本发明涉及用于制备用于预防或治疗与由超氧自由基衍生的超氧化物基团和其它有毒中间体的存在或形成相关的疾病或病症的组合物的用途。 此外，本发明涉及一种用于确定物质在刺激细胞中EC-SOD释放或刺激细胞中EC-SOD的合成以及通过所述方法选择的物质方面的作用的方法。 在本发明的范围内是一种预防，减少，控制或抑制与已经建立为具有高的氧化应激的患者中超氧自由基和超氧化物基团的其它有毒中间体的存在或形成有关的疾病或病症的方法 发生这种疾病或病症的风险，或已经发展了这种疾病或病症的方法，该方法包括给予有效量的能够刺激EC-SOD从细胞释放或刺激EC-SOD合成的物质 在细胞中。