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    SEQUENCING CHICKEN ANTIBODY REPERTOIRES FOLLOWING HYPERIMMUNIZATION AND THE IDENTIFICATION OF ANTIGEN-SPECIFIC MONOCLONAL ANTIBODIES
    1.
    发明申请
    SEQUENCING CHICKEN ANTIBODY REPERTOIRES FOLLOWING HYPERIMMUNIZATION AND THE IDENTIFICATION OF ANTIGEN-SPECIFIC MONOCLONAL ANTIBODIES 审中-公开

    公开(公告)号：WO2017100289A2

    公开(公告)日：2017-06-15

    申请号：PCT/US2016/065331

    申请日：2016-12-07

    Applicant: CAMAS INCORPORATED

    Inventor： FAGERLUND, Jeff ,  MITTENESS, Bradley, M. ,  PHILLIPS, Connie

    IPC: A61K38/02

    CPC classification number: C07K16/00 ,  C07K2317/23 ,  C07K2317/622

    Abstract: Neutralizing antibodies through recombinant protein expression were identified by mining antibody repertoires by high-throughput second generation sequencing. Sequencing across the majority of light and heavy chain variable regions of chicken immunoglobulins was performed at two immunological time points: a non-immune (naive) state and a post-hyperimmunization state. The mRNA was extracted from unsorted and unselected PBMC populations and identification of antigen-specific antibody sequences leveraged the significant disparity of abundance of an individual B cell clone in non-immune and immunized states. Through bioinformatic analysis, candidate amino acid sequences for variable heavy chain and light chains were identified. Recombinant polypeptides with the binding domains having the identified amino acid sequences for variable heavy chain and light chains were expressed and screened using immunoassays to confirm antigen-specificity.

    VACCINE DEVELOPMENT AGAINST THE POULTRY RED MITE DERMANYSSUS GALLINAE
    4.
    发明申请
    VACCINE DEVELOPMENT AGAINST THE POULTRY RED MITE DERMANYSSUS GALLINAE 审中-公开
    疫苗的发展对家禽红M DER螨的影响

    公开(公告)号：WO2016062832A2

    公开(公告)日：2016-04-28

    申请号：PCT/EP2015074542

    申请日：2015-10-22

    Applicant: FRAUNHOFER GES ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E V

    Inventor： ULBERT SEBASTIAN ,  RODRIGUES MAKERT DOS SANTOS GUSTAVO

    IPC: A61K39/00

    CPC classification number: C07K16/18 ,  A61K39/0003 ,  A61K2039/552 ,  A61K2039/55566 ,  C07K14/43531 ,  C07K2317/11 ,  C07K2317/23

    Abstract: The Poultry Red Mite (PRM) Dermanyssus gallinae causes high economic losses and is among the most devastating parasites in poultry farming world-wide. Different chemical, physical and biological strategies try to control the expansion of PRM. However, a solution to this problem still has highest priority. We present here an innovative method for the development of an immunological control strategy, based on the identification of mite protein antigens which elicit antibodies with anti-mite activity in the immunized chicken. Hens were immunized with different PRM protein extracts and IgY-antibodies were extracted from the eggs. A PRM in vitro feeding assay which used chicken blood spiked with these IgY enabled the detection of antibodies which caused PRM mortality. A novel combination of 2D-gel analysis and comparative analysis of antibody binding patterns followed by proteomic approaches led to the identification of candidate antigens responsible for the generation of such protective antibodies. These results show the high potential of this strategy for the development of a vaccine against the poultry mite Dermanyssus gallinae.

    Abstract translation: 家禽红螨（PRM）Dermanyssus gallinae引起高度经济损失，并且是世界范围内家禽养殖中最具破坏性的寄生虫。 不同的化学，物理和生物学策略试图控制PRM的扩展。 但是，解决这个问题仍然是最重要的。 我们在这里介绍了一种创新的免疫控制策略的开发方法，该方法基于鉴定在被免疫的鸡中引发具有抗螨活性的抗体的螨蛋白抗原。 用不同的PRM蛋白提取物免疫母鸡，并从卵中提取IgY抗体。 PRM体外进食测定使用掺入了这些IgY的鸡血来检测导致PRM死亡的抗体。 2D-凝胶分析和抗体结合模式的比较分析的新颖组合随后是蛋白质组学方法，导致鉴定负责产生此类保护性抗体的候选抗原。 这些结果表明这种策略在开发针对家禽螨Dermanyssus gallinae的疫苗方面具有很高的潜力。

    AGGREGATES OF SUPEROXIDE DISMUTASE
    5.
    发明申请
    AGGREGATES OF SUPEROXIDE DISMUTASE 审中-公开
    超氧化物歧化酶的聚集

    公开(公告)号：WO2014188269A1

    公开(公告)日：2014-11-27

    申请号：PCT/IB2014/001008

    申请日：2014-05-23

    Applicant: MARKLUND, Stefan, L. ,  ANDERSEN, Peter, M. ,  BRÄNNSTRÖM, Thomas ,  OLIVEBERG, Mikael

    Inventor： MARKLUND, Stefan, L. ,  ANDERSEN, Peter, M. ,  BRÄNNSTRÖM, Thomas ,  OLIVEBERG, Mikael

    IPC: G01N33/68 ,  A61K38/44 ,  A61K39/395 ,  C07K16/40 ,  C12N9/02

    CPC classification number: C07K16/40 ,  A61K38/446 ,  C07K2317/20 ,  C07K2317/23 ,  C07K2317/30 ,  C12N9/0089 ,  G01N33/6896 ,  G01N2333/90283 ,  G01N2800/10

    Abstract: The present invention provides a method for typing an aggregate (including a fibril) of a polypeptide in a test sample, such as human superoxide dismutase (hSODI), wherein the polypeptide is capable of forming at least two structurally distinct types of aggregates, said method comprising (i) determining the reactivity of the aggregate in the test sample with one or more different antibody preparations in a panel of antibody preparations, wherein each antibody preparation in the panel has binding specificity to a peptide sequence derived from the amino acid sequence of the polypeptide, such that different antibody preparations in the panel have binding specificity to different peptides, and wherein at least one of the antibody preparations of the panel, for which reactivity to the aggregate is determined in step (i), displays differential reactivity to at least two structurally distinct types of the aggregate; and (ii) attributing a type to the aggregate in the test sample, based on the determined level(s) of reactivity with the one or more antibody preparations in the panel as determined in step (i).

    Abstract translation: 本发明提供了一种在测试样品例如人超氧化物歧化酶（hSODI）中分型多肽的聚集体（包括原纤维）的方法，其中所述多肽能够形成至少两种结构不同类型的聚集体，所述方法 其包括（i）测定样品中聚集体与一​​组抗体制剂中的一种或多种不同抗体制剂的反应性，其中该组中的每种抗体制剂对衍生自该抗体制剂的氨基酸序列的肽序列具有结合特异性 多肽，使得该组中的不同抗体制剂对不同的肽具有结合特异性，并且其中在步骤（i）中测定与聚集体的反应性的面板抗体制剂中的至少一种抗体制剂至少显示出至少 两种结构不同类型的骨料; 以及（ii）基于在步骤（i）中确定的与所述面板中的一种或多种抗体制剂的反应性的确定水平来确定测试样品中的总体类型。

    A METHOD OF DETECTING SURROGATE MARKERS FOR ACTIVE TUBERCULOSIS IN A SERUM SAMPLE
    9.
    发明申请
    A METHOD OF DETECTING SURROGATE MARKERS FOR ACTIVE TUBERCULOSIS IN A SERUM SAMPLE 审中-公开
    检测血清样本中主动管腔的血浆标记物的方法

    公开(公告)号：WO2012007903A3

    公开(公告)日：2012-03-22

    申请号：PCT/IB2011053108

    申请日：2011-07-12

    Applicant: UNIV PRETORIA ,  VERSCHOOR JAN ADRIANUS ,  BEUKES MERVYN

    Inventor： VERSCHOOR JAN ADRIANUS ,  BEUKES MERVYN

    IPC: G01N33/543 ,  G01N33/569

    CPC classification number: G01N33/53 ,  C07K16/44 ,  C07K2317/23 ,  C07K2317/33 ,  C07K2317/622 ,  G01N33/558 ,  G01N33/5695

    Abstract: The invention provides a method of detecting surrogate markers for active tuberculosis in a serum sample. The surrogate markers are selected from serum mycolic acid antigen, serum anti-mycolic acid antibodies or both. The method includes the steps of combining the serum sample with a labelled monoclonal immunoglobulin antibody or fragment thereof to mycolic acids to produce a combined serum sample, the antibody or fragment thereof not substantially cross-reacting with cholesterol and the label being selected so that binding of the labelled antibody to immobilized mycolic acid antigen of mycobacterial origin produces a detectable signal and combining a blank sample with the labelled monoclonal immunoglobulin antibody or fragment thereof to mycolic acid to produce a combined blank sample. The method includes exposing both samples to immobilised mycolic acid antigen of mycobacterial origin or a synthetic analogue or analogues thereof so that the labelled immunoglobulin antibodies or fragments thereof in each sample bind to the immobilised antigen to produce detectable signals. If the surrogate markers are present, the signal produced by the blank sample will be stronger than that produced by the serum sample because of inhibition of binding of the labelled antibody in the serum sample arising from prior binding of the labelled antibody with the mycolic acid antigen in the serum sample or by competitive binding of serum anti- mycolic acid antibodies in the serum sample to the immobilised mycolic acid antigen or both.

    Abstract translation: 本发明提供一种检测血清样品中活性结核病替代标记物的方法。 替代标记选自血清霉酚酸抗原，血清抗霉菌酸抗体或两者。 该方法包括以下步骤：将血清样品与标记的单克隆免疫球蛋白抗体或其片段组合成霉菌酸以产生组合的血清样品，所述抗体或其片段与胆固醇基本上不交叉反应，并且所述标签被选择为使得 分枝杆菌来源的固定的霉菌酸抗原的标记抗体产生可检测信号，并将空白样品与标记的单克隆免疫球蛋白抗体或其片段组合成霉菌酸以产生组合的空白样品。 该方法包括将两个样品暴露于分枝​​杆菌来源的固定的霉菌酸抗原或其合成的类似物或类似物，使得每个样品中的标记的免疫球蛋白抗体或其片段与固定的抗原结合以产生可检测的信号。 如果存在替代标记，则空白样品产生的信号将比由血清样品产生的信号更强，因为抑制标记的抗体在标记抗体与霉菌酸抗原的先前结合之间产生的血清样品中的结合 在血清样品中或通过血清样本中血清抗霉菌酸抗体与固定的霉菌酸抗原或两者的竞争性结合。

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