公开(公告)号：WO2007120302A3

公开(公告)日：2007-12-06

申请号：PCT/US2006061414

申请日：2006-11-30

Applicant: WISCONSIN ALUMNI RES FOUND ,  ABBOTT NICHOLAS L ,  DE PABLO JUAN J ,  PALECEK SEAN P ,  LOCKWOOD NATHAN A ,  MOHR JEFFREY C ,  MURPHY CHRISTOPHER J ,  HUANG ELISE

Inventor： ABBOTT NICHOLAS L ,  DE PABLO JUAN J ,  PALECEK SEAN P ,  LOCKWOOD NATHAN A ,  MOHR JEFFREY C ,  MURPHY CHRISTOPHER J ,  HUANG ELISE

IPC: C12N5/00 ,  C12M3/00 ,  C12N5/0735

CPC classification number: C12N5/0068 ,  C12N5/0606 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract translation: 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供用于成像活细胞对细胞外基质组分的改变（例如，重组）的方法和装置。

公开(公告)号：WO2007149926A1

公开(公告)日：2007-12-27

申请号：PCT/US2007/071680

申请日：2007-06-20

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION ,  PALECEK, Sean, P. ,  MOHR, Jeffrey, C. ,  DEPABLO, Juan, J.

Inventor： PALECEK, Sean, P. ,  MOHR, Jeffrey, C. ,  DEPABLO, Juan, J.

IPC: C12N5/06

CPC classification number: C12N5/0603 ,  C12N2501/115 ,  C12N2502/13 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/10

Abstract: A three-dimensional microwell system that supports long term embryonic stem cell (ESCs) culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured ESCs remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel-coated, tissue culture-treated polystyrene dishes. Microwell-cultured ESCs maintain pluripotency, differentiating to ccach of the three embryonic germ layers. ESC aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain ESC groeth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct ESC differentiation by generating monodisperse EBs of a desired size and shape.

Abstract translation: 描述了支持长期胚胎干细胞（ESCs）培养和形成均质胚状体（EB）的三维微孔系统。 微培养的胚胎干细胞在培养物中保持活性和未分化数周，并保持未分化复制，当传代到基质胶涂覆的组织培养处理的聚苯乙烯培养皿时。 微孔培养的胚胎干细胞保持多能性，与三个胚胎胚层分离。 从微孔释放的ESC聚集体可以传代未分化复制或分化为单分散EB。 以三维方式约束ESC细胞的能力有利地提供了更有效，可再生的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB直接ESC分化的能力。

公开(公告)号：WO2007120302A2

公开(公告)日：2007-10-25

申请号：PCT/US2006/061414

申请日：2006-11-30

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION ,  ABBOTT, Nicholas L. ,  DE PABLO, Juan J. ,  PALECEK, Sean P. ,  LOCKWOOD, Nathan A. ,  MOHR, Jeffrey C. ,  MURPHY, Christopher J. ,  HUANG, Elise

Inventor： ABBOTT, Nicholas L. ,  DE PABLO, Juan J. ,  PALECEK, Sean P. ,  LOCKWOOD, Nathan A. ,  MOHR, Jeffrey C. ,  MURPHY, Christopher J. ,  HUANG, Elise

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  C12N5/0606 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Abstract translation: 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供了用于通过活细胞对细胞外基质组分的变化（例如重组）成像的方法和装置。