公开(公告)号：WO2012086391A1

公开(公告)日：2012-06-28

申请号：PCT/JP2011/078001

申请日：2011-11-28

Applicant: CANON KABUSHIKI KAISHA ,  MURAYAMA, Yohei ,  HASHIMOTO, Hiroyuki ,  KOMATSU, Manabu

Inventor： MURAYAMA, Yohei ,  HASHIMOTO, Hiroyuki ,  KOMATSU, Manabu

IPC: C07K14/475 ,  G01N33/574 ,  G01N33/68

CPC classification number: G01N33/57415 ,  C07K14/4756 ,  G01N33/6848 ,  G01N2333/4756

Abstract: The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500.

Abstract translation: 本发明涉及基于通过蛋白质或肽的质谱法获得的质量信息获取样品中蛋白质或肽的分布信息的信息获取方法。 该方法包括在蛋白质或肽的有限蛋白水解之后样品的确定区域的质谱，并且使用具有Pearson积矩相关系数为0.5的二维强度分布的离子峰获取与分布有关的信息 相对于蛋白质或经受有限蛋白水解的肽的母离子的二维强度分布，确定区域中的1.0和更小，并且具有大于1.0的峰强度比与峰积分的峰强度比 该定子区域中的母离子，其中离子峰的m / z大于500。