公开(公告)号：WO2010116961A1

公开(公告)日：2010-10-14

申请号：PCT/JP2010/056120

申请日：2010-03-30

Applicant: CANON KABUSHIKI KAISHA ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

Inventor： KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC: G01N27/62 ,  G01N33/483 ,  G01N33/68

CPC classification number: G06F19/26 ,  G01N33/6848 ,  H01J49/0004 ,  Y10T436/24

Abstract: An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level. The present invention provides a method of forming a two-dimensional distribution image of a target constituent based on information on the mass of constituents of the tissue section wherein, as the internal standard material, any one of actin, tubulin and GAPDH is used in the intracellular region, one of histone and nucleic acid is used in the nuclear region, and one of albumin and cytokine is used in the extracellular region.

Abstract translation: 本发明的目的是提供一种在细胞水平上全面显示肿瘤组织等中的成分的方法。 本发明提供一种基于组织部位成分质量的信息形成目标成分的二维分布图像的方法，其中作为内标物质，使用肌动蛋白，微管蛋白和GAPDH中的任一种 细胞内区域，组蛋白和核酸之一用于核区域，白蛋白和细胞因子之一用于细胞外区域。

公开(公告)号：WO2005061112A1

公开(公告)日：2005-07-07

申请号：PCT/JP2004/019716

申请日：2004-12-22

Applicant: CANON KABUSHIKI KAISHA ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

Inventor： KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC: B01L3/02

CPC classification number: H01J49/10 ,  B01L3/5085 ,  H01J49/40 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A test specimen is provided which has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances in the range from 1 amol to 1 nmol (excluding the case in which the total quantity is zero).

Abstract translation: 提供了一种试样，其具有在基板上固定为规定的多个独立位置的一种或多种化学物质，并且在各规定位置固定的化学物质的量为为各化学物质定义的存在量单位的整数倍的总和 物质在1〜1nmol范围内（不包括总量为零的情况）。

公开(公告)号：WO2005003715A2

公开(公告)日：2005-01-13

申请号：PCT/JP2004/009788

申请日：2004-07-02

Applicant: CANON KABUSHIKI KAISHA ,  HASHIMOTO, Hiroyuki ,  KUGE, Katsuaki ,  KOMATSU, Manabu ,  NAKAMURA, Kumi ,  BAN, Kazuhiro ,  IMAMURA, Takeshi ,  KOBAYASHI, Shin

Inventor： HASHIMOTO, Hiroyuki ,  KUGE, Katsuaki ,  KOMATSU, Manabu ,  NAKAMURA, Kumi ,  BAN, Kazuhiro ,  IMAMURA, Takeshi ,  KOBAYASHI, Shin

IPC: G01N

CPC classification number: G01N33/6848 ,  H01J49/04 ,  H01J49/40

Abstract: An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.

Abstract translation: 一种用于获取关于目标对象的信息的信息获取方法，包括使用促进目标对象的电离的物质促进目标物体的离子化以使目标对象发射的步骤，以及获取关于物体的信息的步骤的步骤 的飞行目标物体使用飞行时间二次离子质谱法。

公开(公告)号：WO2012096412A1

公开(公告)日：2012-07-19

申请号：PCT/JP2012/051011

申请日：2012-01-12

Applicant: CANON KABUSHIKI KAISHA ,  KOMATSU, Manabu ,  KYOGAKU, Masafumi ,  HASHIMOTO, Hiroyuki ,  AOKI, Naofumi

Inventor： KOMATSU, Manabu ,  KYOGAKU, Masafumi ,  HASHIMOTO, Hiroyuki ,  AOKI, Naofumi

IPC: G01N27/62 ,  G01N27/64

CPC classification number: G01N23/2202 ,  H01J49/0468 ,  H01J49/142

Abstract: Provided is a sample analysis method of irradiating a sample with a primary ion beam to analyze a secondary ion emitted from the sample by mass spectrometry, the sample analysis method including the steps of cooling a sample placed in a chamber; forming an ice layer on a surface of the cooled sample by discharging one of water and an aqueous solution to the chamber; and irradiating the surface of the sample with the primary ion beam with the ice layer being formed thereon, wherein an amount of the water forming the ice layer is 0.1 ng/mm 2 or more and 20 ng/mm 2 or less.

Abstract translation: 提供了一种用一次离子束照射样品以通过质谱分析从样品发射的二次离子的样品分析方法，该样品分析方法包括以下步骤：冷却置于室中的样品; 通过将水和水溶液中的一种排出到所述室，在所述冷却样品的表面上形成冰层; 并且在其上形成有冰层的初级离子束照射样品的表面，其中形成冰层的水的量为0.1ng / mm 2以上且20ng / mm 2以下。

公开(公告)号：WO2011096550A1

公开(公告)日：2011-08-11

申请号：PCT/JP2011/052452

申请日：2011-01-31

Applicant: CANON KABUSHIKI KAISHA ,  TANJI, Koichi ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

Inventor： TANJI, Koichi ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC: G01N27/62

CPC classification number: H01J49/0036 ,  H01J49/0004

Abstract: A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction).

Abstract translation: 提供了更有效的降噪方法。 在该方法中，当处理具有空间分布的质谱信息时，将整个数据作为三维数据（位置信息存储在xy平面中，并且光谱信息沿z轴方向存储），并且三个 通过对频谱方向和峰值分布方向（面内方向）应用优选的基函数来进行维小波噪声降低。

公开(公告)号：WO2005003715A3

公开(公告)日：2005-05-26

申请号：PCT/JP2004009788

申请日：2004-07-02

Applicant: CANON KK ,  HASHIMOTO HIROYUKI ,  KUGE KATSUAKI ,  KOMATSU MANABU ,  NAKAMURA KUMI ,  BAN KAZUHIRO ,  IMAMURA TAKESHI ,  KOBAYASHI SHIN

Inventor： HASHIMOTO HIROYUKI ,  KUGE KATSUAKI ,  KOMATSU MANABU ,  NAKAMURA KUMI ,  BAN KAZUHIRO ,  IMAMURA TAKESHI ,  KOBAYASHI SHIN

IPC: G01N33/68 ,  H01J49/04 ,  H01J49/40

CPC classification number: G01N33/6848 ,  H01J49/04 ,  H01J49/40

Abstract: An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.

Abstract translation: 一种用于获取关于目标对象的信息的信息获取方法，其包括使用促进所述目标对象的电离的物质促进所述目标对象的电离以使所述目标对象发射的步骤以及获取所述质量信息的步骤 的飞行目标物体使用飞行时间二次离子质谱法。

公开(公告)号：WO2012086391A1

公开(公告)日：2012-06-28

申请号：PCT/JP2011/078001

申请日：2011-11-28

Applicant: CANON KABUSHIKI KAISHA ,  MURAYAMA, Yohei ,  HASHIMOTO, Hiroyuki ,  KOMATSU, Manabu

Inventor： MURAYAMA, Yohei ,  HASHIMOTO, Hiroyuki ,  KOMATSU, Manabu

IPC: C07K14/475 ,  G01N33/574 ,  G01N33/68

CPC classification number: G01N33/57415 ,  C07K14/4756 ,  G01N33/6848 ,  G01N2333/4756

Abstract: The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500.

Abstract translation: 本发明涉及基于通过蛋白质或肽的质谱法获得的质量信息获取样品中蛋白质或肽的分布信息的信息获取方法。 该方法包括在蛋白质或肽的有限蛋白水解之后样品的确定区域的质谱，并且使用具有Pearson积矩相关系数为0.5的二维强度分布的离子峰获取与分布有关的信息 相对于蛋白质或经受有限蛋白水解的肽的母离子的二维强度分布，确定区域中的1.0和更小，并且具有大于1.0的峰强度比与峰积分的峰强度比 该定子区域中的母离子，其中离子峰的m / z大于500。

公开(公告)号：WO2012057008A1

公开(公告)日：2012-05-03

申请号：PCT/JP2011/074242

申请日：2011-10-14

Applicant: NIKON CORPORATION ,  KOMATSU, Manabu

Inventor： KOMATSU, Manabu

IPC: G01B11/25

CPC classification number: G01B11/25 ,  Y10T29/49 ,  Y10T29/53022

Abstract: A profile measuring apparatus is provided to be capable of carrying out an optimal measurement for various objects, even provided with a gate -shaped frame structure, with an optical measuring probe. The profile measuring apparatus includes a projection unit which projects a pattern on the object from a projection direction; a measurement unit which takes an image of the pattern from a direction different from the projection direction to measure a position on a surface of the object based on an image data obtained with the taken image; an object - rotation unit which rotates the object in two directions; and a pattern - rotation unit which rotates the pattern.

Abstract translation: 轮廓测量装置被设置成能够利用光学测量探针对具有门形框架结构的各种物体进行最佳测量。 轮廓测量装置包括：投影单元，其从投影方向将图案投影在物体上; 测量单元，其从与所述投影方向不同的方向拍摄所述图案的图像，以基于所获取的图像获得的图像数据来测量所述对象的表面上的位置; 物体旋转单元，其在两个方向上旋转物体; 以及旋转图案的图案旋转单元。

公开(公告)号：WO2011122692A1

公开(公告)日：2011-10-06

申请号：PCT/JP2011/058474

申请日：2011-03-28

Applicant: CANON KABUSHIKI KAISHA ,  NAITO, Jumpei ,  SATOH, Shuya ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

Inventor： NAITO, Jumpei ,  SATOH, Shuya ,  KOMATSU, Manabu ,  HASHIMOTO, Hiroyuki

IPC: G01N27/62 ,  G01N27/64

CPC classification number: G01N33/6851 ,  H01J49/00 ,  Y10T436/182

Abstract: The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.

Abstract translation: 本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。 提供了包含半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后用带电粒子对碱处理的多肽进行解吸，用于质谱法 。