公开(公告)号：WO1993023530A1

公开(公告)日：1993-11-25

申请号：PCT/JP1993000592

申请日：1993-04-30

Applicant: SHIONOGI SEIYAKU KABUSHIKI KAISHA ,  NAKAMURA, Etsuo ,  TSUZUKI, Hiroshige ,  KITADOKORO, Kengo ,  SHIN, Masaru ,  TERAOKA, Hiroshi

Inventor： SHIONOGI SEIYAKU KABUSHIKI KAISHA

IPC: C12N09/52

CPC classification number: C12N9/52 ,  Y10S435/896

Abstract: A novel protease which cleaves the C-terminus of the glutamic acid residue in the amino acid sequence of a polypeptide originating in Streptomyces fradiae, and a process for obtaining the protease from this bacterium. The invention has clarified the properties of this protease and also a DNA sequence coding for this protease and prepropeptides. The protease is specific for glutamic acid and is utilizable for various purposes, such as protein analysis and the cleavage of peptide chains at the desired site of a fused protein.

Abstract translation: 一种新型蛋白酶，其切割源自链霉菌的多肽的氨基酸序列中的谷氨酸残基的C末端，以及从该细菌获得蛋白酶的方法。 本发明已经阐明了该蛋白酶的性质以及编码该蛋白酶和前原肽的DNA序列。 蛋白酶对谷氨酸是特异性的，可用于各种目的，例如蛋白质分析和在融合蛋白的所需位点处的肽链的切割。