公开(公告)号：WO1993023530A1

公开(公告)日：1993-11-25

申请号：PCT/JP1993000592

申请日：1993-04-30

Applicant: SHIONOGI SEIYAKU KABUSHIKI KAISHA ,  NAKAMURA, Etsuo ,  TSUZUKI, Hiroshige ,  KITADOKORO, Kengo ,  SHIN, Masaru ,  TERAOKA, Hiroshi

Inventor： SHIONOGI SEIYAKU KABUSHIKI KAISHA

IPC: C12N09/52

CPC classification number: C12N9/52 ,  Y10S435/896

Abstract: A novel protease which cleaves the C-terminus of the glutamic acid residue in the amino acid sequence of a polypeptide originating in Streptomyces fradiae, and a process for obtaining the protease from this bacterium. The invention has clarified the properties of this protease and also a DNA sequence coding for this protease and prepropeptides. The protease is specific for glutamic acid and is utilizable for various purposes, such as protein analysis and the cleavage of peptide chains at the desired site of a fused protein.

Abstract translation: 一种新型蛋白酶，其切割源自链霉菌的多肽的氨基酸序列中的谷氨酸残基的C末端，以及从该细菌获得蛋白酶的方法。 本发明已经阐明了该蛋白酶的性质以及编码该蛋白酶和前原肽的DNA序列。 蛋白酶对谷氨酸是特异性的，可用于各种目的，例如蛋白质分析和在融合蛋白的所需位点处的肽链的切割。

公开(公告)号：WO01066508A2

公开(公告)日：2001-09-13

申请号：PCT/US2001/007140

申请日：2001-03-06

IPC: B01D9/02 ,  C07C51/43 ,  C07C51/47 ,  C07C59/215 ,  C07C53/02 ,  C07C53/08 ,  C07C53/122 ,  C07C53/124 ,  C07C55/10 ,  C07C57/13 ,  C07C57/145 ,  C07C59/08 ,  C07C59/105 ,  C07C59/19 ,  C07C59/245 ,  C07C59/255 ,  C07C59/265 ,  C07C59/347 ,  C07D307/62

CPC classification number: C07C51/43 ,  Y02P20/582 ,  Y10S435/803 ,  Y10S435/876 ,  Y10S435/896 ,  C07C59/347

Abstract: A process for recovering a desired organic acid from a solution includes the steps of: providing an aqueous solution including at least one desired organic acid or its acid anion; adjusting the proton concentration in the aqueous solution to a desired level, with the desired proton concentration being selected, at least in part, by the amount of available protons needed to associate with the acid anions of the desired organic acid(s) to be recovered and/or acid anions that are weaker than the desired organic acids; and recovering at least a portion of the at least one desired organic acid from the aqueous phase. The desired proton concentration can be based on the amount of available protons being greater than, less than or substantially equal, to the amount of protons needed to associate with the anion of the desired organic acid(s) and acid anions that are weaker than the desired organic acid(s). Specific examples of suitable organic acids include, but are not limited to, ascorbic, succinic, tartaric, glyconic, gulonic, citric, lactic, malic, maleic, acetic, formic, gluconic pyruvic, propionic, butyric, itaconic acids and mixtures thereof. One embodiment of the present invention relates to the recovery of 2-keto-L-gulonic acid (KLG) from aqueous solutions such as fermentation baths.

Abstract translation: 从溶液中回收所需有机酸的方法包括以下步骤：提供包含至少一种所需有机酸或其酸阴离子的水溶液; 将所述水溶液中的质子浓度调节到所需的水平，其中所需质子浓度至少部分地被选择与需要回收的所需有机酸的酸性阴离子缔合所需的可用质子的量 和/或弱于所需有机酸的酸性阴离子; 以及从所述水相中回收所述至少一种所需有机酸的至少一部分。 期望的质子浓度可以基于可用质子的量大于，小于或基本上等于与所需有机酸的阴离子和酸阴离子相比所需的质子的量，其弱于 所需的有机酸。 合适的有机酸的具体实例包括但不限于抗坏血酸，琥珀酸，酒石酸，葡萄糖酸，古洛糖酸，柠檬酸，乳酸，苹果酸，马来酸，乙酸，甲酸，葡糖酸丙酮酸，丙酸，丁酸，衣康酸及其混合物。 本发明的一个实施方案涉及从水溶液如发酵浴中回收2-酮-L-古洛糖酸（KLG）。

公开(公告)号：WO0166508A3

公开(公告)日：2002-05-02

申请号：PCT/US0107140

申请日：2001-03-06

Applicant: EASTMAN CHEM CO

Inventor： COLLINS NICK ALLEN ,  SHELTON MARK ROBERT ,  TINDALL GEORGE WILLIAM ,  PERRI STEVEN THOMAS ,  O'MEADHRA RUAIRI SEOSAMH ,  SINK CHESTER WAYNE ,  ARUMUGAM BHASKAR K ,  HUBBS JOHN CLARK

IPC: B01D9/02 ,  C07C51/43 ,  C07C51/47 ,  C07C59/215 ,  C07C51/48 ,  C07C53/02 ,  C07C53/08 ,  C07C53/122 ,  C07C53/124 ,  C07C55/10 ,  C07C57/13 ,  C07C57/145 ,  C07C59/08 ,  C07C59/105 ,  C07C59/19 ,  C07C59/245 ,  C07C59/255 ,  C07C59/265 ,  C07C59/347 ,  C07D307/62

CPC classification number: C07C51/43 ,  Y02P20/582 ,  Y10S435/803 ,  Y10S435/876 ,  Y10S435/896 ,  C07C59/347

Abstract: A process for recovering a desired organic acid from a solution includes the steps of: providing an aqueous solution including at least one desired organic acid or its acid anion; adjusting the proton concentration in the aqueous solution to a desired level, with the desired proton concentration being selected, at least in part, by the amount of available protons needed to associate with the acid anions of the desired organic acid(s) to be recovered and/or acid anions that are weaker than the desired organic acids; and recovering at least a portion of the at least one desired organic acid from the aqueous phase. The desired proton concentration can be based on the amount of available protons being greater than, less than or substantially equal, to the amount of protons needed to associate with the anion of the desired organic acid(s) and acid anions that are weaker than the desired organic acid(s). Specific examples of suitable organic acids include, but are not limited to, ascorbic, succinic, tartaric, glyconic, gulonic, citric, lactic, malic, maleic, acetic, formic, gluconic pyruvic, propionic, butyric, itaconic acids and mixtures thereof. One embodiment of the present invention relates to the recovery of 2-keto-L-gulonic acid (KLG) from aqueous solutions such as fermentation baths.

Abstract translation: 从溶液中回收所需有机酸的方法包括以下步骤：提供包含至少一种所需有机酸或其酸阴离子的水溶液; 将所述水溶液中的质子浓度调节到所需的水平，其中所需质子浓度至少部分地被选择与需要回收的所需有机酸的酸性阴离子缔合所需的可用质子的量 和/或弱于所需有机酸的酸性阴离子; 以及从所述水相中回收所述至少一种所需有机酸的至少一部分。 期望的质子浓度可以基于可用质子的量大于，小于或基本上等于与所需有机酸的阴离子和酸阴离子相比所需的质子的量，其弱于 所需的有机酸。 合适的有机酸的具体实例包括但不限于抗坏血酸，琥珀酸，酒石酸，葡萄糖酸，古洛糖酸，柠檬酸，乳酸，苹果酸，马来酸，乙酸，甲酸，葡糖酸丙酮酸，丙酸，丁酸，衣康酸及其混合物。 本发明的一个实施方案涉及从水溶液如发酵浴中回收2-酮-L-古洛糖酸（KLG）。

公开(公告)号：WO87007905A1

公开(公告)日：1987-12-30

申请号：PCT/FR1987000233

申请日：1987-12-30

IPC: A23K1/165 ,  A23K1/16 ,  A23K1/18 ,  C12N9/00 ,  C12N9/52 ,  C12P1/04 ,  C12R1/54

CPC classification number: C12N9/52 ,  A23K20/189 ,  A23K50/00 ,  A23K50/30 ,  C12R1/54 ,  Y10S426/801 ,  Y10S426/807 ,  Y10S435/814 ,  Y10S435/896

Abstract: Method for producing a proteolytic complex formed after cultivation of a strain of Streptomyces fradiae while maintaining the pH of the culture medium between 7 and 7.5, and extracting said complex by filtration in the presence of a clarifying agent and ultrafiltration on a specific membrane and finally atomization; the invention also relates to the new proteolytic complex thus obtained and its applications in zootechny due to its stimulating effect on the activity of the pancreas.

Abstract translation: 在培养培养基pH7至7.5之间培养Streptomyces fradiae菌株之后形成蛋白水解复合物的方法，并在澄清剂存在下通过过滤提取所述复合物并在特定膜上超滤，最后雾化 ; 本发明还涉及由此获得的新的蛋白水解复合物及其在动物技术中的应用，因为其对胰腺的活性具有刺激作用。