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公开(公告)号：WO2012129555A2
公开(公告)日：2012-09-27
申请号：PCT/US2012/030479
申请日：2012-03-23
Applicant: BUTAMAX (TM) ADVANCED BIOFUELS LLC , ANTHONY, Larry, Cameron , GIBSON, Katharine, J. , HE, Hongxian , HUANG, Lisa, L. , O'KEEFE, Daniel, P. , KRUCKEBERG, Arthur Leo , LI, Yougen , MAGGIO-HALL, Lori, Ann , MCELVAIN, Jessica , NELSON, Mark, J. , PATNAIK, Ranjan , ROTHMAN, Steven Cary
Inventor： ANTHONY, Larry, Cameron , GIBSON, Katharine, J. , HE, Hongxian , HUANG, Lisa, L. , O'KEEFE, Daniel, P. , KRUCKEBERG, Arthur Leo , LI, Yougen , MAGGIO-HALL, Lori, Ann , MCELVAIN, Jessica , NELSON, Mark, J. , PATNAIK, Ranjan , ROTHMAN, Steven Cary
CPC classification number: C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
Abstract translation: 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。
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公开(公告)号：WO2006069110A3
公开(公告)日：2006-08-31
申请号：PCT/US2005046269
申请日：2005-12-21
Applicant: DU PONT , DICOSIMO ROBERT , PAYNE MARK S , PANOVA ANNA , O'KEEFE DANIEL P , THOMPSON JEFFREY SCOTT
Inventor： DICOSIMO ROBERT , PAYNE MARK S , PANOVA ANNA , O'KEEFE DANIEL P , THOMPSON JEFFREY SCOTT
Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.
Abstract translation: 提供了用于从乙醇腈生产乙醇酸的各种方法。 这些方法包括：1）使用具有改进的腈水解酶活性的乙酰甘氨酸转化成乙醇酸的Acidovorax facilis 72W腈水解酶突变体，和2）提高催化剂稳定性和/或生产率的方法。 提高催化剂稳定性/生产率的方法包括使用反应稳定剂，在基本上无氧条件下进行反应，以及控制反应混合物中底物的浓度。
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公开(公告)号：WO2006023520A3
公开(公告)日：2006-06-15
申请号：PCT/US2005029188
申请日：2005-08-16
Applicant: DU PONT , PAYNE MARK S , DICOSIMO ROBERT , O'KEEFE DANIEL P
Inventor： PAYNE MARK S , DICOSIMO ROBERT , O'KEEFE DANIEL P
Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3 hydroxynitriles to 3 hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3 hydroxynitriles (e.g,. 3 hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3 hydroxycarboxylic acids. A process using the improved mutants to produce the 3-hydroxycarboxylic acids is also provided.
Abstract translation: 本发明涉及具有改进的腈水解酶活性的腈水解酶突变体，用于将3种羟基腈转化为3种羟基羧酸。 更具体地，使用易错PCR和定点诱变使敏捷食酸菌72W（ATCC 55746）腈水解酶基因突变以产生具有改进的腈水解酶活性的腈水解酶，用于将3种羟基腈（例如3羟基丁腈或3-羟基戊腈）转化为 相应的3羟基羧酸。 还提供了使用改进的突变体产生3-羟基羧酸的方法。
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公开(公告)号：WO2009085953A3
公开(公告)日：2009-07-09
申请号：PCT/US2008/087429
申请日：2008-12-18
Applicant: E. I. DU PONT DE NEMOURS AND COMPANY , LI, Yougen , NELSON, Mark, J. , LIAO, Der-Ing , O'KEEFE, Daniel, P.
Inventor： LI, Yougen , NELSON, Mark, J. , LIAO, Der-Ing , O'KEEFE, Daniel, P.
Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
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5.发明申请DIRECT DETECTION METHOD FOR PRODUCTS OF CELLULAR METABOLISM USING TOF-SIMS 审中-公开使用TOF-SIMS的细胞代谢产物的直接检测方法
公开(公告)号：WO2005026692A2
公开(公告)日：2005-03-24
申请号：PCT/US2004/029543
申请日：2004-09-09
Inventor： O'KEEFE, Daniel, P. , LLOYD, Kathryn, G.
IPC: G01N
CPC classification number: C12Q1/02 , G01N23/2258 , G01N33/5005 , G01N33/6848
Abstract: A rapid and efficient method for novel biological substance screening by surface analysis has been developed using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). This method relies on the surface screening of an array of micro-organisms grown on porous membranes, which had previously been in contact with a solid growth medium. ToF-SIMS analysis differentiates among organisms producing different substances, either directly as molecular product, or indirectly through the use of multivariate statistical data reduction techniques. This method has many advantages over traditional microbial screening methods, which require sample preparation and time for assay development.
Abstract translation: 使用飞行时间二次离子质谱（ToF-SIMS）开发了通过表面分析进行新型生物物质筛选的快速有效的方法。 该方法依赖于在先前与固体生长培养基接触的多孔膜上生长的微生物阵列的表面筛选。 ToF-SIMS分析区分产生不同物质的生物，直接作为分子产物，或间接地通过使用多元统计数据减少技术。 该方法与传统的微生物筛选方法相比具有许多优点，需要样品制备和分析开发时间。
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Patent Agency Ranking6.发明申请ALCOHOL DEHYDROGENASES (ADH) USEFUL FOR FERMENTIVE PRODUCTION OF LOWER ALKYL ALCOHOLS 审中-公开醇类脱氢酶（ADH）可用于生产较低级的烷基醇
公开(公告)号：WO2011090753A3
公开(公告)日：2011-10-13
申请号：PCT/US2010062390
申请日：2010-12-29
Applicant: BUTAMAX TM ADVANCED BIOFUELS , SATOGOPAN SRIRIAM , O'KEEFE DANIEL P , GUDE JANARDHAN
Inventor： SATOGOPAN SRIRIAM , O'KEEFE DANIEL P , GUDE JANARDHAN
CPC classification number: C12P7/16 , C12N9/0004 , C12N15/81 , Y02E50/10 , Y02P20/52
Abstract: The invention relates to suitable candidate ADH enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same.
Abstract translation: 本发明涉及用于生产包括异丁醇的低级烷基醇的合适候选ADH酶。 本发明还涉及包含这种ADH酶的重组宿主细胞以及在其中生产低级烷基醇的方法。
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公开(公告)号：WO2009085953A2
公开(公告)日：2009-07-09
申请号：PCT/US2008087429
申请日：2008-12-18
Applicant: DU PONT , LI YOUGEN , NELSON MARK J , LIAO DER-ING , O'KEEFE DANIEL P
Inventor： LI YOUGEN , NELSON MARK J , LIAO DER-ING , O'KEEFE DANIEL P
CPC classification number: C12P7/16 , C12N9/0006 , Y02E50/10
Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
Abstract translation: 提供了NADPH结合酮醇酸还原异构酶获得NADH结合功能性的方法。 描述了从假单胞菌分离出的特定的突变体酮醇酸还原异构酶，其经历了辅因子转换以结合NADH。
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8.发明申请
公开(公告)号：WO2006069114A3
公开(公告)日：2006-10-12
申请号：PCT/US2005046273
申请日：2005-12-21
Applicant: DU PONT , DICOSIMO ROBERT , FOO THOMAS , PAYNE MARK S , PANOVA ANNA , O'KEEFE DANIEL P , THOMPSON JEFFREY SCOTT , GALLAGHER F GLENN , LI XU
Inventor： DICOSIMO ROBERT , FOO THOMAS , PAYNE MARK S , PANOVA ANNA , O'KEEFE DANIEL P , THOMPSON JEFFREY SCOTT , GALLAGHER F GLENN , LI XU
IPC: C12P7/42 , C07C227/26
CPC classification number: C12P7/42
Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
Abstract translation: 提供了一种从甲醛和氰化氢生产乙醇酸的方法。 更具体地说，热处理甲醛和氰化氢反应生成具有低浓度杂质的乙醇腈。 随后使用衍生自敏捷食酸菌72W（ATCC 57746）的具有腈水解酶活性的酶催化剂将乙醇腈转化为乙醇酸铵的水溶液。 使用本文所述的多种方法从羟基乙酸铵水溶液中以酸或盐的形式回收乙醇酸。
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9.发明申请
公开(公告)号：WO2006023520A2
公开(公告)日：2006-03-02
申请号：PCT/US2005/029188
申请日：2005-08-16
Applicant: E.I. DUPONT DE NEMOURS AND COMPANY , PAYNE, Mark, S. , DICOSIMO, Robert , O'KEEFE, Daniel, P.
Inventor： PAYNE, Mark, S. , DICOSIMO, Robert , O'KEEFE, Daniel, P.
Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3 hydroxynitriles to 3 hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3 hydroxynitriles (e.g,. 3 hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3 hydroxycarboxylic acids. A process using the improved mutants to produce the 3-hydroxycarboxylic acids is also provided.
Abstract translation: 本发明涉及将3种羟基腈转化为3种羟基羧酸而具有改善的腈水解酶活性的腈水解酶突变体。 更具体地，使用易错PCR和定点诱变来突变Acidovorax facilis 72W（ATCC 55746）腈水解酶基因，以产生具有改进的腈水解酶活性的腈水解酶，用于将3个羟基腈（例如，3个羟基丁腈或3-羟基戊腈）转化为 相应的3羟基羧酸。 还提供了使用改进的突变体产生3-羟基羧酸的方法。
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公开(公告)号：WO2012129555A3
公开(公告)日：2013-02-28
申请号：PCT/US2012030479
申请日：2012-03-23
Applicant: BUTAMAX TM ADVANCED BIOFUELS , ANTHONY LARRY CAMERON , GIBSON KATHARINE J , HE HONGXIAN , HUANG LISA L , O'KEEFE DANIEL P , KRUCKEBERG ARTHUR LEO , LI YOUGEN , MAGGIO-HALL LORI ANN , MCELVAIN JESSICA , NELSON MARK J , PATNAIK RANJAN , ROTHMAN STEVEN CARY
Inventor： ANTHONY LARRY CAMERON , GIBSON KATHARINE J , HE HONGXIAN , HUANG LISA L , O'KEEFE DANIEL P , KRUCKEBERG ARTHUR LEO , LI YOUGEN , MAGGIO-HALL LORI ANN , MCELVAIN JESSICA , NELSON MARK J , PATNAIK RANJAN , ROTHMAN STEVEN CARY
CPC classification number: C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
Abstract translation: 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。
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