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    POLYMER-BASED PROTEIN ENGINEERING METHODS TO RATIONALLY TUNE ENZYME ACTIVITY, PH-DEPENDENCE AND STABILITY
    2.
    发明申请
    POLYMER-BASED PROTEIN ENGINEERING METHODS TO RATIONALLY TUNE ENZYME ACTIVITY, PH-DEPENDENCE AND STABILITY 审中-公开
    基于聚合物的蛋白质工程方法来显着调节酶活性，PH依赖性和稳定性

    公开(公告)号：WO2014176279A1

    公开(公告)日：2014-10-30

    申请号：PCT/US2014/035033

    申请日：2014-04-22

    Applicant: CARNEGIE MELLON INIVERSITY ,  RUSSELL, Alan, J. ,  KOEPSEL, Richard, R. ,  CUMMINGS, Chad ,  MURATA, Hironobu

    Inventor： RUSSELL, Alan, J. ,  KOEPSEL, Richard, R. ,  CUMMINGS, Chad ,  MURATA, Hironobu

    IPC: A61K47/48

    CPC classification number: A61K47/48176 ,  A61K38/4826 ,  A61K47/48769 ,  A61K47/58 ,  A61K47/69 ,  C08F289/00 ,  C08H1/00 ,  C12Y304/21001

    Abstract: Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.

    Abstract translation: 使用新的具有水溶性的含活性酯酰胺的官能化受控自由基聚合引发剂，已经从蛋白质的表面生长了刺激响应性聚合物，例如糜蛋白酶或具有共价结合活性酯酰胺的表面氨基酸的任何蛋白质 的官能化引发剂。 显示温度或pH的变化可以改变酶周围的聚合物的构象，这反过来使得酶活性和稳定性的合理调整。 这种方法使酶的活性和稳定性提高了一个数量级的pH值，其中酶通常是无活性的或不稳定的。 描述了多峰温度反应性蛋白质 - 嵌段共聚物缀合物。

    ANTIMICROBIAL SURFACES AND METHODS FOR PREPARING ANTIMICROBIAL SURFACES
    6.
    发明申请
    ANTIMICROBIAL SURFACES AND METHODS FOR PREPARING ANTIMICROBIAL SURFACES 审中-公开
    抗微生物表面和制备抗菌表面的方法

    公开(公告)号：WO2005084159A3

    公开(公告)日：2007-05-24

    申请号：PCT/US2004022036

    申请日：2004-07-07

    Applicant: UNIV PITTSBURGH ,  RUSSELL ALAN ,  KOEPSEL RICHARD ,  LEE SANG BEOM ,  MATYJASZEWSKI KRZYSZTOF

    Inventor： RUSSELL ALAN ,  KOEPSEL RICHARD ,  LEE SANG BEOM ,  MATYJASZEWSKI KRZYSZTOF

    IPC: C08F22/30 ,  C08F22/36 ,  C08F122/30 ,  C08F122/36 ,  C08F222/30 ,  C08F222/36

    CPC classification number: A01N33/12 ,  A01N57/34 ,  A61L2/232

    Abstract: The present invention relates to biocidal articles. In an embodiment the biocidal article comprises a plurality of polymers having biocidally active groups. The polymers are attached to a surface and may have a polydispersity less than 3. The biocidally active groups may comprise at least one of a quaternary ammonium salt, a quaternary phosphonium salt or a chloroamine. The attached polymers may be any microstructure, topology or composition, such as, a homopolymer, block copolymer, multiblock copolymer, a random copolymer, graft polymer, a branched or a hyperbranched polymer, and a gradient copolymer. The present invention also comprises a process for the preparation of a biocidal article. Embodiments of the process comprise polymerizing radically polymerizable monomers from an initiator attached to a surface, wherein at least a portion of the monomers comprise a group capable of being converted to a biocidally active group, and converting the group to the biocidally active group.

    Abstract translation: 本发明涉及杀生物制品。 在一个实施方案中，杀生物制品包含多个具有生物活性基团的聚合物。 聚合物连接到表面并且可以具有小于3的多分散性。生物活性基团可以包含季铵盐，季鏻盐或氯胺中的至少一种。 连接的聚合物可以是任何微结构，拓扑结构或组合物，例如均聚物，嵌段共聚物，多嵌段共聚物，无规共聚物，接枝聚合物，支链或超支化聚合物和梯度共聚物。 本发明还包括制备杀生物制品的方法。 该方法的实施方案包括将可自由基聚合的单体从附着于表面的引发剂聚合，其中至少一部分单体包含能够转化为生物活性基团的基团，并将该基团转化为生物活性基团。

    SELF ASSEMBLED NANOTUBES AND METHODS FOR PREPARING THE SAME
    7.
    发明申请
    SELF ASSEMBLED NANOTUBES AND METHODS FOR PREPARING THE SAME 审中-公开

    公开(公告)号：WO2006085896A3

    公开(公告)日：2006-08-17

    申请号：PCT/US2005/016820

    申请日：2005-05-13

    Applicant: NANODYNAMICS, INC. ,  RUSSELL, Alan J. ,  KOEPSEL, Richard R. ,  LEE, Sang Beom

    Inventor： RUSSELL, Alan J. ,  KOEPSEL, Richard R. ,  LEE, Sang Beom

    IPC: B23B5/16 ,  A61K9/14

    Abstract: The invention concerns the synthesis of nanotubes and nanocarpets composed of single chain achiral diacetylenes under conditions where individual molecules form supra molecular associations. Self-assembly is a coordinated association of individual molecules into a defined (usually non-covalent) structure without the intervention of a central controller. Self assembly presumes the ability of a molecule, or group of molecules, to assume a stable or quasi stable structure based solely on the structure of the molecules themselves. The driving force in self-assembling systems is that the supramolecular structure achieves a lower energy state than the individual components alone.

    STABLE THREE ENZYME CREATININE BIOSENSOR
    8.
    发明申请
    STABLE THREE ENZYME CREATININE BIOSENSOR 审中-公开
    稳定的三种酶肌酸生物传感器

    公开(公告)号：WO2006042001A2

    公开(公告)日：2006-04-20

    申请号：PCT/US2005/035959

    申请日：2005-10-05

    Applicant: BAYER HEALTHCARE LLC ,  BERBERICH, Jason ,  BODEN, Mark, W. ,  CHAN, Andy, D., C. ,  RUSSELL, Alan

    Inventor： BERBERICH, Jason ,  BODEN, Mark, W. ,  CHAN, Andy, D., C. ,  RUSSELL, Alan

    CPC classification number: C12Q1/005

    Abstract: The invention provides methods for preparing a stable, multiple-use three enzyme biosensor for the amperometric determination of creatinine in biological liquids that has a useful lifetime that extends significantly beyond that of presently available amperometric biosensors. The biosensor prepared by the methods of the invention encompasses a plurality of immobilized enzymes that are applied to the biosensor as an enzyme-polymer composition. The enzymes, which can include creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, are immobilized into the enzyme-polymer composition simultaneously as well as applied to the biosensor simultaneously. Prior to being immobilized, the enzymes can be chemically modified by attaching one or more polyethylene glycol (PEG) chains per enzyme monomer. The polymer component can be provided by a polyurethane membrane. The invention also provides a method of preparing a biosensor that limits the diffusion of silver ions emanating the reference electrode, thereby preventing, contact between the silver ions and the enzymes. Related methods of preparing an enzyme-polymer composition for incorporation into a multiple use three enzyme biosensor for the amperometric determination of creatinine in biological liquids also are provided. The invention also provides multiple-use biosensors and enzyme-polymer compositions prepared by the methods disclosed.

    Abstract translation: 本发明提供了用于制备用于安培测定生物液体中的肌酸酐的稳定的多次使用的三酶生物传感器的方法，所述生物传感器的使用寿命明显超过目前可用的安培计生物传感器。 通过本发明的方法制备的生物传感器包含多种作为酶 - 聚合物组合物应用于生物传感器的固定化酶。 可以包括肌酸酐酰胺水解酶，肌酸脒基水解酶和肌氨酸氧化酶的酶同时固定在酶 - 聚合物组合物中，并同时应用于生物传感器。 在被固定之前，酶可以通过每个酶单体连接一个或多个聚乙二醇（PEG）链来进行化学修饰。 聚合物组分可以由聚氨酯膜提供。 本发明还提供一种制备生物传感器的方法，该生物传感器限制发射参比电极的银离子的扩散，从而防止银离子与酶之间的接触。 还提供了制备用于并入多用途三酶生物传感器的酶 - 聚合物组合物的相关方法，用于安培测定生物液体中的肌酸酐。 本发明还提供通过所公开的方法制备的多用途生物传感器和酶 - 聚合物组合物。

    ROCK-BOLTING APPARATUS AND METHOD
    9.
    发明申请
    ROCK-BOLTING APPARATUS AND METHOD 审中-公开
    摇动装置和方法

    公开(公告)号：WO2002103162A1

    公开(公告)日：2002-12-27

    申请号：PCT/AU2002/000791

    申请日：2002-06-18

    Applicant: RUSSELL MINERAL EQUIPMENT PTY LIMITED ,  RUBIE, Peter, John ,  RUSSELL, Alan, John

    Inventor： RUBIE, Peter, John ,  RUSSELL, Alan, John

    IPC: E21D20/00

    CPC classification number: E21D11/152 ,  E21B19/087 ,  E21D11/40 ,  E21D20/006

    Abstract: An articulated boom arm for a rock boring machine, comprising a first kinked member having a drill guide at one end and a base pivot at the other end, and a pair of pivoted links. One of said links having a first end pivoted at said base pivot, the other of the links having a drill pivotally mounted at a first end thereof. The second ends of the first and second links being pivoted to each other whereby said drill is reciprocally linearly movable along said first member with a drill bit aligned with said drill guide and at one extremity of the reciprocal movement both said pair of links are substantially longitudinally aligned and extend towards said drill guide, and at the other extremity of the reciprocal movement both said pair of links are substantially longitudinally aligned and extend away from said drill guide.

    Abstract translation: 一种用于岩石钻孔机的铰接起重臂，包括在一端具有钻子引导件的第一扭结构件和在另一端处的基部枢轴以及一对枢转连杆。 所述连杆中的一个具有在所述基部枢轴处枢转的第一端，所述连杆中的另一个具有枢转地安装在其第一端处的钻头。 所述第一和第二连杆的第二端部彼此枢转，由此所述钻具具有与所述钻子引导件对准的钻头沿所述第一构件往复运动，并且在所述往复运动的一个末端处，所述一对链节基本上纵向 对准并延伸朝向所述钻子引导器，并且在所述往复运动的另一个末端处，所述一对连杆基本上纵向对准并且远离所述钻子引导件延伸。

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