公开(公告)号：WO2010126655A1

公开(公告)日：2010-11-04

申请号：PCT/US2010/027417

申请日：2010-03-16

Applicant: THERMO FINNIGAN LLC ,  SENKO, Michael, W.

Inventor： SENKO, Michael, W.

IPC: H01J49/00

CPC classification number: H01J49/427

Abstract: A multi-resolution mass spectrometer system and intra-scanning method is introduced to enhance the measured peak resolution at different regions of a given mass spectrum while not significantly increasing the total duration of the scan. Such an arrangement enables extra resolution where necessary, such as, for example, when incorporating a slower scan rate only over a predetermined narrow low mass marker region of a given mass spectrum. Once past the marker region, the scan rate can be increased to provide the appropriate resolution for peptide identification.

Abstract translation: 引入多分辨率质谱仪系统和扫描内方法，以增强在给定质谱图的不同区域测得的峰分辨率，而不显着增加扫描的总持续时间。 这样的布置可以在必要时实现额外的分辨率，例如当仅在给定质谱的预定的窄的低质量标记区域上结合较慢的扫描速率时。 一旦超过标记区域，可以增加扫描速率以提供适合肽识别的分辨率。

公开(公告)号：WO2010021884A2

公开(公告)日：2010-02-25

申请号：PCT/US2009/053484

申请日：2009-08-11

Applicant: THERMO FINNIGAN LLC ,  SENKO, Michael, W.

Inventor： SENKO, Michael, W.

IPC: H01J49/00

CPC classification number: H01J49/061

Abstract: An ion gate apparatus for controlling the transmission of ion pulses between an origin and a destination in a mass spectrometer is disclosed, comprising: a first split gate having a length L 1 , comprising a first electrode portion; and a second electrode portion electrically insulated from the first electrode portion and separated from the first electrode portion so as to form a first aperture there between; a second split gate disposed adjacent to the first split gate at a distance d from the first split gate and having a length L 2 , comprising a third electrode portion; and a fourth electrode portion electrically insulated from the third electrode portion and separated from the third electrode portion so as to form a second aperture there between; a first voltage source electrically connected to said first electrode portion and to said second electrode portion; a second voltage source electrically connected to said third electrode portion and to said fourth electrode portion; and a controller electrically connected to said first voltage source and to said second voltage source.

Abstract translation: 公开了一种用于控制质谱仪中的原点和目的地之间的离子脉冲的传输的离子门装置，其包括：具有长度L 1的第一分裂栅极， ，包括第一电极部分; 以及第二电极部分，与第一电极部分电绝缘并与第一电极部分分离以在其间形成第一孔径; 第二分离栅极，所述第二分离栅极与所述第一分离栅极相距所述第一分离栅极距离d并且具有长度L 2，所述第二分离栅极包括第三电极部分; 以及与所述第三电极部分电绝缘并与所述第三电极部分分离以在其间形成第二孔的第四电极部分; 电连接到所述第一电极部分和所述第二电极部分的第一电压源; 电连接到所述第三电极部分和所述第四电极部分的第二电压源; 以及电连接到所述第一电压源和所述第二电压源的控制器。

公开(公告)号：WO2007019293A3

公开(公告)日：2007-12-27

申请号：PCT/US2006030405

申请日：2006-08-01

Applicant: THERMO FINNIGAN LLC ,  SENKO MICHAEL W ,  SCHWARTZ JAE C

Inventor： SENKO MICHAEL W ,  SCHWARTZ JAE C

IPC: H01J49/42

CPC classification number: H01J49/4225 ,  H01J49/423

Abstract: An aperture design for a linear ion trap is provided in which the aperture is optimized to minimize possible axial field inhomogeneities whilst preserving the structural integrity of the quadrupole rods. In general, the invention provides a linear ion trap for trapping and subsequently ejecting ions. The linear ion trap comprises a plurality of rods which define an interior trapping volume which has an axis extending longitudinally. One or more of the rods includes an aperture which extends both radially through the rod and longitudinally along the rod. The aperture being configured such that the ions can pass from the interior trapping volume through the aperture to a region outside the interior trapping volume. At least one recess is disposed adjacent the aperture, extending longitudinally along the rod and facing the interior trapping volume, the recess not extending radially through the rod.

Abstract translation: 提供了一种用于线性离子阱的孔径设计，其中孔径被优化以最小化可能的轴向场不均匀性，同时保持四极杆的结构完整性。 通常，本发明提供了用于捕获和随后喷射离子的线性离子阱。 线性离子阱包括多个限定内部捕获体积的杆，其具有纵向延伸的轴线。 杆中的一个或多个包括孔径，其径向延伸穿过杆并沿着杆纵向延伸。 孔被构造成使得离子可以从内部捕获体积通过孔传播到内部捕获体积外部的区域。 至少一个凹部邻近孔设置，沿着杆纵向延伸并且面向内部捕获体积，凹部不径向延伸穿过杆。

公开(公告)号：WO2006130523A1

公开(公告)日：2006-12-07

申请号：PCT/US2006/020628

申请日：2006-05-25

Applicant: THERMO FINNIGAN LLC ,  SENKO, Michael, W. ,  HEMENWAY, Eric ,  HEMENWAY, Tina, A.

Inventor： SENKO, Michael, W. ,  HEMENWAY, Eric ,  HEMENWAY, Tina, A.

IPC: B01D59/44

CPC classification number: H01J49/0036 ,  G01N30/72 ,  G01N30/8603 ,  G01N30/8693

Abstract: A method of analyzing data from a mass spectrometer for a data dependent acquisition is described. In an embodiment of this method, mass spectral scans are taken of a sample eluted from a liquid chromatography column. An extracted ion chromatogram (XIC) is then created for each m/z data point of the mass spectral scans and the XIC for each m/z data point are correlated to a model function, such as a monotonically increasing function, or the first half of a gaussian function, to obtain a XIC correlation value. A weighting function is then applied to the XIC correlation value to obtain a current weighted intensity. The current weighted intensity for each m/z point is used to reconstruct a weighted mass spectrum, which is then used to make a real-time decision for the data dependent acquisition. In an embodiment, the data dependent acquisition is the performance of tandem mass spectrometry.

Abstract translation: 描述了一种从质谱仪分析用于数据相关获取的数据的方法。 在该方法的一个实施方案中，从液相色谱柱洗脱的样品进行质谱扫描。 然后为质谱扫描的每个m / z数据点创建提取的离子色谱图（XIC），并且对于每个m / z数据点的XIC与诸如单调递增函数的模型函数或上半部分 的高斯函数，以获得XIC相关值。 然后将加权函数应用于XIC相关值以获得当前加权强度。 每个m / z点的当前加权强度用于重建加权质谱，然后将其用于对依赖于数据的获取进行实时决策。 在一个实施例中，依赖于数据的获取是串联质谱法的性能。

公开(公告)号：WO2004112084A2

公开(公告)日：2004-12-23

申请号：PCT/US2004016636

申请日：2004-05-25

Applicant: THERMO FINNIGAN LLC ,  SENKO MICHAEL W

Inventor： SENKO MICHAEL W

IPC: H01J49/42 ,  H01J49/00

CPC classification number: H01J49/0063 ,  H01J49/0009 ,  H01J49/422

Abstract: Methods, systems and apparatus, including computer program products, for operating a quadrupole ion trap in mass spectrometry. A calibrated resonant frequency is determined for precursor ions in a first ion population in an ion trap. A frequency adjustment is determined for the precursor ions in a second ion population based on the number of ions in the second ion population. The ion trap is operated using an adjusted resonant frequency that is based on the calibrated resonant frequency and the determined frequency adjustment.

Abstract translation: 用于在质谱中操作四极离子阱的方法，系统和装置，包括计算机程序产品。 在离子阱中的第一离子群中测定前体离子的校准共振频率。 基于第二离子群体中的离子数，确定第二离子群中的前体离子的频率调整。 使用基于校准的谐振频率和所确定的频率调整的调整的谐振频率来操作离子阱。

公开(公告)号：WO2010138120A1

公开(公告)日：2010-12-02

申请号：PCT/US2009/045348

申请日：2009-05-27

Applicant: THERMO FINNIGAN LLC ,  SENKO, Michael, W.

Inventor： SENKO, Michael, W.

IPC: H01J49/00 ,  G01N30/86

CPC classification number: H01J49/0036 ,  G01N30/7233 ,  G01N30/8644 ,  G01N30/8631

Abstract: A method of analyzing data from a mass spectrometer provides data- dependent acquisition. An extracted ion chromatogram (XIC) is created for each m/z data point of mass spectral scans and the XIC for each m/z data point are correlated to a model function to obtain a XIC correlation value. A weighting function is applied to the XIC correlation value to obtain a current weighted intensity for each m/z point, which is used to reconstruct a weighted mass spectrum. The value or range of intensities of interest of the weighted intensity data or raw data is transformed from the time domain into the frequency domain, and the transformed data is used to make a real-time decision for the data-dependent acquisition. The data-dependent acquisition can be the performance of tandem mass spectrometry. A sample processing apparatus receives the sample and a computer readable medium provides instructions to the apparatus.

Abstract translation: 从质谱仪分析数据的方法提供与数据相关的获取。 为质谱扫描的每个m / z数据点创建提取的离子色谱图（XIC），并且将每个m / z数据点的XIC与模型函数相关以获得XIC相关值。 对XIC相关值应用加权函数，以获得用于重建加权质谱的每个m / z点的当前加权强度。 将加权强度数据或原始数据的感兴趣的强度的值或范围从时域变换到频域，并且使用变换后的数据进行数据相关采集的实时决策。 数据相关获取可以是串联质谱的性能。 样品处理装置接收样品，并且计算机可读介质向装置提供指令。

公开(公告)号：WO2010021884A3

公开(公告)日：2010-06-10

申请号：PCT/US2009053484

申请日：2009-08-11

Applicant: THERMO FINNIGAN LLC ,  SENKO MICHAEL W

Inventor： SENKO MICHAEL W

IPC: H01J3/26 ,  H01J49/06

CPC classification number: H01J49/061

Abstract: An ion gate apparatus for controlling the transmission of ion pulses between an origin and a destination in a mass spectrometer is disclosed, comprising: a first split gate having a length L1, comprising a first electrode portion; and a second electrode portion electrically insulated from the first electrode portion and separated from the first electrode portion so as to form a first aperture there between; a second split gate disposed adjacent to the first split gate at a distance d from the first split gate and having a length L2, comprising a third electrode portion; and a fourth electrode portion electrically insulated from the third electrode portion and separated from the third electrode portion so as to form a second aperture there between; a first voltage source electrically connected to said first electrode portion and to said second electrode portion; a second voltage source electrically connected to said third electrode portion and to said fourth electrode portion; and a controller electrically connected to said first voltage source and to said second voltage source.

Abstract translation: 公开了一种用于控制在质谱仪中的原点和目的地之间的离子脉冲的透射的离子门装置，包括：具有长度L1的第一分裂门，包括第一电极部分; 以及与所述第一电极部分电绝缘并与所述第一电极部分分离以在其间形成第一孔的第二电极部分; 第二分离栅极，与第一分离栅极相邻设置，距离第一分离栅极距离为d，具有长度L2，包括第三电极部分; 以及与所述第三电极部分电绝缘并与所述第三电极部分分离以在其间形成第二孔的第四电极部分; 电连接到所述第一电极部分和所述第二电极部分的第一电压源; 电连接到所述第三电极部分和所述第四电极部分的第二电压源; 以及电连接到所述第一电压源和所述第二电压源的控制器。

公开(公告)号：WO2009012063A3

公开(公告)日：2009-12-10

申请号：PCT/US2008069083

申请日：2008-07-02

Applicant: THERMO FINNIGAN LLC ,  SENKO MICHAEL W

Inventor： SENKO MICHAEL W

IPC: H01J49/42

CPC classification number: H01J49/004 ,  H01J49/38 ,  H01J49/422 ,  H01J49/427

Abstract: The present invention pertains to a method and apparatus which increases the efficiency with which ions are transported from a first ion trap to a second ion trap, and subsequently trapped in the second ion trap. In one aspect the invention, increased efficiency takes the form or enabling ions of both high and low mass to charge ratios to be trapped in the second ion trap at substantially the same time, or at least within a relatively small window of time. This can be achieved by minimizing the undesirable time-of-flight separation by the high and low mass to charge ratio ions as they are transported from a first ion trap to the second ion trap. This minimization can be realized by adjusting the potential energy applied to ion transfer optics disposed between the two ion traps.

Abstract translation: 本发明涉及提高离子从第一离子阱传递到第二离子阱的效率的方法和装置，并随后被捕获在第二离子阱中。 在一个方面，本发明提供的效率使得高质量和低质量的电荷离子的形式或使得能够在基本上相同的时间或至少在相对较小的时间窗内被捕获在第二离子阱中。 这可以通过将高质量和低质量与电荷比的离子在从第一离子阱传输到第二离子阱时最小化不期望的飞行时间分离来实现。 这种最小化可以通过调节施加到设置在两个离子阱之间的离子传递光学器件的势能来实现。

公开(公告)号：WO2009012063A2

公开(公告)日：2009-01-22

申请号：PCT/US2008/069083

申请日：2008-07-02

Applicant: THERMO FINNIGAN LLC ,  SENKO, Michael, W.

Inventor： SENKO, Michael, W.

CPC classification number: H01J49/004 ,  H01J49/38 ,  H01J49/422 ,  H01J49/427

Abstract: The present invention pertains to a method and apparatus which increases the efficiency with which ions are transported from a first ion trap to a second ion trap, and subsequently trapped in the second ion trap. In one aspect the invention, increased efficiency takes the form or enabling ions of both high and low mass to charge ratios to be trapped in the second ion trap at substantially the same time, or at least within a relatively small window of time. This can be achieved by minimizing the undesirable time-of-flight separation by the high and low mass to charge ratio ions as they are transported from a first ion trap to the second ion trap. This minimization can be realized by adjusting the potential energy applied to ion transfer optics disposed between the two ion traps.

Abstract translation: 本发明涉及提高离子从第一离子阱转移到第二离子阱并随后捕获在第二离子阱中的效率的方法和设备。 在本发明的一个方面中，增加的效率采用形式或使得高质量与低质量比的离子基本上同时或至少在相对小的时间窗内被捕获在第二离子阱中。 这可以通过在离子从第一离子阱转移到第二离子阱时由高和低质荷比离子使不希望的飞行时间分离最小化来实现。 这种最小化可以通过调整施加到布置在两个离子阱之间的离子转移光学器件的势能来实现。

公开(公告)号：WO2008098081A2

公开(公告)日：2008-08-14

申请号：PCT/US2008/053219

申请日：2008-02-06

Applicant: THERMO FINNIGAN LLC ,  SENKO, Michael, W.

Inventor： SENKO, Michael, W.

CPC classification number: H01J49/423 ,  H01J49/004

Abstract: A tandem mass spectrometer includes a two-dimensional ion trap that has an elongated ion-trapping region extending along a continuously curving path between first and second opposite ends thereof. The elongated trapping region has a central axis that is defined substantially parallel to the curved path and that extends between the first and second opposite ends. The two-dimensional ion trap is configured for receiving ions through the first end and for mass selectively ejecting the ions along a direction that is orthogonal to the central axis, such that the ejected ions are directed generally toward a common point. The tandem mass spectrometer also includes a collision cell having an ion inlet that is disposed about the common point for receiving the ions that are ejected therefrom and for causing at least a portion of the ions to undergo collisions and form product ions by fragmentation. A mass analyzer in communication with the collision cell receives the product ions from the collision cell and obtains product ion mass spectra with a rapid scan rate, hi this way, a plurality of product ion spectra may be obtained for a large number of precursor ions in a sample without the need for data-dependent operation.

Abstract translation: 串联质谱仪包括二维离子阱，其具有在其第一和第二相对端之间沿着连续弯曲路径延伸的细长离子捕获区域。 细长捕获区域具有中心轴线，该中心轴线基本上平行于弯曲路径限定，并且在第一和第二相对端部之间延伸。 二维离子阱被配置用于通过第一端接收离子，并且用于沿着与中心轴正交的方向大量选择性地排出离子，使得所排出的离子大致指向公共点。 串联质谱仪还包括具有离子入口的碰撞池，该离子入口围绕公共点设置以接收从其喷出的离子，并使至少一部分离子发生碰撞并通过碎裂形成产物离子。 与碰撞单元通信的质量分析器接收来自碰撞池的产物离子，并且以快速扫描速率获得产物离子质谱。通过这种方式，可以获得大量前体离子的多个产物离子光谱 一个样本，而不需要依赖于数据的操作。