METHOD FOR PREPARING DIOL COMPOUND AT HIGH YIELD USING GLYCEROL FERMENTATION MICROORGANISM VARIANT
    1.
    发明申请
    METHOD FOR PREPARING DIOL COMPOUND AT HIGH YIELD USING GLYCEROL FERMENTATION MICROORGANISM VARIANT 审中-公开
    利用甘油发酵微生物变异体以高收率制备二醇化合物的方法

    公开(公告)号:WO2013012293A9

    公开(公告)日:2013-05-16

    申请号:PCT/KR2012005832

    申请日:2012-07-20

    CPC classification number: C12N1/32 C12N9/0006 C12P7/18 C12Y101/01027

    Abstract: The present invention relates to a method for preparing a diol compound from glycerol using a variant strain, and more specifically to a method for producing 1,3-propanediol and 2,3-butanediol at a high yield by using a variant of a microorganism capable of fermenting glycerol that lacks a gene for coding lactate dehydrogenase. According to the present invention, the production amount of 1,3-propanediol and 2,3-butanediol can be significantly improved through a novel culturing method of a glycerol fermentation microorganism variant.

    Abstract translation: 本发明涉及使用变异株由甘油制备二醇化合物的方法,更具体地涉及通过使用能够以高产率生产1,3-丙二醇和2,3-丁二醇的微生物的变体以高产率生产1,3-丙二醇和2,3-丁二醇的方法 发酵甘油缺乏编码乳酸脱氢酶的基因。 根据本发明,通过甘油发酵微生物变体的新型培养方法,可以显着提高1,3-丙二醇和2,3-丁二醇的生产量。

    MUTANT MICROORGANISM HAVING HIGH ETHANOL GENERATION ABILITY FROM GLYCEROL OR CRUDE GLYCEROL, AND METHOD FOR PRODUCING ETHANOL USING SAME
    2.
    发明申请
    MUTANT MICROORGANISM HAVING HIGH ETHANOL GENERATION ABILITY FROM GLYCEROL OR CRUDE GLYCEROL, AND METHOD FOR PRODUCING ETHANOL USING SAME 审中-公开
    具有来自甘油或粗甘油的高乙醇产生能力的突变微生物,以及使用相同方法生产乙醇的方法

    公开(公告)号:WO2012030195A3

    公开(公告)日:2012-05-31

    申请号:PCT/KR2011006536

    申请日:2011-09-02

    Abstract: The present invention relates to a mutant microorganism having enhanced capacity for generating ethanol using glycerol or crude glycerol as a carbon source, and to a method for producing ethanol using same. More particularly, the present invention relates to a mutant microorganism having high ethanol generation ability from glycerol or crude glycerol, wherein the enzymatic activity of alcohol dehydrogenase (AdhII) is increased and the enzymatic activity of 1,3-propanediol oxidoreductase (DhaT) is decreased in the mutant organism. The present invention also relates to a microorganism improved by means of the aforementioned mutation, and to a method for producing ethanol using the mutant microorganism.

    Abstract translation: 本发明涉及使用甘油或粗甘油作为碳源来提高乙醇产生能力的突变微生物以及使用该微生物生产乙醇的方法。 更具体地,本发明涉及甘油或粗甘油具有高乙醇生成能力的突变微生物,其中乙醇脱氢酶(AdhII)的酶活性增加并且1,3-丙二醇氧化还原酶(DhaT)的酶活性降低 在突变生物体中。 本发明还涉及通过上述突变改进的微生物,以及使用该突变微生物生产乙醇的方法。

    NOVEL THRAUSTOCHYTRID-BASED MICROALGAE, AND METHOD FOR PREPARING BIO-OIL BY USING SAME
    4.
    发明申请
    NOVEL THRAUSTOCHYTRID-BASED MICROALGAE, AND METHOD FOR PREPARING BIO-OIL BY USING SAME 审中-公开
    基于新戊酰基的微乳液,以及使用它们制备生物油的方法

    公开(公告)号:WO2011139040A3

    公开(公告)日:2012-05-18

    申请号:PCT/KR2011002999

    申请日:2011-04-25

    CPC classification number: C12N1/12 C12P7/6463 C12P7/6472 C12R1/89

    Abstract: The present invention relates to novel thraustochytrid-based microalgae capable of producing bio-oil, and a method for preparing bio-oil by using the same. The microalgae according to the present invention can produce bio-oil at a high yield by accumulating bio-oil in strains at a high ratio when cultured in a medium containing glucose and can produce bio-oil by using a bean powder as a nitrogen source, and a cultured product can be used as a material for preparing food and feed by using an edible bean powder as a medium. In addition, the microalgae according to the present invention can produce bio-oil by using non-edible fibrous biomass as a carbon source, and it is possible to overcome the limits of the development of biofuel such as a food resource supply and demand imbalance, price increase of raw materials and the like and to ensure industrial competitiveness of microbial fermented oils by using non-edible fibrous biomass as a medium.

    Abstract translation: 本发明涉及能够生产生物油的新型基于thraustochytrid的微藻类,以及使用该生物油制备生物油的方法。 根据本发明的微藻可以通过在含有葡萄糖的培养基中培养时以高比率积累生物油而以高产率生产生物油,并且可以通过使用豆粉作为氮源来生产生物油, 可以使用可食用的豆粉作为介质,将培养的产品用作食品和饲料的制备材料。 此外,根据本发明的微藻可以通过使用不可食用的纤维生物质作为碳源来生产生物油,并且可以克服生物燃料的发展的限制,例如食物供应和需求不平衡, 原材料价格上涨等,并通过使用不可食用的纤维生物质作为介质来确保微生物发酵油的工业竞争力。

    MUTANT MICROORGANISM HAVING HIGH ETHANOL GENERATION ABILITY FROM GLYCEROL OR CRUDE GLYCEROL, AND METHOD FOR PRODUCING ETHANOL USING SAME
    6.
    发明申请
    MUTANT MICROORGANISM HAVING HIGH ETHANOL GENERATION ABILITY FROM GLYCEROL OR CRUDE GLYCEROL, AND METHOD FOR PRODUCING ETHANOL USING SAME 审中-公开
    具有来自甘草酸或甘草酸的高乙醇产生能力的突变微生物以及使用其制备乙醇的方法

    公开(公告)号:WO2012030195A2

    公开(公告)日:2012-03-08

    申请号:PCT/KR2011006536

    申请日:2011-09-02

    Abstract: The present invention relates to a mutant microorganism having enhanced capacity for generating ethanol using glycerol or crude glycerol as a carbon source, and to a method for producing ethanol using same. More particularly, the present invention relates to a mutant microorganism having high ethanol generation ability from glycerol or crude glycerol, wherein the enzymatic activity of alcohol dehydrogenase (AdhII) is increased and the enzymatic activity of 1,3-propanediol oxidoreductase (DhaT) is decreased in the mutant organism. The present invention also relates to a microorganism improved by means of the aforementioned mutation, and to a method for producing ethanol using the mutant microorganism.

    Abstract translation: 本发明涉及使用甘油或粗甘油作为碳源产生乙醇的能力增强的突变体微生物,以及使用该乙醇生产乙醇的方法。 更具体地说,本发明涉及从甘油或粗甘油具有高乙醇生成能力的突变体微生物,其中醇脱氢酶(AdhII)的酶活性提高,1,3-丙二醇氧化还原酶(DhaT)的酶活性降低 在突变体中。 本发明还涉及通过上述突变而改善的微生物,以及使用该突变型微生物的乙醇的制造方法。

    METHOD FOR PREPARING 3-HYDROXYPROPIONIC ACID FROM GLYCEROL IN HIGH YIELD
    7.
    发明申请
    METHOD FOR PREPARING 3-HYDROXYPROPIONIC ACID FROM GLYCEROL IN HIGH YIELD 审中-公开
    从高甘油三酸制备3-羟基丙酸的方法

    公开(公告)号:WO2011149248A3

    公开(公告)日:2012-03-08

    申请号:PCT/KR2011003798

    申请日:2011-05-24

    CPC classification number: C12N15/746 C12N1/32 C12N15/52 C12P7/42 C12R1/22

    Abstract: The present invention relates to a method for producing 3-hydroxypropionic acid by cultivating a mutated microorganism in a medium containing glycerol, wherein a gene encoding a protein which uses propanediol is inserted into and amplified in the mutated microorganism which is then capable of producing coenzyme B12 and 3-hydroxypropionic acid using glycerol as a carbon source. The present invention enables the preparation of 3-hydroxypropionic acid in high yield from glycerol without having to add expensive coenzyme B12 as a cofactor.

    Abstract translation: 本发明涉及通过在含有甘油的培养基中培养突变的微生物来生产3-羟基丙酸的方法,其中将编码使用丙二醇的蛋白质的基因插入突变的微生物中扩增,然后扩增其能够产生辅酶B12 和使用甘油作为碳源的3-羟基丙酸。 本发明能够以甘油高产率制备3-羟基丙酸,而不需要加入昂贵的辅酶B12作为辅因子。

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