公开(公告)号：WO2010101839A2

公开(公告)日：2010-09-10

申请号：PCT/US2010/025791

申请日：2010-03-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  JUO, Rouh-Rong ,  BROOKS, Edward ,  GEE, Melissa ,  WANG, Zhixian H ,  SKAARE, Kathleen

Inventor： JUO, Rouh-Rong ,  BROOKS, Edward ,  GEE, Melissa ,  WANG, Zhixian H ,  SKAARE, Kathleen

IPC: C07D321/00 ,  C07D407/04

CPC classification number: C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

Abstract: Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: 0-0 ΛR R R T (i) where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron- withdrawing groups or or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein Ri is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

Abstract translation: 描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。 进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地为任何支链烷基或环烷基，其为二氧杂环丁烷提供稳定性，或者两个R基团一起形成环烷基或聚环烷基部分螺 二环氧环乙烷环，其中每个R基团或螺环结合的部分可以是未取代的或被一个或多个吸电子基团或供电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R 1是芳基或 1-20个碳原子的烷基，其可任选被1个或多个卤素原子取代，并且其中T是能够在二氧杂环丁烷I的酶活化分解时能发光的芳基或杂芳基环。试剂盒，方法和试验也是 公开了包含二恶丁环化合物。

公开(公告)号：WO2010101839A3

公开(公告)日：2011-01-13

申请号：PCT/US2010025791

申请日：2010-03-01

Applicant: LIFE TECHNOLOGIES CORP ,  JUO ROUH-RONG ,  BROOKS EDWARD ,  GEE MELISSA ,  WANG ZHIXIAN H ,  SKAARE KATHLEEN

Inventor： JUO ROUH-RONG ,  BROOKS EDWARD ,  GEE MELISSA ,  WANG ZHIXIAN H ,  SKAARE KATHLEEN

IPC: C07D321/00 ,  C07D407/04

CPC classification number: C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

Abstract: Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: 0-0 ?R R R T (i) where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron- withdrawing groups or or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein Ri is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

Abstract translation: 描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。 进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地为任何支链烷基或环烷基，其为二氧杂环丁烷提供稳定性，或者两个R基团一起形成环烷基或聚环烷基部分螺 二环氧环乙烷环，其中每个R基团或螺环结合的部分可以是未取代的或被一个或多个吸电子基团或供电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R 1是芳基或 1-20个碳原子的烷基，其可任选被1个或多个卤素原子取代，并且其中T是能够在二氧杂环丁烷I的酶活化分解时能发光的芳基或杂芳基环。试剂盒，方法和试验也是 公开了包含二恶丁环化合物。