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222 条记录 (耗时 0.041 秒)

    METHODS AND SYSTEM FOR DETECTING MELANOMA
    3.
    发明申请
    METHODS AND SYSTEM FOR DETECTING MELANOMA 审中-公开
    检测梅毒的方法和系统

    公开(公告)号：WO2013179293A1

    公开(公告)日：2013-12-05

    申请号：PCT/IL2013/050467

    申请日：2013-05-30

    申请人： RAMOT AT TEL-AVIV UNIVERSITY LTD.

    发明人： VERNICK, Sefi ,  SHACHAM-DIAMAND, Yosi ,  RISHPON, Judith ,  MOSSBERG, Michal ,  VAISMAN, Ofri

    IPC分类号： C12Q1/26 ,  G01N27/403

    CPC分类号： G01N33/5743 ,  C12Q1/26 ,  G01N27/327 ,  G01N33/5011 ,  G01N2333/90245 ,  G01N2800/52

    摘要： A method of determining tyrosinase activity of a cell is disclosed. The method comprises: (a) contacting the cell with a phenol under conditions wherein tyrosinase of the cell catalyzes a reaction with said phenol, so as to generate a product which produces an electrical signal; and (b) measuring a level of the electrical signal, thereby determining tyrosinase activity of the cell. Use of the method for detecting melanoma in skin samples is also disclosed.

    摘要翻译： 公开了确定细胞酪氨酸酶活性的方法。 该方法包括：（a）在细胞酪氨酸酶催化与所述苯酚反应的条件下使细胞与苯酚接触，以产生产生电信号的产物; 和（b）测量电信号的水平，从而确定细胞的酪氨酸酶活性。 还公开了在皮肤样品中检测黑素瘤的方法。

    METHODS AND SYSTEMS FOR EVALUATING AND PREDICTING THE REACTIVITY OF MONOOXYGENASE ENZYMES
    4.
    发明申请
    METHODS AND SYSTEMS FOR EVALUATING AND PREDICTING THE REACTIVITY OF MONOOXYGENASE ENZYMES 审中-公开
    评价和预测单核苷酸酶活性的方法与系统

    公开(公告)号：WO2012109586A3

    公开(公告)日：2013-01-24

    申请号：PCT/US2012024723

    申请日：2012-02-10

    申请人： UNIV ROCHESTER ,  FASAN RUDI ,  ZHANG KAIDONG

    发明人： FASAN RUDI ,  ZHANG KAIDONG

    IPC分类号： C12Q1/26 ,  C07C43/03 ,  C12N9/02

    CPC分类号： C12Q1/26 ,  C12N15/102 ,  C12N15/1093 ,  C40B30/08 ,  G01N2333/90241 ,  G01N2333/90245

    摘要： Methods and systems for evaluating and predicting the reactivity of natural and engineered monooxygenase enzymes are provided. Methods are provided for acquiring a functional profile (fingerprint) of monooxygenases that encode information regarding the active site configuration of such monooxygenases. Methods are also provided for carrying out analysis of a monooxygenase fingerprint, to formulate predictions regarding the reactivity properties (e.g., substrate reactivity, chemo-, regio, and stereoselectivity properties) of the fingerprinted monooxygenases.

    摘要翻译： 提供了用于评估和预测天然和工程化单加氧酶的反应性的方法和系统。 提供了用于获取编码关于这种单加氧酶的活性位点构型的信息的单加氧酶的功能谱（指纹图谱）的方法。 还提供了用于进行单加氧酶指纹分析的方法，以制定关于指纹图谱的单加氧酶的反应性（例如底物反应性，化学，区域和立体选择性）的预测。

    TMEM195 ENCODES FOR TETRAHYDROBIOPTERIN-DEPENDENT ALKYLGLYCEROL MONOOXYGENASE ACTIVITY
    5.
    发明申请
    TMEM195 ENCODES FOR TETRAHYDROBIOPTERIN-DEPENDENT ALKYLGLYCEROL MONOOXYGENASE ACTIVITY 审中-公开
    TMEM195四氢叶酸酯依赖性烷基胆甾醇单核苷酸活性的编码

    公开(公告)号：WO2011131626A1

    公开(公告)日：2011-10-27

    申请号：PCT/EP2011/056142

    申请日：2011-04-18

    申请人： MEDIZINISCHE UNIVERSITÄT INNSBRUCK ,  WERNER, Ernst R. ,  WATSCHINGER, Katrin

    发明人： WERNER, Ernst R.

    IPC分类号： C12N9/02 ,  A61K38/17 ,  A61K38/44

    CPC分类号： C12N9/0071 ,  A61K38/00 ,  C12Q1/26 ,  G01N2333/90245 ,  G01N2500/10 ,  G01N2800/2821

    摘要： The present invention relates to the provision of a pharmaceutical composition comprising a nucleic acid molecule encoding a alkylglycerol monooxygenase (TMEM195; glyceryl ether monooxygenase; EC 1.14.16.5). The present invention also provides for a method for producing said alkylglycerol monooxygenase (TMEM195; glyceryl ether monooxygenase; EC 1.14.16.5) polypeptides encoded by said polynucleotides. Moreover, the use of such polypeptides as well as of antagonists/inhibitors of such polypeptides in a medical setting (e.g. in from of a pharmaceutical composition) and methods for assessing the activity of a candidate molecule suspected of being an antagonist/inhibitor or agonist/activator in order to identify potential antagonists/inhibitors or agonists/activators of the polypeptide are also provided in the present invention. Finally, the present invention provides kits for carrying out said methods wherein the kits comprise polynucleotides and/or antibodies capable of detecting the activity of alkylglycerol monooxygenase.

    摘要翻译： 本发明涉及提供包含编码烷基甘油单加氧酶（TMEM195;甘油醚单加氧酶; EC 1.14.16.5）的核酸分子的药物组合物。 本发明还提供了用于制备由所述多核苷酸编码的所述烷基甘油单加氧酶（TMEM195;甘油醚单加氧酶; EC 1.14.16.5）多肽的方法。 此外，这种多肽以及这种多肽的拮抗剂/抑制剂在医疗环境中的用途（例如从药物组合物中的应用）以及用于评估疑似是拮抗剂/抑制剂或激动剂/拮抗剂的候选分子的活性的方法， 为了鉴定潜在的拮抗剂/抑制剂或多肽的激动剂/活化剂，也在本发明中提供。 最后，本发明提供了用于进行所述方法的试剂盒，其中试剂盒包含能够检测烷基甘油单加氧酶活性的多核苷酸和/或抗体。

    CHEMILUMINESCENT COMPOSITIONS, METHODS, ASSAYS AND KITS FOR OXIDATIVE ENZYMES
    7.
    发明申请
    CHEMILUMINESCENT COMPOSITIONS, METHODS, ASSAYS AND KITS FOR OXIDATIVE ENZYMES 审中-公开
    氧化酶的显色组合物，方法，测定和试剂

    公开(公告)号：WO2010101839A3

    公开(公告)日：2011-01-13

    申请号：PCT/US2010025791

    申请日：2010-03-01

    申请人： LIFE TECHNOLOGIES CORP ,  JUO ROUH-RONG ,  BROOKS EDWARD ,  GEE MELISSA ,  WANG ZHIXIAN H ,  SKAARE KATHLEEN

    发明人： JUO ROUH-RONG ,  BROOKS EDWARD ,  GEE MELISSA ,  WANG ZHIXIAN H ,  SKAARE KATHLEEN

    IPC分类号： C07D321/00 ,  C07D407/04

    CPC分类号： C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

    摘要： Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: 0-0 ?R R R T (i) where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron- withdrawing groups or or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein Ri is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

    摘要翻译： 描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。 进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地为任何支链烷基或环烷基，其为二氧杂环丁烷提供稳定性，或者两个R基团一起形成环烷基或聚环烷基部分螺 二环氧环乙烷环，其中每个R基团或螺环结合的部分可以是未取代的或被一个或多个吸电子基团或供电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R 1是芳基或 1-20个碳原子的烷基，其可任选被1个或多个卤素原子取代，并且其中T是能够在二氧杂环丁烷I的酶活化分解时能发光的芳基或杂芳基环。试剂盒，方法和试验也是 公开了包含二恶丁环化合物。

    CALMODULIN INDEPENDENT ACTIVATION OF NITRIC OXIDE SYNTHASE
    9.
    发明申请
    CALMODULIN INDEPENDENT ACTIVATION OF NITRIC OXIDE SYNTHASE 审中-公开
    硝酸氧化酶合成酶的钙调素独立活化

    公开(公告)号：WO2005115407A3

    公开(公告)日：2009-04-09

    申请号：PCT/US2005018379

    申请日：2005-05-25

    申请人： RENSSELAER POLYTECH INST ,  SALERNO JOHN C ,  SMITH SUSAN M E

    发明人： SALERNO JOHN C ,  SMITH SUSAN M E

    IPC分类号： A61K31/675 ,  A61K31/7052

    CPC分类号： A61K31/7052 ,  A61K31/675 ,  G01N2333/90245 ,  G01N2500/02

    摘要： Methods for activation of a constitutive nitric oxide synthase are described, as are agents which activate a constitutive nitric oxide synthase (e.g., NADPH analogs), methods of identifying such agents, and methods of treatment of diseases or conditions associated with nitric oxide production by a constitutive nitric oxide synthase, by administering an agent which activates the constitutive nitric oxide synthase.

    摘要翻译： 描述了激活组成型一氧化氮合成酶的方法，活化组成型一氧化氮合酶（例如NADPH类似物）的试剂，鉴定这些试剂的方法，以及治疗与一氧化氮产生有关的疾病或病症的方法 通过施用激活组成型一氧化氮合酶的试剂来构成一氧化氮合酶。