公开(公告)号：WO2019014317A1

公开(公告)日：2019-01-17

申请号：PCT/US2018/041591

申请日：2018-07-11

Applicant: ROBERT BOSCH GMBH ,  SHIN, Young, Shik ,  FOMINA, Nadezda ,  JOHNSON, Christopher ,  LANG, Christoph ,  STALEY, Patrick

Inventor： SHIN, Young, Shik ,  FOMINA, Nadezda ,  JOHNSON, Christopher ,  LANG, Christoph ,  STALEY, Patrick

IPC: G01N33/543 ,  G01N33/58 ,  G01N27/327

Abstract: A method for detecting a presence and/or a concentration of a target substance in a reagent solution using enzyme-linked immunosorbent assay (ELIS A) includes binding the target substance directly or indirectly to an electrode, and binding a detection agent directly or indirectly to the bound target substance. The method further includes modulating a pH of only a portion of the reagent solution in which the bound target substance and the bound detection agent are located using the electrode, the modulated pH of the portion of the reagent solution causing the bound detection agent to undergo a change, and detecting the change in the bound detection agent. The detected change corresponds to the presence of the target substance in the reagent solution and/or the concentration of the target substance in the reagent solution.