公开(公告)号：WO1994002618A1

公开(公告)日：1994-02-03

申请号：PCT/EP1993001917

申请日：1993-07-19

Applicant: GIST-BROCADES N.V. ,  MULLENERS, Leonardus, Johannes, Sofie, Marie ,  MISSET, Onno ,  VAN DER LAAN, Jan, Metske ,  VAN GASTEL, Franciscus, Josephus, Cornelius ,  BROEKHUIZEN, Cornelis, Petrus ,  BAAS, Erik, Jan

Inventor： GIST-BROCADES N.V.

IPC: C12N15/57

CPC classification number: C11D3/386 ,  C12N9/54

Abstract: New PB92 or Subtilisin 309 mutant serine proteases are provided having specific mutations, resulting in a surprisingly better wash performance or in an improved storage stability with at similar or even better wash performance. These PB92 or Subtilisin 309 mutants include mutations at positions 60, 87, 97, 99, 102, 116, 117, 126, 127, 128, 130, 133, 134, 154, 156, 158, 159, 160, 164, 169, 175, 180, 182, 193, 197, 198, 203, 211, and 216. The new proteases, therefore, are very suitable for use in various types of detergents, whether or not in conjunction with other enzymes, for example amylases, cellulases and lipases. Preferred embodiments are the PB92 and Subtilisin 309 mutants having a mutation at position 102 and in particular those having at least one further mutation.

Abstract translation: 提供新的PB92或枯草杆菌蛋白酶309突变丝氨酸蛋白酶，其具有特异性突变，导致惊人的更好的洗涤性能或者在相似或甚至更好的洗涤性能下具有改善的储存稳定性。 这些PB92或枯草杆菌蛋白酶309突变体包括位置60,87,97,99,102,116,117,126,127,128,130,133,134,154,156,158,159,160,164,169的突变， 175，180，182，193，197，198，203，211和216.因此，新的蛋白酶非常适合用于各种类型的洗涤剂，无论是否与其他酶联合使用，例如淀粉酶，纤维素酶 和脂肪酶。 优选的实施方案是在102位具有突变的PB92和枯草杆菌蛋白酶309突变体，特别是具有至少一个其它突变的突变体。