公开(公告)号：WO1994018343A1

公开(公告)日：1994-08-18

申请号：PCT/EP1994000359

申请日：1994-02-09

Applicant: GIST-BROCADES N.V. ,  VAN RIJN, Ferdinand, Theodorus, Jozef ,  BEUKERS, Robert ,  KERKHOF, Johannes, H., P., M.

Inventor： GIST-BROCADES N.V.

IPC: C12Q01/18

CPC classification number: G01N33/493 ,  C12Q1/18 ,  G01N33/04 ,  G01N33/12 ,  Y10S435/832

Abstract: The detection of residues of antibacterials such as antibiotics and sulpha compounds in liquids such as milk, water, meat juice, serum or urine is disclosed. A test unit comprises an agar medium inoculated with a suitable test organism and two or more redox indicators.

Abstract translation: 公开了在液体如牛奶，水，肉汁，血清或尿液中检测抗菌素如抗生素和硫化合物的残留物。 测试单元包括用合适的测试生物接种的琼脂培养基和两种或更多种氧化还原指示剂。

公开(公告)号：WO1994002618A1

公开(公告)日：1994-02-03

申请号：PCT/EP1993001917

申请日：1993-07-19

Applicant: GIST-BROCADES N.V. ,  MULLENERS, Leonardus, Johannes, Sofie, Marie ,  MISSET, Onno ,  VAN DER LAAN, Jan, Metske ,  VAN GASTEL, Franciscus, Josephus, Cornelius ,  BROEKHUIZEN, Cornelis, Petrus ,  BAAS, Erik, Jan

Inventor： GIST-BROCADES N.V.

IPC: C12N15/57

CPC classification number: C11D3/386 ,  C12N9/54

Abstract: New PB92 or Subtilisin 309 mutant serine proteases are provided having specific mutations, resulting in a surprisingly better wash performance or in an improved storage stability with at similar or even better wash performance. These PB92 or Subtilisin 309 mutants include mutations at positions 60, 87, 97, 99, 102, 116, 117, 126, 127, 128, 130, 133, 134, 154, 156, 158, 159, 160, 164, 169, 175, 180, 182, 193, 197, 198, 203, 211, and 216. The new proteases, therefore, are very suitable for use in various types of detergents, whether or not in conjunction with other enzymes, for example amylases, cellulases and lipases. Preferred embodiments are the PB92 and Subtilisin 309 mutants having a mutation at position 102 and in particular those having at least one further mutation.

Abstract translation: 提供新的PB92或枯草杆菌蛋白酶309突变丝氨酸蛋白酶，其具有特异性突变，导致惊人的更好的洗涤性能或者在相似或甚至更好的洗涤性能下具有改善的储存稳定性。 这些PB92或枯草杆菌蛋白酶309突变体包括位置60,87,97,99,102,116,117,126,127,128,130,133,134,154,156,158,159,160,164,169的突变， 175，180，182，193，197，198，203，211和216.因此，新的蛋白酶非常适合用于各种类型的洗涤剂，无论是否与其他酶联合使用，例如淀粉酶，纤维素酶 和脂肪酶。 优选的实施方案是在102位具有突变的PB92和枯草杆菌蛋白酶309突变体，特别是具有至少一个其它突变的突变体。

公开(公告)号：WO1993020198A1

公开(公告)日：1993-10-14

申请号：PCT/EP1993000850

申请日：1993-04-05

Applicant: GIST-BROCADES N.V. ,  CERDA-OLMEDO, Enrique ,  MEHTA, Bina, Jamnadas ,  VAN DIJCK, Petrus, Wilhelmus, Maria ,  KOEKMAN, Bertus, Pieter

Inventor： GIST-BROCADES N.V.

IPC: C12N15/04

CPC classification number: C12N15/04 ,  C12N15/80 ,  C12N15/89 ,  C12P23/00

Abstract: The present invention discloses intersexual heterozygous Phycomyces strains. These strains show an improved beta -carotene productivity and an increased stability when compared with both the wild type and heterokaryotic Phycomyces strains. The present invention also disclosed a method for obtaining Phycomyces transformants without using selection markers. Furthermore, a process is provided for producing beta -carotene comprising the use of intersexual heterozygous Phycomyces strains in a submerged culture.

Abstract translation: 本发明公开了异种间杂种Phycomyces菌株。 与野生型和异源真菌种菌株相比，这些菌株显示出改善的β-胡萝卜素生产力和增加的稳定性。 本发明还公开了一种在不使用选择标记的情况下获得植物分枝杆菌转化体的方法。 此外，提供了一种生产β-胡萝卜素的方法，其包括在浸没培养物中使用间性杂合的Phycomyces菌株。

公开(公告)号：WO1993018179A1

公开(公告)日：1993-09-16

申请号：PCT/EP1993000514

申请日：1993-03-05

Applicant: GIST-BROCADES N.V. ,  VAN SOLINGEN, Pieter ,  STARK, Jacobus ,  LANGEVELD, Pieter, Cornelis

Inventor： GIST-BROCADES N.V.

IPC: C12P21/00

CPC classification number: A23C19/11 ,  A23L3/3571 ,  C07K14/195

Abstract: The present invention discloses a bacteriocin obtainable from Pediococcus cerevisiae P18. Said bacteriocin is composed of proteinaceous material and has a broad range of activity against Listeria monocytogenes. The bacteriocin is inactive against the tested starter and surface culture strains. The invention further provides a method for obtaining the bacteriocin. Finally, the use of the bacteriocin is illustrated.

Abstract translation: 本发明公开了一种可从小球乳杆菌P18获得的细菌素。 所述细菌素由蛋白质材料组成，对单核细胞增生利斯特氏菌具有广泛的活性。 细菌素对测试的起始物和表面培养菌株无活性。 本发明还提供了获得细菌素的方法。 最后，说明了细菌素的使用。

公开(公告)号：WO1993014645A1

公开(公告)日：1993-08-05

申请号：PCT/NL1993000025

申请日：1993-01-25

Applicant: GIST-BROCADES N.V ,  HAMSTRA, Reinder, Sietze ,  TROMP, Augustinus, Franciscus, Cornelis, Petrus, ...

Inventor： GIST-BROCADES N.V

IPC: A23K01/16

CPC classification number: A23K50/80 ,  A23K20/179 ,  A23K20/189 ,  A23K40/30

Abstract: The present invention discloses a method for obtaining feed pellets. The method comprises the addition of active ingredients to feed pellets after the pellets have been extruded. The method further comprises the addition of a solution or suspension of the desired feed or ingredient in a water or oil phase to the pellets under reduced pressure and subsequently increasing the pressure. The method results in pellets with a high degree of loading and in which the active ingredients are homogeneously distributed.

公开(公告)号：WO1992007079A1

公开(公告)日：1992-04-30

申请号：PCT/NL1991000203

申请日：1991-10-15

Applicant: GIST-BROCADES N.V. ,  VAN HARTINGSVELDT, Willem ,  VAN GORCOM, Robert, Franciscus, Maria ,  GOUKA, Robertus, Johannes ,  BOVENBERG, Roelof, Ary, Lans

Inventor： GIST-BROCADES N.V.

IPC: C12N15/80

CPC classification number: C12N9/93 ,  C12N15/80

Abstract: A transformant selection system has been developed, particularly for a β-lactam producing strain, more particularly for P. chrysogenum, by the complementation of a mutation of said β-lactam producing strain by a homologous selection marker without interfering with β-lactam biosynthesis. Particularly, in applying said transformant selection system a positive selection agent, for instance fluoroacetate is used for the isolation of fac mutants of said strain, particularly of said β-lactam producing strain. Furthermore, a gene entitled facA has been isolated from P. chrysogenum.

公开(公告)号：WO1991013154A1

公开(公告)日：1991-09-05

申请号：PCT/NL1991000033

申请日：1991-02-28

Applicant: GIST-BROCADES N.V.

Inventor： GIST-BROCADES N.V. ,  VAN DIJL, Jan, Maarten ,  SMITH, Hilde, E. ,  BRON, Sierd ,  QUAX, Wilhelmus, Johannes

IPC: C12N15/57

CPC classification number: C12N9/2417 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/61 ,  C12N9/52 ,  C12N9/86 ,  C12N15/625 ,  C12N15/67 ,  C12N15/70 ,  C12N15/75

Abstract: The subject invention describes the cloning and overexpression of leader peptidase genes. A method for isolating a leader peptidase gene is disclosed. Overexpression of the signal peptidase in a suitable host species leads to an enhanced rate of protein processing.

公开(公告)号：WO1991000353A2

公开(公告)日：1991-01-10

申请号：PCT/EP1990001042

申请日：1990-06-27

Applicant: GIST-BROCADES N.V. ,  PLANT GENETIC SYSTEMS N.V. ,  QUAX, Wilhelmus, Johannes ,  LAROCHE, Yves ,  VOLLEBREGT, Adrianus, Wilhelmus, Hermanus ,  STANSSENS, Patrick ,  LAUWEREYS, Marc

Inventor： GIST-BROCADES N.V. ,  PLANT GENETIC SYSTEMS N.V.

IPC: C12N15/56

CPC classification number: C12N15/75 ,  C12N9/2414 ,  C12N9/2417 ,  C12N15/70

Abstract: Thermostable and acid stable α-amylases are provided as expression products of genetically engineered α-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant α-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.

Abstract translation: 本发明涉及作为从微生物分离的基因工程α-淀粉酶基因表达产物的热稳定性和酸稳定性α-淀粉酶，所述微生物优选属于杆菌属（Bacilli）类。 化学和酶促诱变的方法例如是酸性亚硫酸盐过程以及酶促的erronic掺入间隔的异源双链体DNA。 突变α-淀粉酶在淀粉加工和纺织品退浆的工业应用中具有优异的性能，例如在宽pH范围内增强的热稳定性。

公开(公告)号：WO1985000382A1

公开(公告)日：1985-01-31

申请号：PCT/NL1984000021

申请日：1984-07-06

Applicant: GIST-BROCADES N.V.

Inventor： GIST-BROCADES N.V. ,  SANDERS, Johan, Pieter, Marinus ,  VAN DEN BERG, Johannes, Abel ,  ANDREOLI, Peter, Michael ,  VOS, Yvonne, Johanna ,  VAN EE, Jan, Hendrik ,  MULLENERS, Leo, J., S., M.

IPC: C12N15/00

CPC classification number: C12N9/2417 ,  C12N9/54 ,  C12N9/6481 ,  C12N15/63 ,  C12N15/75

Abstract: Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing one or more copies of the gene expressing the polypeptide of interest are introduced into a compatible host, the transformed hosts treated to form protoplasts and then fused with protoplast acceptors prepared from industrial strains. Efficient transfer of the vehicle containing the genes of interest is achieved, with the resulting modified industrial strains being effective producers of the desired polypeptide product.

公开(公告)号：WO1983002499A1

公开(公告)日：1983-07-21

申请号：PCT/NL1983000001

申请日：1983-01-14

Applicant: VISSER-LEEUW, Geeskelina, Sjuwkina (The Legal ... ,  GIST-BROCADES N.V. ,  STOLK, Izak, Johannes ,  BROUWER, Willem

Inventor： VISSER-LEEUW, Geeskelina, Sjuwkina (The Legal ... ,  GIST-BROCADES N.V. ,  VISSER, Hendrikus, Wilhelmus (deceased)

IPC: G01F11/28

CPC classification number: G01F3/38 ,  G01F11/28

Abstract: Un procédé de mesure de quantités de gaz et de distribution de quantités prédéterminées de liquides utilise un agencement de conteneurs connectés entre eux (2, 3, 5) et contenant des liquides et des moyens de commande (6, 7), le procédé consistant à introduire un gaz dans l'un (2) des conteneurs (2, 3, 5), ce qui fait monter le liquide dans un autre conteneur (la pipette de mesure (5)) jusqu'à un niveau prédéterminé (6) où une quantité de liquides s'écoule du système jusqu'à ce qu'un niveau prédéterminé plus bas (7) soit atteint, après quoi le liquide monte de nouveau, etc., et si on le désire le nombre de fois que la pipette de mesure (5) est remplie et vidée est compté par un compteur (21). Un appareil de mise en oeuvre de ce procédé est également décrit.