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    AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    3.
    发明申请
    AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
    AMIDASES，核酸编码它们及其制备和使用它们的方法

    公开(公告)号：WO2004069848A2

    公开(公告)日：2004-08-19

    申请号：PCT/US2003/028786

    申请日：2003-09-11

    Applicant: DIVERSA CORPORATION ,  BARTON, Nelson, Robert ,  WEINER, David, Paul ,  GREENBERG, William ,  LUU, Samantha ,  CHAN, Kristine ,  WATERS, Elizabeth

    Inventor： BARTON, Nelson, Robert ,  WEINER, David, Paul ,  GREENBERG, William ,  LUU, Samantha ,  CHAN, Kristine ,  WATERS, Elizabeth

    IPC: C07H

    CPC classification number: C12N9/80 ,  C12P35/02 ,  C12Q1/34

    Abstract: This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7­aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(E-D-2-aminoadipoylamido)-4-methylcoumarin.

    Abstract translation: 本发明提供酰胺酶，编码酰胺酶的多核苷酸，制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了具有仲酰胺酶活性的酶，例如在酰胺水解中具有活性，包括具有肽酶，蛋白酶和/或乙内酰脲酶活性的酶。 在替代方面，本发明的酶可用于增加食物中的风味（例如酶熟干酪），促进细菌和真菌杀死，改善和去保护精细化学中间体，合成肽键，进行手性拆分 ，水解头孢菌素C.本发明的酶可用于产生7氨基头孢烷酸（7-ACA）和半合成头孢菌素抗生素，包括沙司他啶，头孢菌啶和头孢呋辛。 本发明的酶可以用作抗微生物剂，例如细胞壁水解剂。 本发明还提供包含7-（E-D-2-氨基己二酰氨基）-4-甲基香豆素的荧光酰胺酶底物。

    AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    5.
    发明申请
    AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
    AMIDASES，核酸编码它们及其制备和使用它们的方法

    公开(公告)号：WO2004069848A3

    公开(公告)日：2007-12-21

    申请号：PCT/US0328786

    申请日：2003-09-11

    Applicant: DIVERSA CORP ,  BARTON NELSON ROBERT ,  WEINER DAVID PAUL ,  GREENBERG WILLIAM ,  LUU SAMANTHA ,  CHANG KRISTINE ,  WATERS ELIZABETH

    Inventor： BARTON NELSON ROBERT ,  WEINER DAVID PAUL ,  GREENBERG WILLIAM ,  LUU SAMANTHA ,  CHANG KRISTINE ,  WATERS ELIZABETH

    IPC: C12N15/00 ,  C07H21/04 ,  C07K14/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/14 ,  C12N9/24 ,  C12N9/48 ,  C12N9/78 ,  C12N9/80 ,  C12P21/06 ,  C12P35/02 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/68

    CPC classification number: C12N9/80 ,  C12P35/02 ,  C12Q1/34

    Abstract: This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7­aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(E-D-2-aminoadipoylamido)-4-methylcoumarin.

    Abstract translation: 本发明提供酰胺酶，编码酰胺酶的多核苷酸，制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了具有仲酰胺酶活性的酶，例如在酰胺水解中具有活性，包括具有肽酶，蛋白酶和/或乙内酰脲酶活性的酶。 在替代方面，本发明的酶可用于增加食物中的风味（例如酶熟干酪），促进细菌和真菌杀死，改善和去保护精细化学中间体，合成肽键，进行手性拆分 ，水解头孢菌素C.本发明的酶可用于产生7氨基头孢菌素酸（7-ACA）和半合成头孢菌素抗生素，包括沙司他啶，头孢菌啶和头孢呋辛。 本发明的酶可以用作抗微生物剂，例如细胞壁水解剂。 本发明还提供包含7-（E-D-2-氨基己二酰氨基）-4-甲基香豆素的荧光酰胺酶底物。

    AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    8.
    发明申请
    AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
    AMIDASES，核酸编码它们及其制备和使用它们的方法

    公开(公告)号：WO2003064613A2

    公开(公告)日：2003-08-07

    申请号：PCT/US2003/002694

    申请日：2003-01-28

    Applicant: DIVERSA CORPORATION ,  BARTON, Nelson, R. ,  WEINER, David, Paul ,  GREENBERG, William ,  LUU, Samantha ,  CHANG, Kristine ,  WATERS, Elizabeth

    Inventor： BARTON, Nelson, R. ,  WEINER, David, Paul ,  GREENBERG, William ,  LUU, Samantha ,  CHANG, Kristine ,  WATERS, Elizabeth

    IPC: C12N

    CPC classification number: C07D501/00 ,  A01K2217/05 ,  A61K38/00 ,  C12N9/78 ,  C12P35/00 ,  C12P35/02 ,  C12P41/007 ,  C12Q1/34

    Abstract: This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7-aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(ε-D-2-aminoadipoyladipoylamido)-4-methylcoumarin.

    Abstract translation: 本发明提供酰胺酶，编码酰胺酶的多核苷酸，制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了具有仲酰胺酶活性的酶，例如在酰胺水解中具有活性，包括具有肽酶，蛋白酶和/或乙内酰脲酶活性的酶。 在替代方面，本发明的酶可用于增加食物中的风味（例如酶熟干酪），促进细菌和真菌杀死，改善和去保护精细化学中间体，合成肽键，进行手性拆分 ，水解头孢菌素C.本发明的酶可用于产生7-氨基头孢烷酸（7-ACA）和半合成头孢菌素抗生素，包括沙司他啶，头孢菌啶和头孢呋辛。 本发明的酶可以用作抗微生物剂，例如细胞壁水解剂。 本发明还提供了包含7-（ε-D-2-氨基己二酰己二酰氨基）-4-甲基香豆素的荧光酰胺酶底物。

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