公开(公告)号：WO2017051930A1

公开(公告)日：2017-03-30

申请号：PCT/JP2016/079196

申请日：2016-09-26

Applicant: AJINOMOTO CO., INC.

Inventor： HOSHINO, Yasushi ,  TAJIMA, Yoshinori ,  RACHI, Hiroaki ,  OGATA, Fumi ,  KATASHKINA, Joanna Yosifovna

IPC: C12P5/02 ,  C12N15/09

CPC classification number: C12Y205/01 ,  C12P5/007 ,  C12P17/12 ,  C12Y101/9901

Abstract: The object of the present invention is to provide a method of efficiently producing an isoprenoid compound. More specifically, the present invention provides a method of producing an isoprenoid compound including culturing an isoprenoid compound-producing microorganism that has a dimethylallyl diphosphate or isopentenyl diphosphate supplying pathway and a blocked 2-ketogluconate formation pathway in a culture medium to form the isoprenoid compound, and the isoprenoid compound-producing microorganism that has a dimethylallyl diphosphate or isopentenyl diphosphate supplying pathway and a blocked 2-ketogluconate formation pathway.

Abstract translation: 本发明的目的是提供一种有效地制备类异戊二烯化合物的方法。 更具体地，本发明提供一种制备类异戊二烯化合物的方法，包括培养具有二甲基烯丙基二磷酸酯或异戊烯基二磷酸酯供应途径的异戊二烯化合物生产微生物和封闭的2-酮葡糖酸盐形成途径以形成类异戊二烯化合物， 和具有二甲基烯丙基二磷酸酯或异戊烯基二磷酸酯供应途径的异戊二烯化合物生产微生物和封闭的2-酮葡萄糖酸生成途径。

公开(公告)号：WO2015147341A3

公开(公告)日：2015-10-01

申请号：PCT/JP2015/060529

申请日：2015-03-27

Applicant: AJINOMOTO CO., INC.

Inventor： MIHARA, Yoko ,  RACHI, Hiroaki ,  NISHIO, Yosuke ,  KATASHKINA, Yosifovna Joanna ,  KAZIEVA, Dmitrievna Ekaterina ,  ANDREEVA, Georgievna Irina

IPC: C12N1/21 ,  C12N9/12 ,  C12P5/00 ,  C12P9/00

Abstract: A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from Methanocella, Corynebacterium, Methanosaeta , and Nitrosopumilus , and (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.