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公开(公告)号：WO0246238A3
公开(公告)日：2003-07-10
申请号：PCT/US0147656
申请日：2001-12-05
Applicant: ALEXION PHARMA INC
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA , RENSHAW MARK
IPC: A61K39/00 , C07K14/505 , C07K14/52 , C07K16/00 , C07K16/12 , C07K16/18 , C07K16/46 , C12N1/21 , C12N15/13 , C07K19/00 , A61K39/395 , C12N15/62 , C12N15/70 , G01N33/50 , G01N33/573
CPC classification number: C07K14/505 , A61K39/00 , A61K39/40 , A61K2039/505 , C07K14/524 , C07K16/00 , C07K16/005 , C07K16/1282 , C07K16/18 , C07K16/46 , C07K2317/21 , C07K2317/55 , C07K2317/565 , C07K2317/74 , C07K2318/10 , C07K2319/00 , C07K2319/036 , C07K2319/30 , C07K2319/75 , C12N5/0641 , C12N2501/14 , C12N2506/11
Abstract: Antibodies or fragments thereof having CDR regions replaced or fused with biologically active peptides are described. Flanking sequences may optionally be attached at one or both the carboxy-terminal and amino-terminal ends of the peptide in covalent association with adjacent framework regions. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.
Abstract translation: 描述了具有替换或与生物活性肽融合的CDR区的抗体或其片段。 侧翼序列可以任选地在肽的羧基末端和氨基末端的一个或两个连接，与相邻构架区共价结合。 含有此类抗体或其片段的组合物可用于治疗和诊断方式。
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公开(公告)号：WO0246435A3
公开(公告)日：2003-06-12
申请号：PCT/US0147452
申请日：2001-12-05
Applicant: ALEXION PHARMA INC
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA , LIN YING-CHI , RENSHAW MARK , WILD MARTHA , MCWHIRTER JOHN
CPC classification number: C12N15/1096 , C07K16/00 , C07K2317/50 , C12N15/64 , C12N15/66
Abstract: Nucleic acid sequences encoding at least a portion of a polypeptide are directly incorporated into a plasmid by DNA polymerization or reverse transcription of a nucleic acid template. In particularly preferred embodiments, nucleic acid sequences encoding at least a portion of an antibody are directly incorporated into a plasmid by reverse transcription of messenger RNA (mRNA).
Abstract translation: 编码多肽的至少一部分的核酸序列通过核酸模板的DNA聚合或逆转录直接掺入质粒。 在特别优选的实施方案中，编码抗体的至少一部分的核酸序列通过信使RNA（mRNA）的逆转录直接并入质粒。
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Patent Agency Ranking
公开(公告)号：WO0246436A2
公开(公告)日：2002-06-13
申请号：PCT/US0146516
申请日：2001-12-07
Applicant: ALEXION PHARMA INC , BOWDISH KATHERINE S , BARBAS FREDERICKSON SHANA , WILD MARTHA , MCWHIRTER JOHN
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA , WILD MARTHA , MCWHIRTER JOHN
IPC: C12N15/72
CPC classification number: C12N15/72
Abstract: This disclosure describes novel vectors capable or replication and expression of foreign genetic information in bacteria, such as, for example, cyanobacterium and E. coli. This disclosure also deals with the use of the vectors to introduce foreign genes into bacteria.
Abstract translation: 本公开描述了能够或复制和在细菌中例如蓝细菌和大肠杆菌表达外源遗传信息的新载体。 本公开还涉及载体将外源基因引入细菌中的用途。
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公开(公告)号：WO03093471A8
公开(公告)日：2004-07-22
申请号：PCT/US0214971
申请日：2002-04-29
Applicant: ALEXION PHARMA INC
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA
CPC classification number: C40B40/02 , C07H21/04 , C12N15/1037
Abstract: A phage genome is engineered to include a novel restriction site at one of two different positions. In a first embodiment, a restriction site is inserted into the phage genome between the end of gene IV and the MOS hairpin which serves as a phage packaging signal for newly synthesized single strands of phage DNA. In a second embodiment, a restriction site is inserted into the phage genome after the MOS hairpin and prior to the minus strand origin. Once the phage genome is modified to contain the new restriction site, the vector can be engineered to be a "88" vector by inserting at the new restriction site a nucleotide sequence encoding at least a functional domain of pVIII and at least a first cloning site for receiving a gene encoding a polypeptide to be displayed and, optionally a second cloning site for receiving to be displayed. In particularly useful embodiments, the novel vectors are engineered to produce phage particles that display antibodies.
Abstract translation: 噬菌体基因组被设计为在两个不同位置之一包括新的限制性位点。 在第一实施方案中，将限制性位点插入到基因IV的末端和用作新合成的噬菌体DNA单链的噬菌体包装信号的MOS发夹之间的噬菌体基因组中。 在第二个实施方案中，将限制性位点插入到MOS发夹后并且在负链起始之前的噬菌体基因组中。 一旦噬菌体基因组被修饰以含有新的限制性位点，可以将该载体设计成“88”载体，通过在新的限制性位点插入编码至少pVIII的功能结构域的核苷酸序列和至少第一个克隆位点 用于接收编码要显示的多肽的基因，以及任选的用于接收待显示的第二克隆位点。 在特别有用的实施方案中，新载体被工程化以产生显示抗体的噬菌体颗粒。
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公开(公告)号：WO0246434A3
公开(公告)日：2004-03-25
申请号：PCT/US0146514
申请日：2001-12-07
Applicant: ALEXION PHARMA INC , BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA
CPC classification number: C12N15/1037 , C07K16/00 , C07K2317/622 , C12N15/70 , C40B40/02
Abstract: Phagemid vectors incorporating dimerization domains are provided which allow efficient production of biologically active polypeptides that may require dimerization for their biological activity.
Abstract translation: 提供了结合二聚化结构域的噬菌体载体，其允许有效生产生物活性多肽，其可能需要二聚化用于其生物学活性。
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6.发明申请
公开(公告)号：WO0248401A3
公开(公告)日：2003-09-12
申请号：PCT/US0147727
申请日：2001-12-10
Applicant: ALEXION PHARMA INC
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA , LIN YING-SHI , MCWHIRTER JOHN , MARUYAMA TOSHIAKI
CPC classification number: C12Q1/6844 , C12Q2525/301
Abstract: Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library a polypeptides or proteins, such as, for example, an antibody library.
Abstract translation: 通过嵌套的寡核苷酸延伸反应提供被设计成含有预定序列和发夹结构的模板。 工程模板允许单引物扩增（SPA）扩增工程模板内的靶序列。 在特别有用的实施方案中，将来自工程化模板的靶序列克隆到表达载体中以向文库提供多肽或蛋白质，例如抗体文库。
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公开(公告)号：WO0246436A9
公开(公告)日：2003-04-17
申请号：PCT/US0146516
申请日：2001-12-07
Applicant: ALEXION PHARMA INC , BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA , WILD MARTHA , MCWHIRTER JOHN
Inventor： BOWDISH KATHERINE S , BARBAS-FREDERICKSON SHANA , WILD MARTHA , MCWHIRTER JOHN
CPC classification number: C12N15/72
Abstract: This disclosure describes novel vectors capable or replication and expression of foreign genetic information in bacteria, such as, for example, cyanobacterium and E. coli. This disclosure also deals with the use of the vectors to introduce foreign genes into bacteria.
Abstract translation: 本公开描述了能够或复制和在细菌中例如蓝细菌和大肠杆菌表达外源遗传信息的新载体。 本公开还涉及载体将外源基因引入细菌中的用途。
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