公开(公告)号：WO1988002004A1

公开(公告)日：1988-03-24

申请号：PCT/US1986001970

申请日：1986-09-20

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  FUNG, Steven ,  WOO, Sam, Lee ,  SMITH, Lloyd, M.

IPC: C07F09/22

CPC classification number: C07F9/2416 ,  A61K47/545 ,  A61K47/548 ,  C07F9/2408 ,  C07F9/65842 ,  C07H21/00 ,  C08B37/0021 ,  C08B37/0039

Abstract: The compounds include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention further include conjugates of the above linking agents with oligonucleotides and polymer supports. The compounds of the present invention are useful for linking organic moities, such as fluorescent or chromogenic dyes, to polymer supports and oligonucleotides, particularly single- and double-stranded DNA and RNA fragments.

公开(公告)号：WO1991007507A1

公开(公告)日：1991-05-30

申请号：PCT/US1990006608

申请日：1990-11-13

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  MENCHEN, Steven, M. ,  LEE, Linda, G. ,  CONNELL, Charles, R. ,  HERSHEY, N., Davis ,  CHAKERIAN, Vergine ,  WOO, Sam, Lee ,  FUNG, Steven

IPC: C12Q01/68

CPC classification number: C12Q1/6869 ,  C07D311/82 ,  C07H21/00 ,  C09B11/08 ,  C12Q1/6816 ,  Y10S435/968 ,  Y10S436/80 ,  C12Q2563/107 ,  C12Q2535/101

Abstract: Long wavelength, narrow emission bandwidth fluorescein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1',2',7',8'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are described.

公开(公告)号：WO1993020239A1

公开(公告)日：1993-10-14

申请号：PCT/US1993003229

申请日：1993-04-02

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  GROSSMAN, Paul, David ,  FUNG, Steven ,  MENCHEN, Steven, Michael ,  WOO, Sam, Lee ,  WINN-DEEN, Emily, Susan

IPC: C12Q01/68

CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2525/203 ,  C12Q2525/197 ,  C12Q2563/167 ,  C12Q2535/101 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2531/137 ,  C12Q2563/119 ,  C12Q2549/119 ,  C12Q2525/161 ,  C12Q2521/501 ,  C12Q2563/131 ,  C12Q2525/101 ,  C12Q2531/113

Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In practicing the method, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive elctrophoretic mobility in a sieving matrix, to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

Abstract translation: 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在实施该方法中，将多个不同序列探针对加入到靶多核苷酸中，其中每个探针对包含两个多核苷酸探针元件，其与靶多核苷酸中选定的一个靶序列的相邻部分在序列上互补。 在每个探针对中，探针元件中的一个包含非多核苷酸聚合物链，其在筛选基质中赋予相关探针对中的独特的电泳迁移率，当该对中的元件被连接时。 该对中的另一个元素包含可检测的报告物标记。 在探针对已被允许与靶多核苷酸杂交后，杂交的多核苷酸在有效连接靶结合探针元件的末端亚基的条件下处理，当它们的末端亚基与相邻的靶基碱基配对时。 然后将连接的探针对从目标多核苷酸中释放，并在筛分基质中电泳分离，或进行色谱分离。

公开(公告)号：WO1993020236A1

公开(公告)日：1993-10-14

申请号：PCT/US1993003048

申请日：1993-03-31

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  GROSSMAN, Paul, David ,  FUNG, Steven ,  MENCHEN, Steven, Michael ,  WOO, Sam, Lee ,  WINN-DEEN, Emily, Susan

IPC: C12Q01/68

CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2525/203 ,  C12Q2525/197 ,  C12Q2563/167 ,  C12Q2535/101 ,  C12Q2565/629 ,  C12Q2563/155 ,  C12Q2563/107 ,  C12Q2531/137 ,  C12Q2563/119 ,  C12Q2549/119 ,  C12Q2525/161 ,  C12Q2521/501 ,  C12Q2563/131 ,  C12Q2525/101 ,  C12Q2531/113

Abstract: Method and composition for distinguishing different-sequence polynucleotides electrophoretically in a non-sieving medium. In practicing the method, there are formed one or more different-sequence polynucleotide(s) which contain (i) a detectable reporter label and (ii) an attached polymer chain which imparts to each different-sequence polynucleotide, a distinctive ratio of charge/translational frictional drag. The different-sequence polynucleotides which are formed are fractionated by capillary electrophoresis in a non-sieving matrix and detected according to their observed electrophoretic migration rates. The method can be used for DNA sequencing and for detecting one or more selected sequences in a sample.

Abstract translation: 在非筛选培养基中电泳鉴别不同序列多核苷酸的方法和组合物。 在实施该方法中，形成一种或多种不同序列的多核苷酸，其包含（i）可检测的报告基因标记和（ii）赋予每个不同序列多核苷酸的独立的充电/ 平移摩擦阻力。 形成的不同序列多核苷酸通过毛细管电泳在非筛选基质中分级，并根据其观察到的电泳迁移率进行检测。 该方法可用于DNA测序和检测样品中一个或多个选定的序列。