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    SINGLE-MOLECULE PHENOTYPING AND SEQUENCING OF NUCLEIC ACID MOLECULES
    2.
    发明申请
    SINGLE-MOLECULE PHENOTYPING AND SEQUENCING OF NUCLEIC ACID MOLECULES 审中-公开

    公开(公告)号：WO2019053430A1

    公开(公告)日：2019-03-21

    申请号：PCT/GB2018/052598

    申请日：2018-09-13

    申请人： OXFORD UNIVERSITY INNOVATION LTD.

    发明人： KAPANIDIS, Achillefs ,  ANDREWS, Rebecca

    IPC分类号： C12Q1/6874

    CPC分类号： C12Q1/6874 ,  C12Q2525/101 ,  C12Q2549/113 ,  C12Q2563/107 ,  C12Q2565/501

    摘要： This invention relates to a method of sequencing a nucleic acid molecule, in particular the method comprising: (a) providing the nucleic acid immobilised on a surface; (b) forming a single-stranded gap section by partially duplexing the nucleic acid such that the single-stranded gap section is flanked by duplex sections, wherein the sequence to be sequenced is a sequence of the single-stranded gap section; (c) providing a set of at least four fluorescently labelled oligonucleotide probes, (d) detecting binding, or absence thereof, of the fluorescently labelled oligonucleotide probes with the gap section of the immobilised nucleic acid, wherein the identity of the interrogated nucleotide of the gap section of the immobilised nucleic acid is identified as the complementary base of the nucleotide X of the fluorescently labelled oligonucleotide probe that has the highest incidence of binding; (e) repeating steps (c) and (d) for interrogating subsequent nucleotide positions of the gap section of the immobilised nucleic acid until sufficient nucleotides of the gap section have been identified to be able to determine a sequence. Also provided are methods of single-molecule phenotyping and sequencing; methods of single-molecule phenotyping and identification of a molecule tagged with nucleic acid; an immobilised nucleic acid molecule; and a composition comprising oligonucleotide probes.

    DUAL DETECTION SCHEME FOR DNA SEQUENCING
    7.
    发明申请
    DUAL DETECTION SCHEME FOR DNA SEQUENCING 审中-公开
    DNA测序的双重检测方案

    公开(公告)号：WO2018034982A1

    公开(公告)日：2018-02-22

    申请号：PCT/US2017/046500

    申请日：2017-08-11

    申请人： CBRITE INC.

    发明人： GUNNING, Kerry ,  ACKLEY, Donald ,  SHIEH, Chan-long

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： G01N21/6428 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2021/6439 ,  G01N2201/062 ,  C12Q2563/107 ,  C12Q2565/607

    摘要： Apparatus for fluorescent and ion sensing of DNA nucleotide incorporation events including DNA nucleotide incorporation structure designed to have sequencing primers bonded to a surface for the incorporation of DNA nucleotides thereon. At least some of the DNA nucleotides having a fluorescent label. A photodiode positioned adjacent the incorporation structure and an illumination device positioned adjacent the DNA nucleotide incorporation structure to illuminate DNA nucleotides incorporated onto the sequencing primers. The illumination device exciting the fluorescent labels when incorporation occurs and the photodiode positioned to sense the excited fluorescent labels. Ion sensing apparatus positioned adjacent the DNA nucleotide incorporation structure including a metal oxide thin film transistor with a gate electrically coupled to receive an electrical signal indicative of ion emissions produced by the DNA nucleotide incorporated onto DNA target fragments or sequencing primers.

    摘要翻译： 用于DNA核苷酸掺入事件的荧光和离子感应的装置，包括DNA核苷酸掺入结构，其被设计为具有结合到表面以将DNA核苷酸掺入其上的测序引物。 至少一些具有荧光标记的DNA核苷酸。 位于掺入结构附近的光电二极管和位于DNA核苷酸掺入结构附近的照明装置，以照射掺入到测序引物中的DNA核苷酸。 照明装置在结合时激发荧光标签并且光电二极管定位为感测激发的荧光标签。 离子感测装置位于DNA核苷酸掺入结构附近，包括金属氧化物薄膜晶体管，其栅极电耦合以接收指示由掺入DNA靶片段或测序引物的DNA核苷酸产生的离子发射的电信号。

    METHODS FOR DETECTING AND IDENTIFYING GENOMIC NUCLEIC ACIDS
    10.
    发明申请
    METHODS FOR DETECTING AND IDENTIFYING GENOMIC NUCLEIC ACIDS 审中-公开
    检测和鉴定基因组核酸的方法

    公开(公告)号：WO2017161251A1

    公开(公告)日：2017-09-21

    申请号：PCT/US2017/022942

    申请日：2017-03-17

    申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

    发明人： DAUGHARTHY, Evan R. ,  NGUYEN, Sonny C. ,  WU, Chao-ting ,  CHURCH, George M.

    IPC分类号： C12Q1/68 ,  C40B40/08 ,  C40B70/00

    CPC分类号： C12Q1/6841 ,  C12Q1/6816 ,  C40B40/08 ,  C40B70/00 ,  C12Q2535/00 ,  C12Q2537/143 ,  C12Q2563/107 ,  C12Q2565/514

    摘要： The present invention relates to a method of identifying a target genomic nucleic acid sequence including hybridizing a set of probes to the target genomic nucleic acid sequence, wherein the set of probes has a unique associated barcode sequence for identification of the target genomic nucleic acid sequence, wherein each probe of the set includes (1) a complementary sequence complementary to a first strand of the target genomic nucleic acid sequence and (2) the associated barcode sequence or a portion of the associated barcode sequence, sequencing the associated barcode sequence from probes hybridized to the target genomic nucleic acid sequence using a fluorescence-based sequencing method, and identifying the target genomic nucleic acid sequence by the sequenced barcode sequence.

    摘要翻译： 本发明涉及一种鉴定靶基因组核酸序列的方法，包括将一组探针与靶基因组核酸序列杂交，其中该组探针具有独特的相关联的用于鉴定的条形码序列 （1）与靶基因组核酸序列的第一链互补的互补序列和（2）相关的条形码序列或相关条形码序列的一部分，其中所述靶基因组核酸序列中的每个探针包含 使用基于荧光的测序方法对来自与目标基因组核酸序列杂交的探针的相关条形码序列进行测序，并通过测序条形码序列鉴定靶基因组核酸序列。