公开(公告)号：WO2002064610A1

公开(公告)日：2002-08-22

申请号：PCT/US2002/003469

申请日：2002-02-08

Applicant: ARCHER-DANIELS-MIDLAND COMPANY

Inventor： LI, Lhing-Yew ,  TREI, Kelli, Jean

IPC: C07H21/02

CPC classification number: C12N15/52 ,  C12P13/08

Abstract: The invention relates to production of lysine, and provides several isolated polynucleotide molecules useful for the production of L-lysine. One such polynucleotide encodes an aspartate kinase (ask), an aspartate-semialdehyde dehydrogenase (asd) and a dihydrodipicolinate reductase (dapB). Other polypeptides encode ask, asd, dapB and a diaminopimelate dehydrogenase (ddh); ask, asd, dapB, ddh and an ORF2 poypeptide; and ask, asd dapB, ddh, ORF2 and a diaminopimelate decarboxylase (lysA). The invention further provides methods of making and using the polynucleotides, and methods to increase the production of L-lysine.

Abstract translation: 本发明涉及赖氨酸的生产，并且提供了可用于生产L-赖氨酸的若干分离的多核苷酸分子。 一种这样的多核苷酸编码天冬氨酸激酶（ask），天冬氨酸半醛脱氢酶（asd）和二氢吡啶二羧酸还原酶（dapB）。 其他多肽编码ask，asd，dapB和二氨基庚二酸脱氢酶（ddh）; 问，asd，dapB，ddh和ORF2肽; 并询问asd dapB，ddh，ORF2和二氨基庚二酸脱羧酶（lysA）。 本发明还提供了制备和使用多核苷酸的方法以及增加L-赖氨酸生产的方法。